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MDR1/ABCB1 Antibody

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Western Blot: MDR1/ABCB1 Antibody [NB100-80870] - Analyis of anti-ABCB1 antibody shows detection of ABCB1 in crude membrane extracts from HF insect cells over-expressing human ABCB1. The extract was loaded onto a gel in the amounts indicated followed by electrophoresis and transfer to nitrocellulose. The membrane was probed with the primary antibody diluted to 1:600, followed by Peroxidase Conjugated Affinity Purified Anti-RABBIT IgG at 1:10000. Personal Communication, Anna Calcagno, CCR-NCI, Bethesda, MD.
Western Blot: MDR1/ABCB1 Antibody [NB100-80870] - Analyis of anti-ABCB1 antibody shows detection of ABCB1 in crude membrane extracts from HF insect cells over-expressing human ABCB1. The extract was loaded onto a gel in ...read more
Immunohistochemistry-Paraffin: MDR1/ABCB1 Antibody [NB100-80870] - Staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, MkSpecies Glossary
Applications WB, ELISA, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

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MDR1/ABCB1 Antibody Summary

Immunogen
Synthetic peptide from human ABCB1 / P Glycoprotein.
Specificity
Amino acids 262-277 of human ABCB1 protein.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ABCB1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:200-1:2000
  • ELISA 1:10000-1:50000
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
Application Notes
IHC: NB100-80870 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity.
Control
MDR1/ABCB1 Lysate (NBL1-07172)
Reviewed Applications
Read 1 Review rated 5
using
NB100-80870 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20mM Potassium Phosphate (pH 7.2) and 0.15M NaCl
Preservative
0.01% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for MDR1/ABCB1 Antibody

  • ABC20
  • ABCB1
  • ABCB1B
  • ATP-binding cassette sub-family B member 1
  • ATP-binding cassette, sub-family B (MDR/TAP), member 1
  • CD243 antigen
  • CD243
  • CLCS
  • colchicin sensitivity
  • doxorubicin resistance
  • EC 3.6.3
  • EC 3.6.3.44
  • GP170
  • IBD13
  • MDR1
  • MDR1MGC163296
  • multidrug resistance protein 1
  • P-glycoprotein 1
  • P-gp
  • PGY1
  • PGY1P-GP

Background

P Glycoprotein, the product of the MDR1 gene, is expressed in distinct non-malignant cells, typically cells with secretory and excretory functions. It is assumed to function as an ATP-dependent drug efflux pump with broad substrate specificity. The highest expression of P Glycoprotein has been observed in kidney (proximal tubules), liver (bile canaliculi), adrenal gland and intestine, suggesting that the primary role of P Glycoprotein is in the normal secretion of physiological metabolites and ingested chemicals into bile, urine and the lumen of the intestinal tract. Elevated levels of P Glycoprotein have also been reported in multidrug-resistant cell lines and in colon, endometrial, ovarian, and breast tumors, as well as in sarcomas and leukemias / lymphomas.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunocytochemistry/Immunofluorescence: ABCG2 Antibody (3G8) [NBP2-22124] - Immunofluorescence analysis of Hela cells using ABCG2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
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Immunohistochemistry-Paraffin: MSH3 Antibody [NBP2-19417] - Immunohistochemical analysis of paraffin-embedded Colon carcinoma, using antibody at 1:500 dilution.
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Western Blot: MDMX Antibody [NB100-556] - Detection of Human HdmX/MDM4 by Western Blot. Samples: Whole cell lysate from Jurkat cells (50, 15, 5 ug) prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-HdmX/MDM4 antibody NB100-556 used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Western Blot: MDMX Antibody [NB100-556] - Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from Jurkat cells. Antibodies: Affinity purified rabbit anti-HdmX/MDM4 antibody NB100-556 (lot 2) used for WB at 1 ug/ml (A and B) and used for IP at 6 ug/mg lysate. HdmX/MDM4 was also immunoprecipitated by a previous lot (NB100-556 lot 1) of this antibody. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).
Western Blot: MDMX Antibody [NB100-556] - Analysis using the Biotin conjugate of NB100-556. Detection of Jurkat (50, 15, and 5 ug). Antibody used at 1.0 ug/ml. Detection: Streptavidin-HRP and chemiluminescence with an exposure time of 30 seconds.
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Western Blot: Cytochrome P450 3A4 Antibody (3H8) [NBP2-37502] - Western blot analysis using CYP3A4 mAb against HEK293 (1) and CYP3A4 (AA: 243-430)-hIgGFc transfected HEK293 (2) cell lysate.
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Immunocytochemistry/Immunofluorescence: ABCB6 Antibody [NBP2-58327] - Staining of human cell line U-251 MG shows localization to nucleoplasm & the Golgi apparatus.
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Immunohistochemistry-Paraffin: Cytochrome P450 3A5 Antibody [NBP2-29917] - Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with CYP3A5 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Western Blot: p53 Antibody (PAb 240) [NB200-103] - WB analysis of p53 in MCF7 and HeLa lystates. Image courtesy of anonymous customer product review.
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(3 Reviews)
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Immunohistochemistry-Paraffin: MRP3 Antibody [NBP2-37923] - Staining of human stomach shows strong membranous positivity in glandular cells.
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Immunocytochemistry/Immunofluorescence: LRP-1 Cluster III Antibody (SA0290) [NBP2-67286] - Staining LRP1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: LRP-1 Cluster III Antibody (SA0290) [NBP2-67286] - Analysis of paraffin-embedded mouse liver tissue using anti-LRP1 antibody. Counter stained with hematoxylin.
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Publications for MDR1/ABCB1 Antibody (NB100-80870) (0)

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Review for MDR1/ABCB1 Antibody (NB100-80870) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB100-80870:
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Western Blot MDR1/ABCB1 NB100-80870
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reviewed by:
Ewa Kaniewska
 WB Human 05/24/2014
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Summary

ApplicationWestern Blot
SpeciesHuman
Lot43176
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FAQs for MDR1/ABCB1 Antibody (NB100-80870). (Showing 1 - 1 of 1 FAQ).

  1. I'm wondering what advice you have in regards to storing antibodies in glycerol solution to prevent freeze thaw damage. I recently purchased an MDR1 antibody, rabbit host and polyclonal. The concentration is 1mg/ml.
    • Glycerol can indeed be added to the buffer for long term storage to help prevent freeze thaw cycle damage. Our lab recommends adding 30%.

Control Lysate(s)

MDR1/ABCB1 Overexpression Lysate (Native)

Secondary Antibodies

 

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Western Blot MDR1/ABCB1 NB100-80870
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 Ewa Kaniewska
05/24/2014
Application: WB
Species: Human
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Bioinformatics

Gene Symbol ABCB1
Entrez
Uniprot