MED23 Antibody

Images

 
Western Blot: MED23 Antibody [NB200-339] - Nuclear extract (5 and 25 ug) from HeLa cells. Antibody used at 0.33 ug/ml.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IP (-)
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

MED23 Antibody Summary

Immunogen
between residues 1300 and the C-terminus of human MED23 protein
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MED23
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:5000
Application Notes
Immunoprecipitation Not recommended, antibodies NB200-337 and NB200-338 are recommened for IP.
Theoretical MW
156 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
HeLa Nuclear Cell Lysate
Publications
Read Publication using
NB200-339 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for MED23 Antibody

  • 130 kDa transcriptional co-activator
  • 133 kDa transcriptional co-activator
  • CRSP130
  • DRIP130DKFZp434H0117
  • mediator complex subunit 23CRSP133
  • Protein sur-2 homolog
  • subunit 3, 130kDa
  • Transcriptional coactivator CRSP130
  • Vitamin D3 receptor-interacting protein complex 130 kDa component

Background

The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. This protein also acts as a metastasis suppressor. Two alternatively spliced transcript variants encoding different isoforms have been described for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MED23 Antibody (NB200-339)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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WB
(1)
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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Research Areas for MED23 Antibody (NB200-339)

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Blogs on MED23

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Contact Information

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Calculators

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Bioinformatics

Gene Symbol MED23
Entrez
Uniprot