MBL Antibody (3E7) - Azide Free

• Reactivity: Hu
• Applications: ELISA, Flow, Func, IHC
• Clone: 3E7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide Free
• Concentration: 0.1 mg/ml

MBL Antibody (3E7) - Azide Free Summary

• Immunogen: Serum isolated human RaRF
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: MBL2
• Purity: Protein G purified
• Endotoxin Note: <24 EU/mg

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry
  • Functional
  • Immunoassay
  • Immunohistochemistry-Frozen
• Application Notes: Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in user's experimental setting. Use in ELISA reported in scientific literature (PMID:35874468).

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 0.2 um filtered solution in PBS, 0.1% BSA
• Preservative: No Preservative
• Concentration: 0.1 mg/ml
• Purity: Protein G purified

Alternate Names for MBL Antibody (3E7) - Azide Free

• COLEC1
• COLEC1collectin-1
• Collectin-1
• HSMBPC
• Mannan-binding protein
• mannose-binding lectin (protein C) 2, soluble (opsonic defect)
• mannose-binding lectin (protein C) 2, soluble
• Mannose-binding lectin
• mannose-binding protein C
• MBL
• MBL2
• MBL2D
• MBLmannan-binding lectin
• MBP
• MBP1
• MBP1mannose-binding lectin 2, soluble (opsonic defect)
• MBP-C
• MGC116832
• MGC116833

Background

Mannose Binding Lectin (MBL) also called mannose- or mannan-binding protein (MBP) is a member of the group of collectins. MBL is an oligomeric lectin that recognizes carbohydrates as mannose and N-acetylglucosamine on pathogens. MBL contains a cysteine rich, a collagen like and a carbohydrate recognition domain. It forms a complex with C1r/C1s like serine proteases designated MASPs that proteolytically cleave C4, C2 and C3. MBL is able to activate the complement pathway independent of the classical and alternative complement activation pathways. The MBL-MASP pathway (better known as the lectin pathway) is antibody and C1q-independent. MBL exhibits complement-dependent antibacterial activity and acts directly as an opsonic and therefore plays an important role in innate immunity. MBL is synthesized by hepatocytes and has been isolated from the liver or serum of various vertebrate species.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MBL Antibody (NBP3-11223)(2)

Publications using NBP3-11223

• Hwang SY, Park S, Jo H et al. Interrupting specific hydrogen bonds between ELF3 and MED23 as an alternative drug resistance-free strategy for HER2-overexpressing cancers Journal of advanced research 2022-08-10 [PMID: 35963541] (WB, Human)
• Restrepo BN, Marin K, Romero P et al. Role of cytokines, chemokines, C3a, and mannose-binding lectin in the evolution of the chikungunya infection American journal of clinical and experimental immunology 2022-06-15 [PMID: 35874468] (ELISA, Human)

Bioinformatics

• Gene Symbol: MBL2