MCM7 Antibody - BSA Free

Western Blot: MCM7 Antibody [NB100-252] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified goat anti-MCM7 antibody used for WB at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Western Blot: MCM7 Antibody [NB100-252] - Whole cell lysate (60 ug) from HeLa cells. Antibody used at 0.25 ug/ml.

• Reactivity: Hu, Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

MCM7 Antibody - BSA Free Summary

• Description: Novus Biologicals Goat MCM7 Antibody - BSA Free (NB100-252) is a polyclonal antibody validated for use in WB. Anti-MCM7 Antibody: Cited in 5 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residues 300 and 350 of human Minichromosome Maintenance 7 using the numbering given in entry NP_005907.3 (GeneID 4176).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: MCM7
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:2000-1:10000
• Control:

  HeLa Whole Cell Lysate
  MCM7 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for MCM7 Antibody - BSA Free

• CDC47 homolog
• CDC47
• CDC47P85MCM
• DNA replication licensing factor MCM7
• EC 3.6.4.12
• homolog of S. cerevisiae Cdc47
• MCM2
• MCM2PNAS146
• MCM7 minichromosome maintenance deficient 7 (S. cerevisiae)
• MCM7
• minichromosome maintenance complex component 7
• minichromosome maintenance deficient (S. cerevisiae) 7
• minichromosome maintenance deficient 7
• P1.1-MCM3
• P1CDC47
• P85MCM
• PNAS146
• PPP1R104
• Protein Phosphatase 1

Background

The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 6 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. Cyclin D1-dependent kinase, CDK4, is found to associate with this protein, and may regulate the binding of this protein with the tumorsuppressor protein RB1/RB. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MCM7 Antibody (NB100-252)(5)

Publications using NB100-252

• Zhu Y, Hu S, Pan X et al. Hepatocyte Sirtuin 6 Protects against Atherosclerosis and Steatohepatitis by Regulating Lipid Homeostasis Cells 2023-08-05 [PMID: 37566087]
• Zhou HZ, Zeng HQ, Yuan D et al. NQO1 potentiates apoptosis evasion and upregulates XIAP via inhibiting proteasome-mediated degradation SIRT6 in hepatocellular carcinoma Cell Commun. Signal 2019-12-16 [PMID: 31842909]
• Tran L, Schulkin J, Ligon Co et al. Epigenetic modulation of chronic anxiety and pain by histone deacetylation. Mol. Psychiatry. 2014-10-07 [PMID: 25288139]
• Gurvich N, Perna F, Farina A et al. L3MBTL1 polycomb protein, a candidate tumor suppressor in del(20q12) myeloid disorders, is essential for genome stability. Proc Natl Acad Sci U S A 2010-12-01 [PMID: 21149733]
• Nash K, Chen W, Muzyczka N et al. Complete in vitro reconstitution of adeno-associated virus DNA replication requires the minichromosome maintenance complex proteins. J Virol 2008-02-01 [PMID: 18057257]

Bioinformatics

• Gene Symbol: MCM7
• Entrez:
  • 4176(Human)
• Uniprot:
  • P33993()