MCM6 Antibody (S02-2I2)

Western Blot: MCM6 Antibody (S02-2I2) [NBP3-19486] - Western blot detection of MCM6 in Jurkat cell lysates using NBP3-19486 (1:1000 diluted). Predicted band size: 93kDa. Observed band size: 93kDa.

Immunocytochemistry/Immunofluorescence: MCM6 Antibody (S02-2I2) [NBP3-19486] - Immunofluorescence of MCM6 (green) in Hela using NBP3-19486 at dilution 1/5, and DAPI (blue)

Immunohistochemistry-Paraffin: MCM6 Antibody (S02-2I2) [NBP3-19486] - Immunohistochemistry of MCM6 in paraffin-embedded Human tonsil using NBP3-19486 at dilution 1/50

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clone: S02-2I2
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.3 mg/ml

MCM6 Antibody (S02-2I2) Summary

• Description: Novus Biologicals Rabbit MCM6 Antibody (S02-2I2) (NBP3-19486) is a recombinant monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant protein of human MCM6 (Uniprot # Q14566)
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: MCM6
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1/50
  • Immunohistochemistry 1/500
  • Immunohistochemistry-Paraffin
  • Western Blot 1/1000
• Theoretical MW: 93 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA
• Preservative: 0.01% Sodium Azide
• Concentration: 0.3 mg/ml
• Purity: Affinity purified

Alternate Names for MCM6 Antibody (S02-2I2)

• DNA replication licensing factor MCM6
• EC 3.6.4.12
• MCG40308
• MCM6 minichromosome maintenance deficient 6 (MIS5 homolog, S. pombe) (S.cerevisiae)
• MCM6 minichromosome maintenance deficient 6 (MIS5 homolog, S. pombe)
• minichromosome maintenance complex component 6
• minichromosome maintenance deficient (mis5, S. pombe) 6
• minichromosome maintenance deficient 6 homolog (S. cerevisiae)
• minichromosome maintenance deficient 6 homolog
• MIS5 homolog
• Mis5
• p105MCM

Background

MCM6 is encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of the complex by CDC2 kinase reduces the helicase activity, suggesting a role in the regulation of DNA replication.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MCM6