MARCO Antibody [Biotin]

• Reactivity: Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

MARCO Antibody [Biotin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse MARCO
  Gln70-Ser518
  Accession # Q60754
• Specificity: Detects mouse MARCO in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: MARCO
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MARCO Antibody [Biotin]

• macrophage receptor with collagenous structureSCARA2Scavenger receptor class A member 2
• MARCO
• member 2
• SCARA2

Background

MARCO (macrophage receptor with collagenous structure), also known as SCARA2, is an 80 kDa type II transmembrane glycoprotein that belongs to the class A scavenger receptor family (1). Mouse MARCO consists of a 48 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 449 aa extracellular domain (ECD) that includes a stalk region, a collagen-like region, and one SRCR domain (2). Within the ECD, mouse MARCO shares 69% and 86% aa sequence identity with human and rat MARCO, respectively. It shares 18%‑28% aa sequence identity with other mouse class A scavenger receptors CL-P1, SCARA3, SCARA5, and SR-A1/MSR. MARCO is constitutively expressed on the surface of splenic and lymph node macrophages (2, 3). Its expression is induced on Kupffer cells and alveolar macrophages by microbial infection, chemical irritants, and Th1 polarizing factors (3‑5). MARCO binds LPS, lipoteichoic acid, and other determinants on Gram positive and Gram negative bacteria (2, 6‑8). It also binds modified LDL, CpG oligonucleotides, UGRP1, silica, and TiO₂ (2, 9‑11). MARCO is required for the organization of the splenic marginal zone and the interaction of local macrophages and B cells (12, 13). The SRCR domain mediates binding of MARCO to its various ligands (3, 12), while the collagen-like region mediates assembly into a disulfide-linked trimeric molecule (2, 7). MARCO ligation induces, but is not required for the production of IL-12, NO, or TNF-alpha by macrophages (5, 6, 9). MARCO knockout mice show a reduced clearance of bacterial infections, reduced mast cell mediated silicosis, increased pulmonary inflammation, and increased sensitivity to ozone induced lung damage (4, 9, 14‑16).

1. Murphy, J.E. et al. (2005) Atherosclerosis 182:1.
2. Elomaa, O. et al. (1995) Cell 80:603.
3. Van der Laan, L.J.W. et al. (1999) J. Immunol. 162:939.
4. Dahl, M. et al. (2007) J. Clin. Invest. 117:757.
5. Jozefowski, S. et al. (2005) J. Immunol. 175:8032. 
6. Mukhopadhyay, S. et al. (2006) Eur. J. Immunol. 36:940. 
7. Sankala, M. et al. (2002) J. Biol. Chem. 277:33378. 
8. Chen, Y. et al. (2006) J. Biol. Chem. 281:12767. 
9. Jozefowski, S. et al. (2006) J. Leukoc. Biol. 80:870.
10. Bin, L.-H. et al. (2003) J. Immunol. 171:924.
11. Hamilton, Jr. R.F. et al. (2006) J. Biol. Chem. 281:34218.
12. Karlsson, M.C.I. et al. (2003) J. Exp. Med. 198:333.
13. Chen, Y. et al. (2005) J. Immunol. 175:8173.
14. Arredouani, M. et al. (2004) J. Exp. Med. 200:267.
15. Arredouani, M.S. et al. (2007) J. Immunol. 178:5912.
16. Brown, J.M. et al. (2007) Am. J. Respir. Cell Mol. Biol. 36:43.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MARCO Antibody (BAF2956)(2)

Publications using BAF2956

• Abdouramane Camara, Alice C. Lavanant, Jun Abe, Henri Lee Desforges, Yannick O. Alexandre, Erika Girardi et al. CD169+ macrophages in lymph node and spleen critically depend on dual RANK and LTbetaR signaling Proceedings of the National Academy of Sciences 1/18/2022 [PMID: 35031565]
• CE McDougal, ZT Morrow, T Christophe, S Kim, D Carter, DM Stevenson, D Amador-Nog, MJ Miller, JD Sauer Phagocytes produce prostaglandin E2 in response to cytosolic Listeria monocytogenes PloS Pathogens, 2021-09-23;17(9):e1009493. 2021-09-23 [PMID: 34555127] (IHC, Mouse)

Bioinformatics

• Gene Symbol: MARCO
• Uniprot:
  • Q60754()