MCM7 Antibody

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Biological Strategies: Western Blot: MCM7 Antibody [NB100-252] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibodies: ...read more
Western Blot: MCM7 Antibody [NB100-252] - Whole cell lysate (60 ug) from HeLa cells. Antibody used at 0.25 ug/ml.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
1.0 mg/ml
Validated by:
   

Biological Strategies

     

Order Details

MCM7 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residues 300 and 350 of human Minichromosome Maintenance 7 using the numbering given in entry NP_005907.3 (GeneID 4176).
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
MCM7
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
Control
HeLa Lysate (NB800-PC1)
MCM7 Lysate (NBL1-12956)
Publications
Read Publications using NB100-252.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for MCM7 Antibody

  • CDC47 homolog
  • CDC47
  • CDC47P85MCM
  • DNA replication licensing factor MCM7
  • EC 3.6.4.12
  • homolog of S. cerevisiae Cdc47
  • MCM2
  • MCM2PNAS146
  • MCM7 minichromosome maintenance deficient 7 (S. cerevisiae)
  • MCM7
  • minichromosome maintenance complex component 7
  • minichromosome maintenance deficient (S. cerevisiae) 7
  • minichromosome maintenance deficient 7
  • P1.1-MCM3
  • P1CDC47
  • P85MCM
  • PNAS146
  • PPP1R104
  • Protein Phosphatase 1

Background

The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 6 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. Cyclin D1-dependent kinase, CDK4, is found to associate with this protein, and may regulate the binding of this protein with the tumorsuppressor protein RB1/RB. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MCM7 Antibody (NB100-252)(2)

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Control Lysate(s)

Secondary Antibodies

 

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Bioinformatics

Gene Symbol MCM7
Entrez
Uniprot