Mena Antibody (3E6) - Azide and BSA Free

Western Blot: Mena Antibody (3E6) [H00055740-M01] - Analysis of ENAH expression in transfected 293T cell line by ENAH monoclonal antibody (M01), clone 3E6.Lane 1: ENAH transfected lysate (Predicted MW: 66.5 KDa).Lane 2: Non-transfected lysate.

Sandwich ELISA: Mena Antibody (3E6) [H00055740-M01] - Detection limit for recombinant GST tagged ENAH is 3 ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA
• Clone: 3E6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Mena Antibody (3E6) - Azide and BSA Free Summary

• Immunogen: ENAH (NP_001008493.1, 291 a.a. ~ 390 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. LQAASQPAETPSQQGIVLGPLAPPPPPPLPPGPAQASVALPPPPGPPPPPPLPSTGPPPPPPPPPLPNQVPPPPPPPPAPPLPASGFFLASMSEDNRPLT
• Specificity: Reacts with enabled homolog (Drosophila).
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: ENAH
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Sandwich ELISA
  • Western Blot
• Application Notes: This antibody is reactive against recombinant protein in western blot and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Mena Antibody (3E6) - Azide and BSA Free

• ENA
• enabled homolog (Drosophila)
• ENAH
• FLJ10773
• Mena
• MENANDPP1
• NDPP1
• protein enabled homolog

Background

ENAH/MENA belongs to the Ena/vasodilator-stimulated phosphoprotein (VASP) family of proteins which play a role in regulating the actin cytoskeleton for the control of cell shape and movement. The Ena/VASP family of actin binding proteins includes ENAH/MENA which is the human homolog of drosophila Ena (enabled), VASP, and EVL (Ena/vasodilator-stimulated phosphoprotein-like protein). Ena/VASP proteins contain an EVH1 domain, a central proline-rich domain, and a terminal EVH2 domain. ENAH/MENA is not expressed in normal breast but has been detected and associated with breast lesions with a high risk of transformation and primary and metastatic breast cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ENAH
• Entrez:
  • 55740(Human)
• Uniprot:
  • NP_001008493(Human)