MDR1/ABCB1 Antibody

Western Blot: MDR1/ABCB1 Antibody [NB100-80870] - Analyis of anti-ABCB1 antibody shows detection of ABCB1 in crude membrane extracts from HF insect cells over-expressing human ABCB1. The extract was loaded onto a gel in the amounts indicated followed by electrophoresis and transfer to nitrocellulose. The membrane was probed with the primary antibody diluted to 1:600, followed by Peroxidase Conjugated Affinity Purified Anti-RABBIT IgG at 1:10000. Personal Communication, Anna Calcagno, CCR-NCI, Bethesda, MD.

Immunohistochemistry-Paraffin: MDR1/ABCB1 Antibody [NB100-80870] - Staining of human kidney tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

• Reactivity: Hu, Mu, Rt, Ca, Pm
• Applications: WB, ELISA, IHC, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

MDR1/ABCB1 Antibody Summary

• Immunogen: Synthetic peptide from human MDR1/ABCB1.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ABCB1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:200 - 1:2000
  • ELISA 1:10000 - 1:50000
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
• Application Notes: IHC: NB100-80870 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity.
• Control:

  MDR1/ABCB1 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Buffer: 20mM Potassium Phosphate (pH 7.2) and 0.15M NaCl
• Preservative: 0.01% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for MDR1/ABCB1 Antibody

• ABC20
• ABCB1
• ABCB1B
• ATP-binding cassette sub-family B member 1
• ATP-binding cassette, sub-family B (MDR/TAP), member 1
• CD243 antigen
• CD243
• CLCS
• colchicin sensitivity
• doxorubicin resistance
• EC 3.6.3
• EC 3.6.3.44
• GP170
• IBD13
• MDR1
• MDR1MGC163296
• multidrug resistance protein 1
• P-glycoprotein 1
• P-gp
• PGY1
• PGY1P-GP

Background

P Glycoprotein, the product of the MDR1 gene, is expressed in distinct non-malignant cells, typically cells with secretory and excretory functions. It is assumed to function as an ATP-dependent drug efflux pump with broad substrate specificity. The highest expression of P Glycoprotein has been observed in kidney (proximal tubules), liver (bile canaliculi), adrenal gland and intestine, suggesting that the primary role of P Glycoprotein is in the normal secretion of physiological metabolites and ingested chemicals into bile, urine and the lumen of the intestinal tract. Elevated levels of P Glycoprotein have also been reported in multidrug-resistant cell lines and in colon, endometrial, ovarian, and breast tumors, as well as in sarcomas and leukemias / lymphomas.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MDR1/ABCB1 Antibody (NB100-80870)(2)

Publications using NB100-80870

• Pons V, LEvesque P, Plande M, Rivest S Conditional Genetic Deletion of CSF1 Receptor in Microglia Ameliorates the Physiopathology of Alzheimer s Disease Research Square Oct 6 2020 (WB, Mouse)
• Huang X, Zhuang J, Chung SW et al. Hypoxia-Tropic Protein Nanocages for Modulation of Tumor- and Chemotherapy-Associated Hypoxia. ACS Nano Dec 21 2018 [PMID: 30576113] (WB, Mouse)

Review for MDR1/ABCB1 Antibody (NB100-80870) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Ewa Kaniewska WB Human 05/24/2014

Summary

• Application: Western Blot
• Species: Human
• Lot: 43176
• File: View PDF

FAQs for MDR1/ABCB1 Antibody (NB100-80870). (Showing 1 - 1 of 1 FAQs).

1. I'm wondering what advice you have in regards to storing antibodies in glycerol solution to prevent freeze thaw damage. I recently purchased an MDR1 antibody, rabbit host and polyclonal. The concentration is 1mg/ml.
   • Glycerol can indeed be added to the buffer for long term storage to help prevent freeze thaw cycle damage. Our lab recommends adding 30%.

Bioinformatics

• Gene Symbol: ABCB1
• Entrez:
  • 5243(Human)
  • 18671(Mouse)
  • 24646(Rat)
• Uniprot:
  • P08183(Human)