MBD1 Antibody (100B272.1) - Azide and BSA Free

Images

 
Western Blot: MBD1 Antibody (100B272.1) - Azide and BSA Free [NBP2-33221] - Analysis of MBD1 in HeLa lysate using MBD1 antibody at 2 ug/ml (lane 1) and 1 ug/ml (lane 2).
Western Blot: MBD1 Antibody (100B272.1) - Azide and BSA Free [NBP2-33221] - Analysis of Jurkat cell lysate using anti-MBD1 antibody. Image from verified customer review.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ChIP, IP
Clone
100B272.1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
0.5 mg/ml

MBD1 Antibody (100B272.1) - Azide and BSA Free Summary

Immunogen
This antibody was generated by immunizing mice with a synthetic peptide corresponding to amino acids 391-405 (SESEDGAGSPPPYRR) of human MBD1; GenBank no ref|NP_056671.2|
Specificity
There are many isoforms of human MBD1 ranging from 503 (~ 55kD) to 655 (~ 71kD) amino acids in length. Researchers are encouraged to use the provided immunogen sequence to determine if this antibody is suitable for testing a specific isoform of interest.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
MBD1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2-4 ug/ml
  • Chromatin Immunoprecipitation 1:10-1:500
  • Immunoprecipitation
Application Notes
Use in chromatin immunoprecipitation reported in scientific literature (PMID 25798578). Use in Immunoprecipitation reported in scientific literature (PMID 15327775)

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 25798578)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS - BSA free
Preservative
No Preservative
Concentration
0.5 mg/ml
Purity
Protein G purified

Alternate Names for MBD1 Antibody (100B272.1) - Azide and BSA Free

  • CXXC-type zinc finger protein 3
  • methyl-CpG binding domain protein 1 isoform PCM1
  • methyl-CpG binding domain protein 1
  • methyl-CpG-binding domain protein 1
  • Methyl-CpG-binding protein MBD1
  • PCM1CXXC3RFT
  • Protein containing methyl-CpG-binding domain 1
  • the regulator of fibroblast growth factor 2 (FGF-2) transcription

Background

DNA methylation, or the addition of methyl groups to cytosine bases in the dinucleotide CpG, is imperative to proper development and regulates gene expression. The methylation pattern involves the enzymatic processes of methylation and demethylation. The demethylation enzyme was recently found to be a mammalian protein, which exhibits demethylase activity associated to a methyl-CpG-binding domain (MBD). The enzyme is able to revert methylated cytosine bases to cytosines within the particular dinucleotide sequence mdCpdG by catalyzing the cleaving of the methyl group as methanol. MeCP2 and MBD1 (PCM1) are first found to repress transcription by binding specifically to methylated DNA. MBD2 and MBD4 (also known as MED1) were later found to colocalize with foci of heavily methylated satellite DNA and believed to mediate the biological functions of the methylation signal. Surprisingly, MBD3 does not bind methylated DNA both in vivo and in vitro. MBD1, MBD2, MBD3, and MBD4 are found to be expressed in somatic tissues, but the expression of MBD1 and MBD2 is reduced or absent in embryonic stem cells, which are known to be deficient in MeCP1 activity. MBD4 have homology to bacterial base excision repair DNA N-glycosylases/lyases. In some microsatellite unstable tumors MBD4 is mutated at an exonic polynucleotide tract.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

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Bioinformatics

Gene Symbol MBD1
Entrez
Uniprot