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Mas Antibody

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 (1 Review)
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Immunohistochemistry: MAS1 Antibody [NLS1531] - IHC analysis of MAS1 in mouse kidney using DAB with hematoxylin counterstain.
Immunohistochemistry: MAS1 Antibody [NLS1531] - IHC analysis of MAS1 in mouse kidney using DAB with hematoxylin counterstain.
Immunohistochemistry-Paraffin: MAS1 Antibody [NLS1531] - IHC analysis of Brain, Neurons and Glia using NLS1531 at 15 ug/ ml.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications ICC/IF, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 405
NLS1531AF405
Alexa Fluor 488
NLS1531AF488
Alexa Fluor 647
NLS1531AF647
Alexa Fluor 700
NLS1531AF700
Biotin
NLS1531B
DyLight 405
NLS1531V
DyLight 488
NLS1531G
DyLight 550
NLS1531R
DyLight 650
NLS1531C
FITC
NLS1531F
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Mas Antibody Summary

Immunogen
KLH conjugated synthetic peptide made to the C-terminus of human MAS1. [UniProt# P04201]
Localization
Cell membrane
Clonality
Polyclonal
Host
Rabbit
Gene
MAS1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:100-1:500
  • Immunohistochemistry-Frozen 1:100-1:500
  • Immunohistochemistry-Paraffin 1:100-1:500
Application Notes
This MAS1 antibody is useful for Immunohistochemistry on paraffin-embedded sections and Immunocytochemistry/Immunofluorescence (PMID: 22187625). Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Reviewed Applications
Read 1 Review rated 5
using
NLS1531 in the following applications:
Publications
Read Publications using
NLS1531 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.7)
Preservative
0.01% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Mas Antibody

  • Mas
  • MAS1 oncogene
  • MAS1
  • MGC119966
  • proto-oncogene Mas

Background

MAS1 oncogene (Proto-oncogene Mas, MAS1) is a GTP binding protein coupled receptor which shares a conserved structural motif with other proteins of its class. When activated, MAS1 is a modulator of a critical step in the growth-regulating pathway to trigger oncogenes (PMID: 20674894). Evidence has shown that the MAS1 oncogene acts as a receptor for angiotensin (Ang)-(1-7), however it is not fully known how Intracellular signaling through Mas1 occurs (PMID: 19461648). Furthermore, research proves that in tandom with Ang-(1-7), the MAS1 receptor is expressed in the ovarian stroma of post menopausal women and in male testis (PMID: 20674894, 20361351). Additionally, together with Ang- (1-7) MAS1 may be an important component of normal spermatogenesis as low expression levels were found in infertile men with impaired spermatogenesis (PMID: 20361351).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunohistochemistry-Paraffin: Ceruloplasmin Antibody [NBP1-88116] - Staining of human liver shows moderate cytoplasmic positivity in hepatocytes.
(2 Publications)
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Applications: IHC, IHC-P
Serpin
A8/Angiotensinogen An
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Western Blot: Serpin A8/Angiotensinogen Antibody [NBP1-30027] - Analysis of Angiotensinogen in human kidney lysate.
Immunocytochemistry/Immunofluorescence: Serpin A8/Angiotensinogen Antibody [NBP1-30027] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-Angiotensinogen at 10 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunohistochemistry-Paraffin: Serpin A8/Angiotensinogen Antibody [NBP1-30027] - Analysis of Angiotensinogen in mouse kidney using DAB with hematoxylin counterstain.
(6 Publications)
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
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Western Blot: PMEL17/SILV Antibody [NBP1-69571] - Sample Tissue: Human MCF7 Antibody Dilution: 1.0 ug/ml
Immunohistochemistry: PMEL17/SILV Antibody [NBP1-69571] - Human kidney
Western Blot: PMEL17/SILV Antibody [NBP1-69571] - This Anti-SILV antibody was used in Western Blot of HepG2 tissue lysate at a concentration of 1.25ug/ml.
(4 Publications)
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Immunohistochemistry: SI Sucrase-Isomaltase Antibody [NBP1-87581] - Staining of human small intestine shows strong luminal membranous and cytoplasmic positivity in glandular cells.
(1 Publication)
Species: Hu
Applications: IHC, IHC-P
ACE-2 Antibody
[Unconjugated]
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Western blot shows lysates of human ovary tissue, human testis tissue, and human kidney tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human ACE‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF933) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for ACE‑2 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
ACE‑2 was detected in immersion fixed paraffin-embedded sections of human kidney using Goat Anti-Human ACE‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF933) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
ACE‑2 was detected in immersion fixed paraffin-embedded sections of human kidney using Goat Anti-Human ACE‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF933) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
(2 Reviews)
(17 Publications)
Species: Hu
Applications: WB, Simple Western, IHC, IP
ACE/CD143 Antibody
[Unconjugated]
AF1513
ACE/CD143 was detected in perfusion fixed frozen sections of mouse kidney using 15 µg/mL Goat Anti-Mouse ACE/CD143 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1513) overnight at 4 °C. Tissue was stained (red). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Western blot shows lysates of mouse lung tissue. PVDF membrane was probed with 0.05 µg/mL of Goat Anti-Mouse ACE/CD143 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1513) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for ACE/CD143 at approximately 180 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Simple Western lane view shows lysates of mouse lung tissue, loaded at 0.2 mg/mL. A specific band was detected for ACE/CD143 at approximately 218 kDa (as indicated) using 10 µg/mL of Goat Anti-Mouse ACE/CD143 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1513) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 66-440 kDa separation system.
(1 Review)
(3 Publications)
Species: Mu
Applications: WB, Simple Western, Flow, IHC, IP, CyTOF-ready
Placental
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NBP2-46376
Western Blot: Placental Lactogen/CSH1 Antibody (2G4) [NBP2-46376] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CSH1.
Immunohistochemistry: Placental Lactogen/CSH1 Antibody (2G4) [NBP2-46376] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: Placental Lactogen/CSH1 Antibody (2G4) [NBP2-46376] - Analysis of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Species: Hu
Applications: WB, IHC
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NBP2-45519
Western Blot: ERCC8 Antibody (2G1) [NBP2-45519] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ERCC8.
Immunohistochemistry: ERCC8 Antibody (2G1) [NBP2-45519] - Analysis of Human bladder tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: ERCC8 Antibody (2G1) [NBP2-45519] - Analysis of Carcinoma of Human thyroid tissue.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Species: Hu
Applications: WB, IHC

GRP75/HSPA9B/Mortalin
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Immunocytochemistry/Immunofluorescence: GRP75/HSPA9B/Mortalin Antibody (9F8) [NBP1-47801] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY GRP75/HSPA9B/Mortalin.
Immunohistochemistry-Paraffin: GRP75/HSPA9B/Mortalin Antibody (9F8) [NBP1-47801] - Staining of paraffin-embedded Human pancreas tissue using anti-GRP75/HSPA9B/Mortalin mouse monoclonal antibody.
Species: Hu, Mu, Rt, Ca, Mk, Pm
Applications: WB, Flow, ICC/IF, IHC, IHC-P
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NBP1-77078
Western Blot: AGTR-1 Antibody [NBP1-77078] - Mouse kidney tissue lysate with Angiotensin II Type 1 Receptor antibody at (A) 1 and (B) 2 ug/ml.
Immunocytochemistry/Immunofluorescence: AGTR-1 Antibody [NBP1-77078] - Mouse Kidney cells.
Immunohistochemistry: AGTR-1 Antibody [NBP1-77078] - Mouse kidney tissue at 2.5 ug/mL.
(5 Publications)
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
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Western Blot: Exosome component 10 Antibody [NBP1-32870] - analysis of EXOSC10 in HeLa whole cell extracts treated with (1) si-control and (2) EXOSC10 siRNA using anti-EXOSC10 antibody. Image from verified customer review.
Immunocytochemistry/Immunofluorescence: Exosome component 10 Antibody [NBP1-32870] - Analysis of paraformaldehyde-fixed A549, using Exosome component 10 antibody at 1:200 dilution.
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Western Blot: CS Citrate Synthase Antibody (1761) [NBP2-43648] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Citrate synthetase antibody [1761] diluted at 1:1000.
Immunocytochemistry/Immunofluorescence: CS Citrate Synthase Antibody (1761) [NBP2-43648] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Citrate synthetase protein stained by Citrate synthetase antibody [1761] diluted at 1:200. Red: phalloidin, a cytoskeleton marker, diluted at 1:50. Blue: Hoechst 33342 staining.
Immunohistochemistry-Paraffin: CS Citrate Synthase Antibody (1761) [NBP2-43648] - Analysis of mouse kidney. Citrate synthetase antibody [1761] dilution: 1:200.
(1 Review)
Species: Hu, Mu, Rt, Ze
Applications: WB, ICC/IF, IHC, IHC-P
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Western Blot: Coagulation Factor II/Thrombin Antibody [NBP1-58268] - 721_B tissue lysate at a concentration of 1ug/ml.
Immunohistochemistry: Coagulation Factor II/Thrombin Antibody [NBP1-58268] - Analysis of human liver after heat-induced Antigen retrieval. Antibody concentration 5 ug/ml.
Immunohistochemistry-Paraffin: Coagulation Factor II/Thrombin Antibody [NBP1-58268] - Human Placenta Tissue
(1 Review)
Species: Hu
Applications: WB, IHC, IHC-P
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Western Blot: Complement C4b/d Antibody (16D2) [NB200-541] - Western blot analysis of C4 expression in J774.A1 (A) aand mouse PBL (B) whole cell lysates.
(6 Publications)
Species: Mu
Applications: WB, ICC/IF, IHC, IHC-Fr, IP
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(1E8.)
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Western Blot: TTF2 Antibody (1E8) [H00008458-M06] - Analysis of TTF2 expression in NIH/3T3 (Cat # L018V1).
Immunocytochemistry/Immunofluorescence: TTF2 Antibody (1E8) [H00008458-M06] - Analysis of monoclonal antibody to TTF2 on HeLa cell. Antibody concentration 10 ug/ml
Western Blot: TTF2 Antibody (1E8) [H00008458-M06] - Analysis of TTF2 expression in PC-12 (Cat # L012V1).
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Western Blot: hnRNP C1 + C2 Antibody (CL2593) [NBP2-36776] - Lane 1: Marker [kDa]. Lane 2:Human cell line U-251 MG
Immunocytochemistry/Immunofluorescence: hnRNP C1 + C2 Antibody (CL2593) [NBP2-36776] - Staining in U251 cell line showing distinct nuclear (without nucleoli)Staining in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.
Immunohistochemistry: hnRNP C1 + C2 Antibody (CL2593) [NBP2-36776] - Staining in U2OS cell line with Anti-HNRNPC monoclonal antibody, showing distinct nuclear (without nucleoli) staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.
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Applications: WB, ICC/IF, IHC
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(N6-P2H5/G8)
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Western Blot: CYP51A1 Antibody (N6-P2H5-G8) [NBP2-50216] - Shows detection of a 60 kDa band.
Immunohistochemistry: CYP51A1 Antibody (N6-P2H5-G8) [NBP2-50216] - Staining of CYP51A1 in liver tissue.
Immunohistochemistry: CYP51A1 Antibody (N6-P2H5-G8) [NBP2-50216] - Photomicrograph of normal tissue.
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Publications for Mas Antibody (NLS1531)(3)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: FLOW.


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Review for Mas Antibody (NLS1531) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: Flow.

Reviews using NLS1531:
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Images Ratings Applications Species Date Details
Flow Cytometry Mas NLS1531
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reviewed by:
Anonymous
 Flow Human 08/11/2016
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Summary

ApplicationFlow Cytometry
SpeciesHuman
LotA
FileView PDF

Product General Protocols

View specific protocols for Mas Antibody (NLS1531): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

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ICC/IF Video Protocol

FAQs for Mas Antibody (NLS1531) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NLS1531AF405
Alexa Fluor 488 Labeled NLS1531AF488
Alexa Fluor 647 Labeled NLS1531AF647
Alexa Fluor 700 Labeled NLS1531AF700
Biotin Labeled NLS1531B
DyLight 405 Labeled NLS1531V
DyLight 488 Labeled NLS1531G
DyLight 550 Labeled NLS1531R
DyLight 650 Labeled NLS1531C
FITC Labeled NLS1531F
Learn about Custom Antibody Labeling

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Recent Reviews

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Flow Cytometry Mas NLS1531
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 Anonymous
08/11/2016
Application: Flow
Species: Human
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Bioinformatics

Gene Symbol MAS1
Entrez
Uniprot

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