MBD3 Antibody (JB61-47)

Western Blot: MBD3 Antibody (JB61-47) [NBP2-75568] - Analysis of MBD3 on U937 cell lysate using anti-MBD3 antibody at 1/500 dilution.

Immunocytochemistry/Immunofluorescence: MBD3 Antibody (JB61-47) [NBP2-75568] - Staining MBD3 in NIH-3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: MBD3 Antibody (JB61-47) [NBP2-75568] - Analysis of paraffin-embedded rat testis tissue using anti-MBD3 antibody. Counter stained with hematoxylin.

Flow Cytometry: MBD3 Antibody (JB61-47) [NBP2-75568] - Analysis of SH-SY-5Y cells with MBD3 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: MBD3 Antibody (JB61-47) [NBP2-75568] - Staining MBD3 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: MBD3 Antibody (JB61-47) [NBP2-75568] - Staining MBD3 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: MBD3 Antibody (JB61-47) [NBP2-75568] - Analysis of paraffin-embedded human placenta tissue using anti-MBD3 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: MBD3 Antibody (JB61-47) [NBP2-75568] - Analysis of paraffin-embedded mouse brain tissue using anti-MBD3 antibody. Counter stained with hematoxylin.

Product Discontinued

MBD3 Antibody (JB61-47) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein within Human MBD3 aa 160-291 / 291. (SwissProt: O95983 Human; SwissProt: Q9Z2D8 Mouse; SwissProt: F7EY92 Rat)
• Localization: Chromosome, Nucleus.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: MBD3
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:500
  • Immunoprecipitation
  • Western Blot 1:500
• Theoretical MW: 33 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for MBD3 Antibody (JB61-47)

• MBD3
• methyl-CpG binding domain protein 3
• methyl-CpG-binding domain protein 3
• Methyl-CpG-binding protein MBD3

Background

DNA methylation, or the addition of methyl groups to cytosine bases in the dinucleotide CpG, is imperative to proper development and regulates gene expression. The methylation pattern involves the enzymatic processes of methylation and demethylation. The demethylation enzyme was recently found to be a mammalian protein, which exhibits demethylase activity associated to a methyl-CpG-binding domain (MBD) (4). The enzyme is able to revert methylated cytosine bases to cytosines within the particular dinucleotide sequence mdCpdG by catalyzing the cleaving of the methyl group as methanol. MeCP2 and MBD1 (PCM1) are first found to repress transcription by binding specifically to methylated DNA (3). MBD2 and MBD4 (also known as MED1) were later found to colocalize with foci of heavily methylated satellite DNA and believed to mediate the biological functions of the methylation signal. Surprisingly, MBD3 does not bind methylated DNA both in vivo and in vitro. MBD1, MBD2, MBD3, and MBD4 are found to be expressed in somatic tissues, but the expression of MBD1 and MBD2 is reduced or absent in embryonic stem cells, which are known to be deficient in MeCP1 activity. MBD4 have homology to bacterial base excision repair DNA N-glycosylases/lyases (2). In some microsatellite unstable tumors MBD4 is mutated at an exonic polynucleotide tract (1).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MBD3