MARK2 Antibody (6O1C2)

Western Blot: MARK2 Antibody (6O1C2) [NBP3-16636] - Western blot analysis of extracts of various cell lines, using MARK2 Rabbit mAb (NBP3-16636) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: 6O1C2
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

MARK2 Antibody (6O1C2) Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 689-788 of human MARK2 (Q7KZI7). KPRSLRFTWSMKTTSSMEPNEMMREIRKVLDANSCQSELHEKYMLLCMHGTPGHEDFVQWEMEVCKLPRLSLNGVRFKRISGTSMAFKNIASKIANELKL
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: MARK2
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500 - 1:1000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol, 0.05% BSA
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for MARK2 Antibody (6O1C2)

• EC 2.7.11
• EC 2.7.11.1
• ELKL motif kinase 1
• ELKL motif kinase
• EMK-1
• EMK1PAR-1
• MAP/microtubule affinity-regulating kinase 2MGC99619
• PAR1 homolog
• Par1b
• Ser/Thr protein kinase PAR-1B
• serine/threonine protein kinase EMK
• serine/threonine-protein kinase MARK2

Background

EMK (ELKL Motif Kinase) is a small family of ser/thr protein kinases involved in the control of cell polarity, microtubule stability and cancer. Several cDNA clones have been isolated that encoded two isoforms of the human ser/thr protein kinase EMK1. These isoforms were characterized by the presence of a 162-bp alternative exon that gave rise to two forms, one containing the exon and the other one lacking it. Both forms were found to be coexpressed in a number of selected cell lines and tissue samples. The human EMK1 was shown to be encoded by a single mRNA ubiquitously expressed. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MARK2