Mas Antibody - BSA Free

Immunohistochemistry: Mas Antibody [NLS1531] - Analysis of MAS1 in mouse kidney using DAB with hematoxylin counterstain.

Immunohistochemistry-Paraffin: Mas Antibody [NLS1531] - Representative immunolocalisation images of AT1R, AT2R, MasR, and ACE2 in adult WKY rats and aged WKY rats. Mean data for aortic expression of the AT1R expressed as relative fluorescent units in adult (n = 5) and aged (n = 4) WKY rats. ***P < 0.001 versus adult WKY rats. Image collected and cropped by CiteAb from the following publication (//www.hindawi.com/journals/ijht/2012/192567/), licensed under a CC-BY license.

Immunohistochemistry-Paraffin: Mas Antibody [NLS1531] - Analaysis of Brain, Neurons and Glia using NLS1531 at 15 ug/ ml.

(a) Representative immunolocalisation images of AT1R, AT2R, MasR, and ACE2 in adult WKY rats and aged WKY rats. Mean data for aortic expression of the (b) AT1R, (c) AT2R, (d) MasR, and (e) ACE2 expressed as relative fluorescent units in adult (n = 5) and aged (n = 4) WKY rats. ***P < 0.001 versus adult WKY rats.

Immunocytochemistry/ Immunofluorescence: Mas Antibody - BSA Free [NLS1531] - (a) Representative immunolocalisation images of AT1R, AT2R, MasR, & ACE2 in adult WKY rats & aged WKY rats. Mean data for aortic expression of the (b) AT1R, (c) AT2R, (d) MasR, & (e) ACE2 expressed as relative fluorescent units in adult (n = 5) & aged (n = 4) WKY rats. ***P < 0.001 versus adult WKY rats. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/22187625), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: Mas Antibody - BSA Free [NLS1531] - Immunofluorescence studies in HEK293T cells overexpressing c-myc tagged MasR using MasR antibodies.Images of fluorescence signal corresponding to secondary cyanine (Cy3)-conjugated antibody (A), nuclear Hoechst 33258 staining (B), merged images (C) & bright field (D) are shown. In cells transfected with the pcDNA 3.1/c-myc-MasR construct, antibodies NLS-1531 & sc-54682 generated a similar staining pattern to that generated with the anti c-myc antibody. No staining was observed with any of these antibodies in cells transfected with the empty vector pcDNA 3.1. Antibody sc-135063 was capable of staining the plasma membrane of cells overexpressing the MasR but also generated widespread signals in cells transfected with the empty vector. For antibody AAR-013 there was no staining associated with the cell membrane. Intense immunocytochemical staining of identical distribution & intensity was revealed in cells transfected with the empty vector & those transfected with the c-myc-MasR construct. Images are representative of 3 independent experiments. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0183278), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunohistochemistry: Mas Antibody - BSA Free [NLS1531] - Immunohistochemistry in heart & kidney from wild-type (WT) & MasR-KO mice.Negative controls performed by omitting the primary antibody demonstrated minimal background immunostaining in heart (A) & kidney (B) sections. (A) In heart sections, NLS-1531 antibody stained predominantly the cytoplasm of cardiomyocytes with similar intensity in WT & MasR-KO hearts. The AAR-013 antibody stained the cardiomyocytes nucleus & weaker staining was observed in their cytoplasm. The same pattern was found in heart sections of MasR-KO mice. (B) In kidney sections, for NLS-1531 antibody, staining was mostly restricted to cytoplasm of numerous tubules cells with similar intensity in WT & MasR-KO kidneys. Antibody AAR-013 stained most intensely tubules cells nucleus & weaker staining was observed in their cytoplasm. Glomeruli were also stained positively. The same pattern was found in kidney sections of MasR-KO. Preincubation of the AAR-013 antibody with the blocking peptide provided by the vendors eliminated the immunohistochemical nuclear staining both in WT & MasR-KO mice kidney sections. Images are representative of n = 3. Bar, 50 μm. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0183278), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

• Reactivity: Hu, Mu, Rt
• Applications: Flow, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Mas Antibody - BSA Free Summary

• Immunogen: KLH conjugated synthetic peptide made to the C-terminus of human MAS1. [UniProt# P04201]
• Localization: Cell membrane
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MAS1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry reported in scientific literature (PMID 26386115)
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500. Use reported in scientific literature (PMID 22187625)
  • Immunohistochemistry 1:100-1:500
  • Immunohistochemistry-Frozen 1:100-1:500
  • Immunohistochemistry-Paraffin 1:100-1:500. Use reported in scientific literature (PMID 22187625)
• Application Notes: Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
• Control Peptide:

  Mas Protein (NLS1531PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.01% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Mas Antibody - BSA Free

• Mas
• MAS1 oncogene
• MAS1
• MGC119966
• proto-oncogene Mas

Background

MAS1 oncogene (Proto-oncogene Mas, MAS1) is a GTP binding protein coupled receptor which shares a conserved structural motif with other proteins of its class. When activated, MAS1 is a modulator of a critical step in the growth-regulating pathway to trigger oncogenes (PMID: 20674894). Evidence has shown that the MAS1 oncogene acts as a receptor for angiotensin (Ang)-(1-7), however it is not fully known how Intracellular signaling through Mas1 occurs (PMID: 19461648). Furthermore, research proves that in tandom with Ang-(1-7), the MAS1 receptor is expressed in the ovarian stroma of post menopausal women and in male testis (PMID: 20674894, 20361351). Additionally, together with Ang- (1-7) MAS1 may be an important component of normal spermatogenesis as low expression levels were found in infertile men with impaired spermatogenesis (PMID: 20361351).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Mas Antibody (NLS1531)(4)

Publications using NLS1531

• Burghi V, Fernandez NC, Gandola YB et al. Validation of commercial Mas receptor antibodies for utilization in Western Blotting, immunofluorescence and immunohistochemistry studies PLoS ONE 2017-08-16 [PMID: 28813513] (ICC/IF, Human)
• Singh N, Joshi S, Guo L et al. ACE2/Ang-(1-7)/Mas Axis Stimulates Vascular-Repair Relevant Functions of CD34+ Cells. Am. J. Physiol. Heart Circ. Physiol. 2015-09-18 [PMID: 26386115] (FLOW, Human)
• Jarajapu YP, Bhatwadekar AD, Caballero S et al. Activation of the ACE2/Angiotensin-(1-7)/Mas Receptor Axis Enhances the Reparative Function of Dysfunctional Diabetic Endothelial Progenitors Diabetes 2013-04-01 [PMID: 23230080]
• Bosnyak S, Widdop RE, Denton KM, Jones ES. Differential Mechanisms of Ang (1-7)-Mediated Vasodepressor Effect in Adult and Aged Candesartan-Treated Rats. International Journal of Hypertension 20122012:1-9. [PMID: 22187625]

Review for Mas Antibody (NLS1531) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer Flow Human 08/11/2016

Summary

• Application: Flow Cytometry
• Species: Human
• Lot: A

Bioinformatics

• Gene Symbol: MAS1
• Entrez:
  • 4142(Human)
• Uniprot:
  • P04201(Human)