Mas Antibody

Immunohistochemistry: Mas Antibody [NLS1531] - Analysis of MAS1 in mouse kidney using DAB with hematoxylin counterstain.

Immunohistochemistry-Paraffin: Mas Antibody [NLS1531] - Analaysis of Brain, Neurons and Glia using NLS1531 at 15 ug/ ml.

• Reactivity: Hu, Mu, Rt
• Applications: Flow, ICC/IF, IHC, IHC-Fr, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

Mas Antibody Summary

• Immunogen: KLH conjugated synthetic peptide made to the C-terminus of human MAS1. [UniProt# P04201]
• Localization: Cell membrane
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MAS1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:100-1:500
  • Immunohistochemistry-Frozen 1:100-1:500
  • Immunohistochemistry-Paraffin 1:100-1:500
• Application Notes: This MAS1 antibody is useful for Immunohistochemistry on paraffin-embedded sections and Immunocytochemistry/Immunofluorescence (PMID: 22187625). Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. Use in FLOW reported in scientific literature (PMID: 26386115).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.7)
• Preservative: 0.01% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Mas Antibody

• Mas
• MAS1 oncogene
• MAS1
• MGC119966
• proto-oncogene Mas

Background

MAS1 oncogene (Proto-oncogene Mas, MAS1) is a GTP binding protein coupled receptor which shares a conserved structural motif with other proteins of its class. When activated, MAS1 is a modulator of a critical step in the growth-regulating pathway to trigger oncogenes (PMID: 20674894). Evidence has shown that the MAS1 oncogene acts as a receptor for angiotensin (Ang)-(1-7), however it is not fully known how Intracellular signaling through Mas1 occurs (PMID: 19461648). Furthermore, research proves that in tandom with Ang-(1-7), the MAS1 receptor is expressed in the ovarian stroma of post menopausal women and in male testis (PMID: 20674894, 20361351). Additionally, together with Ang- (1-7) MAS1 may be an important component of normal spermatogenesis as low expression levels were found in infertile men with impaired spermatogenesis (PMID: 20361351).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Mas Antibody (NLS1531)(4)

Publications using NLS1531

• Burghi V, Fernandez NC, Gandola YB et al. Validation of commercial Mas receptor antibodies for utilization in Western Blotting, immunofluorescence and immunohistochemistry studies PLoS ONE 2017 Aug 16 [PMID: 28813513] (ICC/IF, Human)
• Singh N, Joshi S, Guo L et al. ACE2/Ang-(1-7)/Mas Axis Stimulates Vascular-Repair Relevant Functions of CD34+ Cells. Am. J. Physiol. Heart Circ. Physiol. 2015 Sep 18 [PMID: 26386115] (FLOW, Human)
• Jarajapu YP, Bhatwadekar AD, Caballero S et al. Activation of the ACE2/Angiotensin-(1-7)/Mas Receptor Axis Enhances the Reparative Function of Dysfunctional Diabetic Endothelial Progenitors Diabetes 2013 Apr [PMID: 23230080]
• Bosnyak S, Widdop RE, Denton KM, Jones ES. Differential Mechanisms of Ang (1-7)-Mediated Vasodepressor Effect in Adult and Aged Candesartan-Treated Rats. International Journal of Hypertension 20122012:1-9. [PMID: 22187625]

Review for Mas Antibody (NLS1531) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Anonymous Flow Human 08/11/2016

Summary

• Application: Flow Cytometry
• Species: Human
• Lot: A
• File: View PDF

Bioinformatics

• Gene Symbol: MAS1
• Entrez:
  • 4142(Human)
• Uniprot:
  • P04201(Human)