ME2 Antibody (S04-7I2)

Western Blot: ME2 Antibody (S04-7I2) [NBP3-15058] - Western blot detection of ME2 in K562, Hela cell lysates using ME2 Rabbit mAb (1:1000 diluted). Predicted band size: 65kDa. Observed band size: 65kDa.

Immunocytochemistry/Immunofluorescence: ME2 Antibody (S04-7I2) [NBP3-15058] - Immunofluorescence of ME2 (green) in Hela cells using NBP3-15058 at dilution 1/50, and DAPI (blue)

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clone: S04-7I2
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

ME2 Antibody (S04-7I2) Summary

• Description: Novus Biologicals Rabbit ME2 Antibody (S04-7I2) (NBP3-15058) is a recombinant monoclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthetic peptide of human ME2 (Uniprot#: P23368)
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: ME2
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1/20-1/50
  • Western Blot 1/1000-1/5000
• Theoretical MW: 65 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA
• Preservative: 0.01% Sodium Azide
• Purity: Affinity purified

Alternate Names for ME2 Antibody (S04-7I2)

• EC 1.1.1
• EC 1.1.1.38
• malate dehydrogenase
• Malic enzyme 2
• malic enzyme 2, NAD(+)-dependent, mitochondrial
• NAD-dependent malic enzyme, mitochondrial
• NAD-ME
• ODS1
• pyruvic-malic carboxylase

Background

The ME2 gene encodes a mitochondrial NAD-dependent malic enzyme, a homotetrameric protein, that catalyzes the oxidative decarboxylation of malate to pyruvate. It had previously been weakly linked to a syndrome known as Friedreich ataxia that has since been s

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ME2