MED12 Antibody - BSA Free

Western Blot: MED12 Antibody - BSA Free [NBP3-45941] - Mouse liver tissue were subjected to SDS PAGE followed by western blot with (MED12 antibody) at dilution of 1:800

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

MED12 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit MED12 Antibody - BSA Free (NBP3-45941) is a polyclonal antibody validated for use in WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: mediator complex subunit 12. (Uniprot# Q93074)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MED12
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Western Blot 1:500-1:2000
• Theoretical MW: 230 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS, 50% Glycerol, pH (7.3)
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for MED12 Antibody - BSA Free

• ARC240
• CAG repeat protein 45
• CAGH45trinucleotide repeat containing 11 (THR-associated protein, 230kDa subunit)
• HOPAFGS1
• KIAA0192FG syndrome 1
• mediator complex subunit 12OPA-containing protein
• OKSsubunit 12 homolog
• thyroid hormone receptor-associated protein, 230 kDa subunit
• Trap230
• TRAP230mediator of RNA polymerase II transcription, subunit 12 homolog (S. cerevisiae)
• trinucleotide repeat containing 11 (THR-associated protein, 230 kDa subunit)
• Trinucleotide repeat-containing gene 11 protein

Background

The initiation of transcription is controlled in part by a large protein assembly known as the preinitiation complex. A component of this preinitiation complex is a 1.2 MDa protein aggregate called Mediator. This Mediator component binds with a CDK8 subcomplex which contains the protein encoded by this gene, mediator complex subunit 12 (MED12), along with MED13, CDK8 kinase, and cyclin C. The CDK8 subcomplex modulates Mediator-polymerase II interactions and thereby regulates transcription initiation and reinitation rates. The MED12 protein is essential for activating CDK8 kinase. Defects in this gene cause X-linked Opitz-Kaveggia syndrome, also known as FG syndrome, and Lujan-Fryns syndrome. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MED12