MBP Antibody (2H9) - BSA Free

Western Blot: MBP Antibody (2H9) [NBP2-22121] - Total human brain lysate was separated by SDS-PAGE on a 12% gel and transferred to PVDF membrane. The membrane was then probed with anti-MBP antibody at 2 ug/mL and detected using an anti-mouse HRP labeled secondary antibody and chemiluminescent substrate.

Immunocytochemistry/Immunofluorescence: Myelin Basic Protein Antibody (2H9) [NBP2-22121] - Immunofluorescence analysis of MSCS cells using Myelin Basic Protein mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Immunohistochemistry-Frozen: MBP Antibody (2H9) [NBP2-22121] - Mouse brain cryosections were stained with anti-MBP (1:400) and anti-mouse Alexa Fluor 555 IgG. Staining specific, hardly any background. Magnification 1:20. Image from verified customer review.

Flow Cytometry: Myelin Basic Protein Antibody (2H9) [NBP2-22121] - Flow cytometric analysis of HepG2 cells using Myelin Basic Protein mouse mAb (green) and negative control (red).

Immunohistochemistry-Paraffin: Myelin Basic Protein Antibody (2H9) [NBP2-22121] - Immunohistochemical analysis of paraffin-embedded brain tissues using Myelin Basic Protein mouse mAb with DAB staining.

Immunohistochemistry-Paraffin: Myelin Basic Protein Antibody (2H9) [NBP2-22121] - Immunohistochemical analysis of paraffin-embedded cerebellum tissues using Myelin Basic Protein mouse mAb with DAB staining.

ELISA: Myelin Basic Protein Antibody (2H9) [NBP2-22121] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng).

• Reactivity: Hu, Mu
• Applications: WB, ELISA, Flow, ICC/IF, IHC
• Clone: 2H9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

MBP Antibody (2H9) - BSA Free Summary

• Description: Novus Biologicals Mouse MBP Antibody (2H9) - BSA Free (NBP2-22121) is a monoclonal antibody validated for use in IHC, WB, ELISA, Flow and ICC/IF. Anti-MBP Antibody: Cited in 6 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Purified recombinant fragment of human Myelin Basic Protein expressed in E. coli. [UniProt# P02686]
• Marker: Oligodendrocyte Marker, Myelin Marker
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: MBP
• Purity: Ammonium sulfate precipitation

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Immunocytochemistry/ Immunofluorescence 1:200-1:1000
  • Immunohistochemistry
  • Immunohistochemistry-Frozen reported by customer review
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Western Blot 1:500-1:2000
• Theoretical MW: 33 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Mouse reactivity reported by customer review.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.03% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Ammonium sulfate precipitation

Alternate Names for MBP Antibody (2H9) - BSA Free

• Hmbpr
• MBP
• MGC99675
• mld
• Myelin A1 protein
• myelin basic protein
• Myelin membrane encephalitogenic protein
• shi

Background

The protein encoded by the classic MBP gene is a major constituent of the myelin sheath of oligodendrocytes and Schwann cells in the nervous system. However, MBP-related transcripts are also present in the bone marrow and the immune system. These mRNAs arise from the long MBP gene (otherwise called "Golli-MBP") that contains 3 additional exons located upstream of the classic MBP exons. Alternative splicing from the Golli and the MBP transcription start sites gives rise to 2 sets of MBP-related transcripts and gene products. The Golli mRNAs contain 3 exons unique to Golli-MBP, spliced in-frame to 1 or more MBP exons. They encode hybrid proteins that have N-terminal Golli aa sequence linked to MBP aa sequence. The second family of transcripts contain only MBP exons and produce the well characterized myelin basic proteins. This complex gene structure is conserved among species suggesting that the MBP transcription unit is an integral part of the Golli transcription unit and that this arrangement is important for the function and/or regulation of these genes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MBP Antibody (NBP2-22121)(6)

Publications using NBP2-22121

• Linnerbauer M, Beyer T, Nirschl L et al. PD-L1 positive astrocytes attenuate inflammatory functions of PD-1 positive microglia in models of autoimmune neuroinflammation Nat Commun 2023-09-09 [PMID: 37689786]
• Holloway RK, Zhang L, Molina-Gonzalez I et al. Localized microglia dysregulation impairs central nervous system myelination in development Acta neuropathologica communications 2023-03-22 [PMID: 36949514] (IHC-P, Human)
• Linnerbauer M, L��lein L, Farrenkopf D et al. Astrocyte-Derived Pleiotrophin Mitigates Late-Stage Autoimmune CNS Inflammation Frontiers in Immunology 2022-01-03 [PMID: 35046956] (In vivo assay)
• Ruhela D, Bhopale VM, Kalakonda S et al. Astrocyte-derived microparticles initiate a neuroinflammatory cycle due to carbon monoxide poisoning Brain Behav Immun Health 2021-12-01 [PMID: 34917988] (FLOW, Mouse)
• Mozafari S, Starost L, Manot-Saillet B, Garcia-Diaz B Multiple sclerosis iPSC-derived oligodendroglia conserve their intrinsic properties to functionally interact with axons and glia in vivo Sci Adv 2020-12-05 [PMID: 33277253]
• Schmitt C, Lechanteur A, Cossais F et al. Liposomal Encapsulated Curcumin Effectively Attenuates Neuroinflammatory and Reactive Astrogliosis Reactions in Glia Cells and Organotypic Brain Slices International Journal of Nanomedicine 2020-05-25 [PMID: 32547020] (IF/IHC, Mouse)

Reviews for MBP Antibody (NBP2-22121) (2)

Average Rating: 4.5

(Based on 2 reviews)

We have 2 reviews tested in 1 species: Mouse.

reviewed by: Verified Customer IHC-Fr Mouse 05/09/2019

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: Mouse brain
• Species: Mouse
• Lot: A1

reviewed by: Jan Martinek Immunofluorescence Frozen Acetone fixed Mouse 12/07/2018

Summary

• Application: Other
• Sample Tested: Mouse brain
• Species: Mouse
• Lot: A-1

FAQs for MBP Antibody (NBP2-22121). (Showing 1 - 1 of 1 FAQs).

1. Has this antibody been tested on mouse and rat tissues/cells to show exclusive staining to human cells?
   • For our product NBP2-22121, we have only tested this product in human samples thus far. We have not yet tested for cross reactivity with the mouse or rat proteins. The immunogen for this product shares 70% homology to the mouse sequence and 80% to the rat.

Bioinformatics

• Gene Symbol: MBP
• Entrez:
  • 4155(Human)
• Uniprot:
  • P02686()