ME1 Antibody - BSA Free

Analysis in human cell line CACO-2.

Staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts.

Staining of human kidney shows no positivity in cells in tubules as expected.

Staining of human cerebellum shows weak cytoplasmic positivity in Purkinje cells.

Staining of human stomach shows moderate cytoplasmic positivity in glandular cells.

Immunohistochemistry analysis in human testis and kidney tissues using HPA006493 antibody. Corresponding ME1 RNA-seq data are presented for the same tissues.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

ME1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit ME1 Antibody - BSA Free (NBP1-86129) is a polyclonal antibody validated for use in IHC and WB. Anti-ME1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: RITKNKLSDQTILFQGAGEAALGIAHLIVMALEKEGLPKEKAIKKIWLVDSKGLIVKGRASLTQEKEKFAHEHEEMKNLEAIVQEIKPTALIGVAAIGGAFSEQILKDMAAFNERPIIFALSNPTSKAEC
• Predicted Species: Mouse (92%), Rat (93%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ME1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.
• Control Peptide:

  ME1 Protein (NBP1-86129PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for ME1 Antibody - BSA Free

• EC 1.1.1
• EC 1.1.1.40
• HUMNDME
• malate dehydrogenase
• Malic enzyme 1
• malic enzyme 1, NADP(+)-dependent, cytosolic
• malic enzyme 1, soluble
• Malic enzyme, cytoplasmic
• MES
• NADP-dependent malic enzyme
• NADP-ME
• pyruvic-malic carboxylase

Background

ME1 encodes a cytosolic, NADP-dependent enzyme that generates NADPH for fatty acid biosynthesis. The activity of this enzyme, the reversible oxidative decarboxylation of malate, links the glycolytic and citric acid cycles. The regulation of expression for this gene is complex. Increased expression can result from elevated levels of thyroid hormones or by higher proportions of carbohydrates in the diet.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ME1 Antibody (NBP1-86129)(1)

Publication using NBP1-86129

• Kato BS, Nicholson G, Neiman M et al. Variance decomposition of protein profiles from antibody arrays using a longitudinal twin model. Proteome Sci 2011-11-17 [PMID: 22093360]

Bioinformatics

• Gene Symbol: ME1