Western Blot: MDR1/ABCB1 Antibody [NB100-80870] - Analyis of anti-ABCB1 antibody shows detection of ABCB1 in crude membrane extracts from HF insect cells over-expressing human ABCB1. The extract was loaded onto a gel in ...read more
Immunohistochemistry-Paraffin: MDR1/ABCB1 Antibody [NB100-80870] - Staining of human kidney tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
IHC: NB100-80870 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity.
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
20mM Potassium Phosphate (pH 7.2) and 0.15M NaCl
0.01% Sodium Azide
Immunogen affinity purified
Alternate Names for MDR1/ABCB1 Antibody
ATP-binding cassette sub-family B member 1
ATP-binding cassette, sub-family B (MDR/TAP), member 1
multidrug resistance protein 1
P Glycoprotein, the product of the MDR1 gene, is expressed in distinct non-malignant cells, typically cells with secretory and excretory functions. It is assumed to function as an ATP-dependent drug efflux pump with broad substrate specificity. The highest expression of P Glycoprotein has been observed in kidney (proximal tubules), liver (bile canaliculi), adrenal gland and intestine, suggesting that the primary role of P Glycoprotein is in the normal secretion of physiological metabolites and ingested chemicals into bile, urine and the lumen of the intestinal tract. Elevated levels of P Glycoprotein have also been reported in multidrug-resistant cell lines and in colon, endometrial, ovarian, and breast tumors, as well as in sarcomas and leukemias / lymphomas.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for MDR1/ABCB1 Antibody (NB100-80870). (Showing 1 - 1 of 1 FAQ).
I'm wondering what advice you have in regards to storing antibodies in glycerol solution to prevent freeze thaw damage. I recently purchased an MDR1 antibody, rabbit host and polyclonal. The concentration is 1mg/ml.
Glycerol can indeed be added to the buffer for long term storage to help prevent freeze thaw cycle damage. Our lab recommends adding 30%.
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