MBP Antibody - BSA Free

Western Blot: MBP Antibody [NBP3-05553] - Western blot analysis of different tissue lysates using MBP Antibody at a dilution of 1:5,000 in green: [1] protein standard (red), [2] rat cerebellum, [3] mouse cerebellum, [4] cow midbrain and [5] pig midbrain. Multiple bands between 15-25 kDa mark correspond to the various alternate transcripts of the single MBP gene.

Immunocytochemistry/Immunofluorescence: MBP Antibody [NBP3-05553] - Immunofluorescent analysis of mouse hippocampus section stained with MBP Antibody at a dilution of 1:5,000 in red, and costained with rabbit pAb to GFAP at a dilution of 1:5,000 in green. The blue is Hoechst staining of nuclear DNA. The MBP antibody stains oligodendrocytes and myelin sheathes around axons, while the GFAP antibody reveals the network of glial cells.

Immunocytochemistry/Immunofluorescence: MBP Antibody [NBP3-05553] - Immunofluorescent analysis of mouse hippocampus section stained with MBP Antibody at a dilution of 1:5,000 in red, and costained with rabbit pAb to GFAP at a dilution of 1:5,000 in green. The blue is Hoechst staining of nuclear DNA. The MBP antibody stains oligodendrocytes and myelin sheathes around axons, while the GFAP antibody reveals the network of glial cells.

• Reactivity: Hu, Mu, Rt, Po, Bv
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

MBP Antibody - BSA Free Summary

• Immunogen: Purified MBP isolated from bovine brain
• Clonality: Polyclonal
• Host: Goat
• Gene: MBP
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:2000-5000
  • Western Blot 1:5000-10000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 50% PBS, 50% glycerol
• Preservative: 0.035% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for MBP Antibody - BSA Free

• Hmbpr
• MBP
• MGC99675
• mld
• Myelin A1 protein
• myelin basic protein
• Myelin membrane encephalitogenic protein
• shi

Background

Myelin Basic Protein (MBP) functions in the nervous system in myelination and is expressed in oligodendrocytes following differentiation. There are numerous isoforms generated by differential splicing events and post-translational modifications that have specialized functions. These functions include participation in signaling pathways prior to myelination, myelination or the re-myelination process following neural injury.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MBP