MAP2 Antibody (AP20)

Images

 
Immunocytochemistry/ Immunofluorescence: MAP2 Antibody (AP20) [NB600-1372] - Analysis of neurons using anti-MAP2 antibody followed by Alexa Fluor 488 goat anti-rabbit (Cat. No. A21206, green). Nuclear DNA was stained ...read more
Western Blot: MAP2 Antibody (AP20) [NB600-1372] - Rat brain cortex whole cell extracts were separated on SDS-PAGE, blotted with Monoclonal Anti-MAP2 (NB120-11268) and developed using Goat Anti-Mouse IgG, Alkaline ...read more
Immunocytochemistry/ Immunofluorescence: MAP2 Antibody (AP20) [NB600-1372] - DAPT, an inhibitor of Notch1 signaling, blocked the neuroprotective effects of melatonin against Abeta1-42 in hippocampal neurons. ...read more
Immunohistochemistry-Paraffin: MAP2 Antibody (AP20) [NB600-1372] - Human brain stained with Microtubule-Associated Protein 2a,b Ab-2 using peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of the neurons ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, Pgn, XpSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clone
AP20
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

MAP2 Antibody (AP20) Summary

Immunogen
MAP2 Antibody (AP20) was developed against bovine brain microtubule associated protein 2
Localization
Cytoskeleton
Marker
Neuronal Dendritic Marker
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
MAP2
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:20 - 50
  • Immunocytochemistry/Immunofluorescence 1:200
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:100 - 200
Theoretical MW
199 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NB600-1372 in the following applications:

Reactivity Notes

Pigeon reactivity reported in scientific literature (Braun E et al).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tissue culture supernatant
Preservative
0.09% Sodium Azide
Purity
Unpurified

Alternate Names for MAP2 Antibody (AP20)

  • DKFZp686I2148
  • MAP2
  • MAP-2
  • MAP2A
  • MAP2B
  • MAP2C
  • Microtubule Associated Protein 2
  • microtubule-associated protein 2
  • MTAP2

Background

Microtubule-associated protein 2 also known as MAP2 (theoretical molecular weight 280 kDa) belongs to the MAP2/Tau family which in addition to the two neuronal forms MAP2 and Tau also includes MAP4 (1). For MAP2, four different isoforms have been identified which are produced through alternative splicing including MAP2a, MAP2b, MAP2c and MAP2d (1). MAP2 isoforms contain multiple microtubule-binding domains located near the carboxy terminal, a variably sized amino-terminal projection domain, and a conserved protein kinase A binding domain. MAP2c and MAP2d isoforms have a shorter amino-terminal projection domain, lower molecular weight and are identifiable at ~70 kDa in SDS-PAGE analysis (2,3). MAP2's carboxy terminal domain supports interactions with microtubules, actin, and intermediate filaments (2).

MAP2 isoforms are developmentally regulated and differentially expressed in neurons and some glia. MAP2c is predominantly expressed in the developing brain while the other isoforms are expressed in the adult brain. The distribution of MAP2 isoforms also varies, with MAP2a and MAP2b predominantly localized to dendrites, while MAP2c is also found in axons. Lastly, the expression of MAP2d is not limited to neurons and may be found in glia, specifically oligodendrocytes (1, 2). MAP2 isoforms associate with microtubules and mediate their interaction with actin filaments thereby playing a critical role in organizing the microtubule-actin network. In neurons, MAP2 isoforms are implicated in different processes including neurite initiation, elongation and stabilization as well as axon and dendrite formation (2). Knockout of MAP expression in animal models results in a variety of functional and structural brain defects according to the isoform affected (e.g., reduced LTP and LTD, reduced myelination, absence of corpus collosum, motor system malfunction, abnormal hippocampal dendritic morphology, abnormal synaptic plasticity) (4).

References

1. Dehmelt, L., & Halpain, S. (2005). The MAP2/Tau family of microtubule-associated proteins. Genome Biology. https://doi.org/10.1186/gb-2004-6-1-204

2. Mohan, R., & John, A. (2015). Microtubule-associated proteins as direct crosslinkers of actin filaments and microtubules. IUBMB Life. https://doi.org/10.1002/iub.1384

3. Shafit-Zagardo, B., & Kalcheva, N. (1998). Making sense of the multiple MAP-2 transcripts and their role in the neuron. Molecular Neurobiology. https://doi.org/10.1007/BF02740642

4. Tortosa, E., Kapitein, L. C., & Hoogenraad, C. C. (2016). Microtubule organization and microtubule-associated proteins (MAPs). In Dendrites: Development and Disease. https://doi.org/10.1007/978-4-431-56050-0_3

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB300-141
Species: Hu, Mu, Rt, Po, Bv, Ch, Eq, Gp, Rb
Applications: WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P
NB100-82245
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, IHC-P, KD
NBP2-13304
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NB300-653
Species: Hu, Mu, Rt, Ch, Fe, Pm
Applications: WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, IP
MAB1259
Species: Hu
Applications: CyTOF-reported, ICC, ICFlow
NBP1-42827
Species: Hu, Mu, Rt, Ch, Fi, Re, Xp
Applications: WB, ICC/IF, IHC, IHC-Fr
NBP1-88191
Species: Hu, Rt
Applications: WB, IHC, IHC-P
H00064112-M02
Species: Hu
Applications: WB, ELISA, ICC/IF, S-ELISA
NB600-717
Species: Hu, Mu, Rt, Po, Bv, Ca, Ch, Fe, Gp, Ma, Rb, Sh
Applications: WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, RI
NB110-58870
Species: Hu, Mu, Rt
Applications: WB, Simple Western, ICC/IF, IHC, KD
NB300-223
Species: Hu, Mu, Rt, Po, Bv, Ca, Ch, Eq
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
NBP2-14871
Species: Hu, Mu, Rt
Applications: WB, ICC/IF
NBP2-22203
Species: Hu, Mu, Rt, Pm
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
NB300-109
Species: Hu, Mu, Rt, Dr, I, Ma
Applications: WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P, Dual ISH-IHC, IHC-FrFl, IHC-WhMt, KO

Publications for MAP2 Antibody (NB600-1372)(10)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Pigeon.

We have publications tested in 5 applications: ICC/IF, IHC, IHC-FrFl, IHC-P, WB.


Filter By Application
ICC/IF
(2)
IHC
(3)
IHC-FrFl
(1)
IHC-P
(1)
WB
(2)
All Applications
Filter By Species
Human
(2)
Mouse
(2)
Rat
(4)
Pigeon
(1)
All Species
Showing Publications 1 - 10 of 10.
Publications using NB600-1372 Applications Species
Bronzuoli MR, Facchinetti R, Steardo L et al. Palmitoylethanolamide Dampens Reactive Astrogliosis and Improves Neuronal Trophic Support in a Triple Transgenic Model of Alzheimer's Disease:In VitroandIn VivoEvidence. Oxid Med Cell Longev. Jan 16 2018 [PMID: 29576849] (ICC/IF, Human) ICC/IF Human
Scuderi C, Bronzuoli MR, Facchinetti R, Pace L. Ultramicronized palmitoylethanolamide rescues learning and memory impairments in a triple transgenic mouse model of Alzheimer's disease by exerting anti-inflammatory and neuroprotective effects. Transl Psychiatry. 2018 Jan 31 [PMID: 29382825] (IHC, Mouse) IHC Mouse
Hu C, Wang P, Zhang S et al. Neuroprotective effect of melatonin on soluble AB1-42-induced cortical neurodegeneration via Reelin-Dab1 signaling pathway. Neurol. Res. Apr 7 2017 [PMID: 28385118] (ICC/IF, Rat) ICC/IF Rat
Zhang S, Wang P, Ren L et al. Protective effect of melatonin on soluble Abeta1-42-induced memory impairment, astrogliosis, and synaptic dysfunction via the Musashi1/Notch1/Hes1 signaling pathway in the rat hippocampus. Alzheimers Res Ther Sep 16 2016 [PMID: 27630117] (IHC, Rat) IHC Rat
Heitz FD, Farinelli M, Mohanna S et al. The memory gene KIBRA is a bidirectional regulator of synaptic and structural plasticity in the adult brain. Neurobiol Learn Mem. Aug 3 2016 [PMID: 27498008] (IHC, Mouse) IHC Mouse
Kong HK, Wong MH, Chan HM, Lo SC. Chronic Exposure of Adult Rats to Low Doses of Methylmercury Induced a State of Metabolic Deficit in the Somatosensory Cortex. J Proteome Res. 2013 Aug 28 [PMID: 23984759] (WB, Rat) WB Rat
Sakurai A, Makioka K, Fukuda T et al. Accumulation of phosphorylated TDP-43 in the CNS of a patient with Cockayne syndrome. Neuropathology 2013 Apr 14 [PMID: 23581709] (IHC-P, Human) IHC-P Human
Kalcheva N et al. Localization of specific epitopes on human microtubule-associated protein 2. J Neurochem 63:2336-41. 1994 [PMID: 7525876]
Braun E, Hotter D, Koepke L et al. A comparative assessment of aging-related NADPH diaphorase positivity in the spinal cord and medullary oblongata between pigeon and murine bioRxiv (IHC-FrFl, Pigeon) IHC-FrFl Pigeon
Maftei D, Ratano P, Fusco I et al. The prokineticin receptor antagonist PC1 rescues memory impairment induced by beta amyloid administration through the modulation of prokineticin system Neuropharmacology Aug 10 2019 [PMID: 31408628] (WB, Rat) WB Rat

Reviews for MAP2 Antibody (NB600-1372) (0)

There are no reviews for MAP2 Antibody (NB600-1372). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for MAP2 Antibody (NB600-1372) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Additional MAP2 Products

Bioinformatics Tool for MAP2 Antibody (NB600-1372)

Discover related pathways, diseases and genes to MAP2 Antibody (NB600-1372). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for MAP2 Antibody (NB600-1372)

Discover more about diseases related to MAP2 Antibody (NB600-1372).
 

Pathways for MAP2 Antibody (NB600-1372)

View related products by pathway.

PTMs for MAP2 Antibody (NB600-1372)

Learn more about PTMs related to MAP2 Antibody (NB600-1372).
 

Research Areas for MAP2 Antibody (NB600-1372)

Find related products by research area.

Blogs on MAP2.

Methamphetamine with HIV induces mitochondrial dysfunction and neuronal injury through oxidative stress
By Jamshed Arslan, Pharm. D., PhD. December 1 is the World AIDS Day. Despite the combination antiretroviral therapy, 10-25% of Human Immunodeficiency Virus (HIV)-positive individuals report neurocognitive impairm...  Read full blog post.

Deriving neural precursor cells from human induced pluripotent stem cells
By Jennifer Sokolowski, MD, PhD.Human induced pluripotent stem cells (iPSCs) can be used to create models of human organ systems and are useful for a) ascertaining the mechanisms underlying pathological conditions...  Read full blog post.


  Read full blog post.

Beta Tubulin III and neurogenesis
Beta tubulin III, also known as Tuj-1, is a class III member of the beta tubulin protein family. Beta tubulins are one of two structural components that form our microtubule network. While general tubulins play a role in a wide range of cellular pr...  Read full blog post.

Synapsin I: Implicated in synaptic activity across a diverse range of studies
Synapsins are a family of neuronal proteins that are most renowned for their activity in modulating the pre-synaptic terminal.  Synapsin’s behavior is regulated by protein kinases and phosphatases, which alter the way that synapsin’s i...  Read full blog post.

Coronavirus Brochure

Customers Who Bought This Also Bought

Contact Information

Learn the difference between western blot and simple western

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our MAP2 Antibody (AP20) and receive a gift card or discount.

Bioinformatics

Gene Symbol MAP2
Entrez