Melanoma HMB45 + MART1 + Tyrosinase Antibody (SC56-02)

Western Blot: Melanoma HMB45 + MART1 + Tyrosinase Antibody (SC56-02) [NBP2-67589] - Western blot analysis of Melanoma HMB45 + MART1 + Tyrosinase on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperat

Immunohistochemistry-Paraffin: Melanoma HMB45 + MART1 + Tyrosinase Antibody (SC56-02) [NBP2-67589] - Immunohistochemical analysis of paraffin-embedded human melanoma tissue using anti-Melanoma HMB45 + MART1 + Tyrosinase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-47, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Flow Cytometry: Melanoma HMB45 + MART1 + Tyrosinase Antibody (SC56-02) [NBP2-67589] - Analysis of B16-F1 cells with MelanA antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the seconda antibody

• Reactivity: Hu, Mu
• Applications: WB, Flow, IHC
• Clone: SC56-02
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

Melanoma HMB45 + MART1 + Tyrosinase Antibody (SC56-02) Summary

• Description: Novus Biologicals Rabbit Melanoma HMB45 + MART1 + Tyrosinase Antibody (SC56-02) (NBP2-67589) is a recombinant monoclonal antibody validated for use in IHC, WB and Flow. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human Melanoma HMB45 + MART1 + Tyrosinase aa 2-43 / 118. (SwissProt: Q16655 Human)
• Localization: Golgi apparatus, trans-Golgi network membrane, Endoplasmic reticulum membrane, Melanosome.
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: MLANA
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Melanoma HMB45 + MART1 + Tyrosinase Antibody (SC56-02)

• Antigen LB39-AA
• Antigen SK29-AA
• MART1MART-1
• melan-A
• melanoma antigen recognized by T-cells 1
• Protein Melan-A

Background

The combination of HMB45, MART-1 (DT101 + BC199) and Tyrosinase (T311) make this quadruple antibody cocktail a first-order pan melanoma screener, and may prove to be a valuable marker for melanoma metastasis in sentinel lymph nodes (see reference 3.).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MLANA