MBP Antibody (CL2819) - BSA Free

Western Blot: MBP Antibody (CL2819) [NBP2-46630] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Mouse Cerebral Cortex tissue

Immunocytochemistry/Immunofluorescence: MBP Antibody (CL2819) [NBP2-46630] - Staining of A549 cells using the Anti-MBP monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).

Immunohistochemistry: MBP Antibody (CL2819) [NBP2-46630] - Staining of human tonsil shows absence of immunoreactivity (negative control).

Western Blot: MBP Antibody (CL2819) [NBP2-46630] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Human Cerebral Cortex tissue

Immunohistochemistry: MBP Antibody (CL2819) [NBP2-46630] - Staining of rat cerebral cortex shows strong immunoreactivity in myelinated neural fibers.

Immunohistochemistry: MBP Antibody (CL2819) [NBP2-46630] - Staining of mouse cerebral cortex shows strong positivity in myelinated neural fibers.

Immunohistochemistry: MBP Antibody (CL2819) [NBP2-46630] - Staining of human cerebral cortex shows strong immunoreactivity in myelinated fibers.

Immunohistochemistry: MBP Antibody (CL2819) [NBP2-46630] - Staining of mouse striatum shows strong positivity in myelinated fibers.

Immunohistochemistry: MBP Antibody (CL2819) [NBP2-46630] - Staining of human cerebellum shows strong immunoreactivity in myelinated neural fibers.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clone: CL2819
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

MBP Antibody (CL2819) - BSA Free Summary

• Immunogen: This antibody was developed using a recombinant protein derived from P02686, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: MBP
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry 1:5000 - 1:10000
  • Immunohistochemistry-Paraffin 1:5000 - 1:10000
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. For ICC/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.
• Control Peptide:

  MBP Recombinant Protein Antigen (NBP2-46630PEP)

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Protein A purified

Alternate Names for MBP Antibody (CL2819) - BSA Free

• Hmbpr
• MBP
• MGC99675
• mld
• Myelin A1 protein
• myelin basic protein
• Myelin membrane encephalitogenic protein
• shi

Background

Myelin Basic Protein (MBP) functions in the nervous system in myelination and is expressed in oligodendrocytes following differentiation. There are numerous isoforms generated by differential splicing events and post-translational modifications that have specialized functions. These functions include participation in signaling pathways prior to myelination, myelination or the re-myelination process following neural injury.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MBP Antibody (NBP2-46630)(1)

Publication using NBP2-46630

• GrUter T, Blusch A, Motte J et al. Immunomodulatory and anti-oxidative effect of the direct TRPV1 receptor agonist capsaicin on Schwann cells J Neuroinflammation 2020-05-06 [PMID: 32375895]

Bioinformatics

• Gene Symbol: MBP
• Entrez:
  • 4155(Human)
• Uniprot:
  • P02686()