Matrilin-1 Antibody (828806)

Matrilin-1 was detected in immersion fixed human mesenchymal stem cells differentiated into chondrocytes using Mouse Anti-Human Matrilin-1 Monoclonal Antibody (Catalog # MAB7878) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cartilage matrix. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu
• Applications: ICC/IF
• Clone: 828806
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Matrilin-1 Antibody (828806) Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human Matrilin-1
  Ser23-Val496
  Accession # P21941
• Specificity: Detects human Matrilin-1 in ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Matrilin-2, -3, or -4 is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: MATN1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Matrilin-1 Antibody (828806)

• cartilage matrix protein
• CMP
• CRTM
• MATN1
• matrilin 1, cartilage matrix protein
• Matrilin1
• Matrilin-1

Background

Matrilin-1, also known as Cartilage Matrix Protein (CMP) is a 50-60 kDa extracellular matrix protein that belongs to the superfamily of von Willebrand factor A (VWA) containing proteins. It is primarily expressed in cartilage and functions as a bridging component between proteins of the collagenous matrix. Mature human Matrilin-1 contains two VWA domains that flank one EGF-like repeat, followed by a C-terminal coiled coil domain. The Matrilins differ in the number of VWA domains (one or two) and EGF-like repeats (one, three, four, or ten) they contain. Human Matrilin-1 shares 91% aa sequence identity with human and rat Matrilin-1. The coiled coil domain of Matrilin-1 mediates disulfide-linked homo-trimer formation. It can also assemble into hetero-oligomers with Matrilin-2, -3, and -4.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MATN1
• Uniprot:
  • P21941(Human)