MD-1/LY86 Antibody - BSA Free

Western Blot: MD-1/LY86 Antibody [NBP1-76709] - Daudi cell lysate with LY86 antibody at (A) 1 and (B) 2 ug/mL.

Immunohistochemistry: MD-1/LY86 Antibody - BSA Free [NBP1-76709] - Immunohistochemistry of MD-1/LY86 in human spleen tissue with MD-1/LY86 antibody at 2.5 ug/mL.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

MD-1/LY86 Antibody - BSA Free Summary

• Immunogen: Antibody was raised against a 15 amino acid synthetic peptide from near the center of human MD-1. The immunogen is located within the last 50 amino acids of MD-1. Amino Acid Squence: CGRRKGEQIYYAGPV
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: LY86
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunohistochemistry 2.5 ug/ml
  • Immunohistochemistry-Paraffin 2.5 ug/ml
  • Western Blot 1-2 ug/ml
• Control Peptide:

  MD-1/LY86 Peptide (NBP1-76709PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for MD-1/LY86 Antibody - BSA Free

• LY86
• Ly-86
• lymphocyte antigen 86
• MD1
• MD-1
• MMD-1
• Protein MD-1
• RP105-associated

Background

Toll-like receptors (TLRs) are evolutionarily conserved pattern-recognition molecules that recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses. The signaling of these TLRs is kept under tight control by the expression of endogenous inhibiting proteins such as RP105, a recently identified homolog of TLR4. This protein, in association with MD-1, interacts with and inhibits the TLR4/MD-2 signaling pathway. While MD-2 can directly bind to LPS, one of the activating molecules of TLR4, the function of MD-1 is less well-known. It has been suggested however, that the RP105/MD-1 complex influences antibody production mediated by both TLR4/MD-2 and TLR2 receptor complexes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for MD-1/LY86 Antibody (NBP1-76709). (Showing 1 - 1 of 1 FAQ).

1. Have NBP1-75513 and NBP1-76709 been tested using immunoprecipitation techniques?
   • Unfortunately neither of these antibodies have been validated for IP. However, we do supply alternative products; NB100-56700 for instance is another LY86 antibody which has been tested for IP. Unfortunately none of our MD2 antibodies are validated for IP. If you would like to test any of these antibodies for an untested application/species, I recommend that you take advantage of our Innovator's Reward program.

Bioinformatics

• Gene Symbol: LY86
• Uniprot:
  • NP_004262(Human)