MASTL Antibody [Unconjugated]

MASTL was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human MASTL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF10022) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu
• Applications: ICC/IF
• Host: Goat
• Conjugate: Unconjugated

MASTL Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human MASTL.
  Lys39-Lys158
  Accession # Q96GX5
• Specificity: Detects human MASTL in direct ELISA.
• Source: N/A
• Isotype: IgG
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-15 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MASTL Antibody [Unconjugated]

• FLJ14813EC 2.7.11.1
• Greatwall
• GW
• GWL
• hGWL
• MASTL
• MAST-L
• microtubule associated serine/threonine kinase-like
• Microtubule-associated serine/threonine-protein kinase-like
• RP11-85G18.2
• THC2
• THC2greatwall protein kinase

Background

MAST-L is a ubiquitous microtubule associated serine/threonine kinase which plays a key role in mitosis. During entry into mitosis, CDK1 and MASTL kinase repress the activity of mitotic protein phosphatases PP1 and PP2A. Mitotic exit requires partial dephosphorylation of MASTL by PP1, followed by dephosphorylation of PP2A. PP1 is partially reactivated by decreases in CDK1 activity. PP1 then reactivates PP1A. Mutations of MASTL have been associated with autosomal dominant thrombocytopenia, a decrease in the number of platelets circulating in blood. MASTL kinase is frequently upregulated in several cancers, and is correlated with cancer progression, poor patient survival and tumor recurrence. Ectopic expression of MASTL in tumor cells has been shown to promote cell proliferation, and MASTL has been identified as a target for therapeutic intervention.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • Q96GX5()