MDR1/ABCB1 Antibody (MRK16) - Chimeric - Azide and BSA Free

Flow Cytometry: MDR1/ABCB1 Antibody (MRK16) - Chimeric [NBP2-52654] - Flow-cytometry using the MDR1/ABCB1 Antibody (MRK16) [NBP2-52654]. HepG2 cells were fixed using 2% PFA, permeabilised using 0.5% Triton and stained with anti-Fluorescein IgG antibody (isotype control, black line) or the rabbit IgG-chimeric version of MRK16 (NBP2-52654, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

• Reactivity: Hu
• Applications: Flow, ICC/IF, IP, CyTOF-ready
• Clone: MRK16
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

MDR1/ABCB1 Antibody (MRK16) - Chimeric - Azide and BSA Free Summary

• Description: Novus Biologicals Rabbit MDR1/ABCB1 Antibody (MRK16) - Chimeric - Azide and BSA Free (NBP2-52654) is a recombinant monoclonal antibody validated for use in Flow, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Drug resistant K-562 cells.
• Specificity: Recognizes a discontinuous extracellular epitope on P-glycoprotein.
• Isotype: IgG Kappa
• Clonality: Monoclonal
• Host: Rabbit
• Gene: ABCB1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:10 - 1:1000
  • Immunocytochemistry/ Immunofluorescence
  • Immunoprecipitation 1:10 - 1:500
• Application Notes: This antibody is Cytof ready. This chimeric rabbit antibody was made using the variable domain sequences of the original murine IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Packaging, Storage & Formulations

• Storage: Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
• Buffer: PBS
• Preservative: 0.02% Proclin 300
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for MDR1/ABCB1 Antibody (MRK16) - Chimeric - Azide and BSA Free

• ABC20
• ABCB1
• ABCB1B
• ATP-binding cassette sub-family B member 1
• ATP-binding cassette, sub-family B (MDR/TAP), member 1
• CD243 antigen
• CD243
• CLCS
• colchicin sensitivity
• doxorubicin resistance
• EC 3.6.3
• EC 3.6.3.44
• GP170
• IBD13
• MDR1
• MDR1MGC163296
• multidrug resistance protein 1
• P-glycoprotein 1
• P-gp
• PGY1
• PGY1P-GP

Background

MDR1 is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). MDR1 is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. The protein encoded by this gene is an ATP-dependent drug efflux pump for xenobiotic compounds with broad substrate specificity. It is responsible for decreased drug accumulation in multidrug-resistant cells and often mediates the development of resistance to anticancer drugs. This protein also functions as a transporter in the blood-brain barrier. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ABCB1