MEF2D Antibody

Western Blot: MEF2D Antibody [NBP1-80567] - Whole cell lysate from HeLa (15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), Jurkat (J; 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-MEF2D antibody used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. MEF2D was also immunoprecipitated by rabbit anti-MEF2D antibody NBP1-80566, which recognizes an upstream epitope.

Immunohistochemistry-Paraffin: MEF2D Antibody [NBP1-80567] - Section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti- MEF2D used at a dilution of 1:200 (1ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).

Immunohistochemistry-Paraffin: MEF2D Antibody [NBP1-80567] - FFPE serial sections of human breast carcinoma. Antibody: Affinity purified rabbit anti-MEF2D, left image and 1, right image) used at a dilution of 1:200 (1ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

MEF2D Antibody Summary

• Immunogen: The immunogen this antibody was made to, maps to a region between residue 475 and 521 of human Myocyte Enhancer Factor 2D using the numbering given in entry NP_005911.1 (GeneID 4209).
• Predicted Species: Rat (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MEF2D
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100 - 1:500
  • Immunohistochemistry-Paraffin 1:100- 1:500
  • Immunoprecipitation 2-10 ug/mg
  • Western Blot 1:2000-1:10000
• Application Notes: Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for MEF2D Antibody

• DKFZp686I1536
• MADS box transcription enhancer factor 2, polypeptide D (myocyte enhancerfactor 2D)
• MEF2D/DAZAP1 fusion
• myocyte enhancer factor 2D
• myocyte enhancer factor 2D/deleted in azoospermia associated protein 1 fusionprotein
• myocyte-specific enhancer factor 2D

Background

Transcriptional activator which binds specifically to the MEF2 element, 5'-YTA[AT](4)TAR-3', found in numerous muscle-specific, growth factor- and stress-induced genes. Mediates cellular functions not only in skeletal and cardiac muscle development, but also in neuronal differentiation and survival. Plays diverse roles in the control of cell growth, survival and apoptosis via p38 MAPK signaling in muscle-specific and/or growth factor-related transcription. Plays a critical role in the regulation of neuronal apoptosis

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MEF2D Antibody (NBP1-80567)(1)

Publication using NBP1-80567

• G�nczi M, Teixeira JMC, Barrera-Vilarmau S et al. Alternatively spliced exon regulates context-dependent MEF2D higher-order assembly during myogenesis Nature communications 2023-03-10 [PMID: 36898987] (Chromatin Immunoprecipitation (ChIP), Mouse)

Bioinformatics

• Gene Symbol: MEF2D
• Uniprot:
  • NP_005911.1(Human)