Home » MDR1/ABCB1 » MDR1/ABCB1 Antibodies » MDR1/ABCB1 Antibody

MDR1/ABCB1 Antibody

 (1 Review)
 See All Related
Submit a Review

Images

 
(
Enlarge)
Western Blot: MDR1/ABCB1 Antibody [NB100-80870] - Analyis of anti-ABCB1 antibody shows detection of ABCB1 in crude membrane extracts from HF insect cells over-expressing human ABCB1. The extract was loaded onto a gel in the amounts indicated followed by electrophoresis and transfer to nitrocellulose. The membrane was probed with the primary antibody diluted to 1:600, followed by Peroxidase Conjugated Affinity Purified Anti-RABBIT IgG at 1:10000. Personal Communication, Anna Calcagno, CCR-NCI, Bethesda, MD.
Western Blot: MDR1/ABCB1 Antibody [NB100-80870] - Analyis of anti-ABCB1 antibody shows detection of ABCB1 in crude membrane extracts from HF insect cells over-expressing human ABCB1. The extract was loaded onto a gel in ...read more
Immunohistochemistry-Paraffin: MDR1/ABCB1 Antibody [NB100-80870] - Staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, MkSpecies Glossary
Applications WB, ELISA, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

100% Guarantee on every product

MDR1/ABCB1 Antibody Summary

Immunogen
Synthetic peptide from human ABCB1 / P Glycoprotein.
Specificity
Amino acids 262-277 of human ABCB1 protein.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ABCB1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 1:200-1:2000
  • ELISA 1:10000-1:50000
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
Application Notes
IHC: NB100-80870 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity.
Positive Control
MDR1/ABCB1 Lysate (NBL1-07172)
Reviewed Applications
Read 1 Review rated 5
using
NB100-80870 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20mM Potassium Phosphate (pH 7.2) and 0.15M NaCl
Preservative
0.01% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for MDR1/ABCB1 Antibody

  • ABC20
  • ABCB1
  • ABCB1B
  • ATP-binding cassette sub-family B member 1
  • ATP-binding cassette, sub-family B (MDR/TAP), member 1
  • CD243 antigen
  • CD243
  • CLCS
  • colchicin sensitivity
  • doxorubicin resistance
  • EC 3.6.3
  • EC 3.6.3.44
  • GP170
  • IBD13
  • MDR1
  • MDR1MGC163296
  • multidrug resistance protein 1
  • P-glycoprotein 1
  • P-gp
  • PGY1
  • PGY1P-GP

Background

P Glycoprotein, the product of the MDR1 gene, is expressed in distinct non-malignant cells, typically cells with secretory and excretory functions. It is assumed to function as an ATP-dependent drug efflux pump with broad substrate specificity. The highest expression of P Glycoprotein has been observed in kidney (proximal tubules), liver (bile canaliculi), adrenal gland and intestine, suggesting that the primary role of P Glycoprotein is in the normal secretion of physiological metabolites and ingested chemicals into bile, urine and the lumen of the intestinal tract. Elevated levels of P Glycoprotein have also been reported in multidrug-resistant cell lines and in colon, endometrial, ovarian, and breast tumors, as well as in sarcomas and leukemias / lymphomas.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

MRP1 Antibody
(IU2H10)
NB400-156
Western Blot: MRP1 Antibody (IU2H10) [NB400-156] - Vector-transfected HEK293 cells 2: MRP1-transfected cells, prep1 3: MRP1-transfected cells, prep2 (the lower band is a degradation product.
Immunocytochemistry/Immunofluorescence: MRP1 Antibody (IU2H10) [NB400-156] - The MRP-1 antibody was tested in HeLa cells at a 1:50 dilution against Dylight 488 (Green). Alpha tubulin and nuclei were counterstained against Dylight 550 (Red) and DAPI (Blue), respectively.
(10 Publications)
Species: Hu, Mu, Rt
Applications: WB, ELISA, Flow, ICC/IF, IP, CyTOF-ready
ABCG2/CD338
Antibody (3G8)
NBP2-22124
Western Blot: ABCG2 Antibody (3G8) [NBP2-22124] - Analysis of ABCG2 expression in 1) human small intestine, 2) human placenta and 3) mouse placenta tissue extracts.
Immunocytochemistry/Immunofluorescence: ABCG2 Antibody (3G8) [NBP2-22124] - Immunofluorescence analysis of Hela cells using ABCG2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunohistochemistry-Paraffin: ABCG2 Antibody (3G8) [NBP2-22124] - Immunohistochemical analysis of paraffin-embedded bladder cancer tissues (left) and skeletal muscle tissues (right) using ABCG2 mouse mAb with DAB staining.
(2 Publications)
Species: Hu, Mu, Pm
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
MRP2 Antibody
(JA32-01)
NBP2-66958
Immunocytochemistry/Immunofluorescence: MRP2 Antibody (JA32-01) [NBP2-66958] - Staining MRP2 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: MRP2 Antibody (JA32-01) [NBP2-66958] - Analysis of paraffin-embedded human liver tissue using anti-MRP2 antibody. Counter stained with hematoxylin.
Flow Cytometry: MRP2 Antibody (JA32-01) [NBP2-66958] - Analysis of A549 cells with MRP2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P
MSH3 Antibody
NBP2-19417
Western Blot: MSH3 Antibody [NBP2-19417] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with MSH3 antibody diluted by 1:1000.
Immunocytochemistry/Immunofluorescence: MSH3 Antibody [NBP2-19417] - Analysis of paraformaldehyde-fixed HeLa, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: MSH3 Antibody [NBP2-19417] - Immunohistochemical analysis of paraffin-embedded Colon carcinoma, using antibody at 1:500 dilution.
(1 Publication)
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
MDMX Antibody
NB100-556
Western Blot: MDMX Antibody [NB100-556] - Detection of Human HdmX/MDM4 by Western Blot. Samples: Whole cell lysate from Jurkat cells (50, 15, 5 ug) prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-HdmX/MDM4 antibody NB100-556 used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Western Blot: MDMX Antibody [NB100-556] - Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from Jurkat cells. Antibodies: Affinity purified rabbit anti-HdmX/MDM4 antibody NB100-556 (lot 2) used for WB at 1 ug/ml (A and B) and used for IP at 6 ug/mg lysate. HdmX/MDM4 was also immunoprecipitated by a previous lot (NB100-556 lot 1) of this antibody. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).
Western Blot: MDMX Antibody [NB100-556] - Analysis using the Biotin conjugate of NB100-556. Detection of Jurkat (50, 15, and 5 ug). Antibody used at 1.0 ug/ml. Detection: Streptavidin-HRP and chemiluminescence with an exposure time of 30 seconds.
(32 Publications)
Species: Hu
Applications: WB, IP
Cytochrome P450
3A4 Antibody (3H8)
NBP2-37502
Western Blot: Cytochrome P450 3A4 Antibody (3H8) [NBP2-37502] - Western blot analysis using CYP3A4 mAb against HEK293 (1) and CYP3A4 (AA: 243-430)-hIgGFc transfected HEK293 (2) cell lysate.
Immunocytochemistry/Immunofluorescence: Cytochrome P450 3A4 Antibody (3H8) [NBP2-37502] - Immunofluorescence analysis of HepG2 cells using CYP3A4 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunohistochemistry: Cytochrome P450 3A4 Antibody (3H8) [NBP2-37502] - Immunohistochemical analysis of paraffin-embedded human brain tissues using CYP3A4 mouse mAb with DAB staining.
Species: Hu
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P, CyTOF-ready
ABCB6 Antibody
NBP2-58327
Western Blot: ABCB6 Antibody [NBP2-58327] - Western blot analysis in human cell line RT-4 and human cell line U-251 MG.
Immunocytochemistry/Immunofluorescence: ABCB6 Antibody [NBP2-58327] - Staining of human cell line U-251 MG shows localization to nucleoplasm & the Golgi apparatus.
Western Blot: ABCB6 Antibody [NBP2-58327] - Analysis in human cell lines Caco-2 and A-431. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-PARP1.
Species: Hu
Applications: WB, ICC/IF
MARCKS like
protein Antibody
NBP2-38160
Immunocytochemistry/Immunofluorescence: MARCKS like protein Antibody [NBP2-38160] - Immunofluorescent staining of human cell line Hep G2 shows localization to cytosol.
Immunohistochemistry: MARCKS like protein Antibody [NBP2-38160] - Staining of human lymph node shows strong cytoplasmic positivity in both germinal center cells and non-germinal center cells.
Species: Hu
Applications: ICC/IF, IHC, IHC-P
GPR177/WLS Antibody
NBP2-57299
Western Blot: GPR177/WLS Antibody [NBP2-57299] - Western blot analysis in human cell line RT-4, human cell line U-251 MG, human plasma, human liver tissue and human tonsil tissue.
Immunohistochemistry-Paraffin: GPR177/WLS Antibody [NBP2-57299] - Immunohistochemical staining of human placenta shows strong cytoplasmic positivity in cytotrophoblastic cells.
Species: Hu
Applications: WB, IHC, IHC-P
PGPEP-1 Antibody
AF6278
Western blot shows lysates of LNCaP human prostate cancer cell line, JAR human choriocarcinoma cell line, NTera‑2 human testicular embryonic carcinoma cell line, Neuro‑2A mouse neuroblastoma cell line, and human brain (cortex) tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human PGPEP‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6278) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for PGPEP‑1 at approximately 23 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Species: Hu, Mu
Applications: WB
MVP Antibody
NBP1-82820
Western Blot: MVP Antibody [NBP1-82820] - Analysis in human cell lines A-549 and HEK293. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-HDAC1.
Immunocytochemistry/Immunofluorescence: MVP Antibody [NBP1-82820] - Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: MVP Antibody [NBP1-82820] - Staining in human colon and pancreas tissues using anti-MVP antibody. Corresponding MVP RNA-seq data are presented for the same tissues.
Species: Hu
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
KLHL1 Antibody
NBP1-76670
Western Blot: KLHL1 Antibody [NBP1-76670] - Analysis of KLHL1 in HeLa cell lysate with KLHL1 antibody at (A) 2 and (B) 4 ug/mL.
Immunohistochemistry-Paraffin: KLHL1 Antibody [NBP1-76670] - Rat brain tissue with KLHL1 antibody at 10 ug/ml.
Western Blot: KLHL1 Antibody [NBP1-76670] - Analysis of KLHL1 in Human brain cell lysate with KLHL1 antibody using NBP1-76670 at (A) 2 and (B) 4 ug/ml.
Species: Hu, Rt
Applications: WB, ELISA, IHC, IHC-P
MDR3/ABCB4 Antibody
NBP2-30876
Immunohistochemistry-Paraffin: MDR3/ABCB4 Antibody [NBP2-30876] - Staining of human liver shows weak membranous positivity in bile duct cells.
Immunohistochemistry-Paraffin: MDR3/ABCB4 Antibody [NBP2-30876] - Staining of human liver shows strong positivity in bile canaliculi.
Immunohistochemistry-Paraffin: MDR3/ABCB4 Antibody [NBP2-30876] - Staining in human liver and skeletal muscle tissues.. Corresponding ABCB4 RNA-seq data are presented for the same tissues.
Species: Hu
Applications: IHC, IHC-P
PXR/NR1I2 Antibody
(1D12G1)
NBP2-61816
Western Blot: PXR/NR1I2 Antibody (1D12G1) [NBP2-61816] - Analysis using NR1I2 mAb against human NR1I2 (AA: 1-142) recombinant protein. (Expected MW is 42.2 kDa)
Immunocytochemistry/Immunofluorescence: PXR/NR1I2 Antibody (1D12G1) [NBP2-61816] - Analysis of Hela cells using NR1I2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Western Blot: PXR/NR1I2 Antibody (1D12G1) [NBP2-61816] - Analysis using NR1I2 mAb against HEK293 (1) and NR1I2 (AA: 1-142)-hIgGFc transfected HEK293 (2) cell lysate.
Species: Hu
Applications: WB, ELISA, Flow, ICC/IF, IHC
Cytochrome P450
3A5 Antibody
NBP2-29917
Western Blot: Cytochrome P450 3A5 Antibody [NBP2-29917] - Western blot analysis of anti-CYP3A5 Antibody (C-term) in 293 cell line lysates (35ug/lane). CYP3A5 (arrow) was detected using the purified Pab.
Immunocytochemistry/Immunofluorescence: Cytochrome P450 3A5 Antibody [NBP2-29917] - Confocal immunofluorescent analysis of CYP3A5 Antibody (C-term)(NBP2-29917) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain
Immunohistochemistry-Paraffin: Cytochrome P450 3A5 Antibody [NBP2-29917] - Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with CYP3A5 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Species: Hu
Applications: WB, ICC/IF, IHC-P
p53 Antibody (PAb
240)
NB200-103
Western Blot: p53 Antibody (PAb 240) [NB200-103] - WB analysis of p53 in MCF7 and HeLa lystates. Image courtesy of anonymous customer product review.
Immunocytochemistry/Immunofluorescence: p53 Antibody (PAb 240) [NB200-103] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-p53 (PAb 240) [NB200-103] at a 1:200 dilution overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunohistochemistry-Paraffin: p53 Antibody (PAb 240) [NB200-103] - p53 was detected in immersion fixed paraffin-embedded sections of human prostate cancer using anti-human mouse monoclonal antibody (Catalog # NB200-103) at 1:200 dilution overnight at 4C. Tissue was stained using the VisuCyte anti-mouse HRP polymer detection reagent (Catalog # VC001) with DAB chromogen (brown) and counterstained with hematoxylin (blue).Images may not be copied, printed or otherwise disseminated without express written permission of Novus Biologicals a bio-techne brand.
(2 Reviews)
(22 Publications)
Species: Hu, Mu, Rt, Ye, Xp(-)
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready, Flow-IC
MRP3 Antibody
NBP2-37923
Immunocytochemistry/Immunofluorescence: MRP3 Antibody [NBP2-37923] - Immunofluorescent staining of human cell line A549 shows localization to plasma membrane. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: MRP3 Antibody [NBP2-37923] - Staining of human stomach shows strong membranous positivity in glandular cells.
Immunohistochemistry-Paraffin: MRP3 Antibody [NBP2-37923] - Staining in human stomach and skeletal muscle tissues.. Corresponding ABCC3 RNA-seq data are presented for the same tissues.
Species: Hu
Applications: ICC/IF, IHC, IHC-P
LRP-1 Cluster III
Antibody (SA0290)
NBP2-67286
Western Blot: LRP-1 Cluster III Antibody (SA0290) [NBP2-67286] - Analysis of LRP1 on different lysates using anti-LRP1 antibody at 1/1,000 dilution. Positive control: Lane 1: Mouse liver Lane 2: Mouse brain Lane Lane 3: Mouse lung Lane 4: Human liver Lane 5: HepG2 Lane 6: Human lung
Immunocytochemistry/Immunofluorescence: LRP-1 Cluster III Antibody (SA0290) [NBP2-67286] - Staining LRP1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: LRP-1 Cluster III Antibody (SA0290) [NBP2-67286] - Analysis of paraffin-embedded mouse liver tissue using anti-LRP1 antibody. Counter stained with hematoxylin.
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP

Publications for MDR1/ABCB1 Antibody (NB100-80870) (0)

There are no publications for MDR1/ABCB1 Antibody (NB100-80870).
By submitting your publication information earn gift cards and discounts for future purchases.

Review for MDR1/ABCB1 Antibody (NB100-80870) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB100-80870:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot MDR1/ABCB1 NB100-80870
Enlarge
reviewed by:
Ewa Kaniewska
 WB Human 05/24/2014
View

Summary

ApplicationWestern Blot
SpeciesHuman
Lot43176
FileView PDF

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for MDR1/ABCB1 Antibody (NB100-80870). (Showing 1 - 1 of 1 FAQ).

  1. I'm wondering what advice you have in regards to storing antibodies in glycerol solution to prevent freeze thaw damage. I recently purchased an MDR1 antibody, rabbit host and polyclonal. The concentration is 1mg/ml.
    • Glycerol can indeed be added to the buffer for long term storage to help prevent freeze thaw cycle damage. Our lab recommends adding 30%.

Positive Control Lysate(s)

MDR1/ABCB1 Overexpression Lysate (Native)

Secondary Antibodies

 

Isotype Controls

Additional Array Products

Bioinformatics Tool for MDR1/ABCB1 Antibody (NB100-80870)

Discover related pathways, diseases and genes to MDR1/ABCB1 Antibody (NB100-80870). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for MDR1/ABCB1 Antibody (NB100-80870)

Discover more about diseases related to MDR1/ABCB1 Antibody (NB100-80870).
 

Pathways for MDR1/ABCB1 Antibody (NB100-80870)

View related products by pathway.

PTMs for MDR1/ABCB1 Antibody (NB100-80870)

Learn more about PTMs related to MDR1/ABCB1 Antibody (NB100-80870).
 

Research Areas for MDR1/ABCB1 Antibody (NB100-80870)

Find related products by research area.

Blogs on MDR1/ABCB1

There are no specific blogs for MDR1/ABCB1, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

  SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

5
5
1
4
0
3
0
2
0
1
0
Western Blot MDR1/ABCB1 NB100-80870
Enlarge
 Ewa Kaniewska
05/24/2014
Application: WB
Species: Human
read this review

Bioinformatics

Gene Symbol ABCB1
Entrez
Uniprot