MAP2 Antibody (885232) [Unconjugated]

MAP2 was detected in immersion fixed paraffin-embedded sections of human Brain Cortex using Mouse Anti-Human MAP2, Monoclonal Antibody (Catalog # MAB8304) at 20ug/mL at 37 ° Celsius for 8 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cytoplasm. Protocol available in COMET™ Panel Builder.​

MAP2 was detected in immersion fixed human embryonic stem cells/neurospheres using Mouse Anti-Human MAP2 Monoclonal Antibody (Catalog # MAB8304) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

MAP2 was detected in immersion fixed paraffin-embedded sections of human Alzheimer's brain using Mouse Anti-Human MAP2 Monoclonal Antibody (Catalog # MAB8304) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to neurofibrillary tangles and plaques. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: IHC, ICC/IF, mIF
• Clone: 885232
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

MAP2 Antibody (885232) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human MAP2
  Gly1689-Lys1824
  Accession # P11137
• Specificity: Detects human MAP2 in direct ELISAs.
• Source: N/A
• Isotype: IgG3
• Clonality: Monoclonal
• Host: Mouse
• Gene: MAP2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 0.5-25 ug/mL
  • Immunohistochemistry 0.5-25 ug/mL
  • Multiplex Immunofluorescence 20 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in TBS with Trehalose. See Certificate of Analysis for details.
  *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MAP2 Antibody (885232) [Unconjugated]

• DKFZp686I2148
• MAP2
• MAP-2
• MAP2A
• MAP2B
• MAP2C
• Microtubule Associated Protein 2
• microtubule-associated protein 2
• MTAP2

Background

MAP2 (Microtubule-associated protein 2) is a 1827 amino acid (aa) cytoskeletal associated protein.  Human MAP2 shares 84% and 79% aa identity with mouse and rat MAP2, respectively.  Multiple splice forms exist, resulting in 4 distinct isoforms.  MAP2 functions in microtubule polymerization and stabilization in both normal and malignant cell types.  It has been demonstrated to play a critical role in neurite outgrowth and loss of MAP2 function in neurons may lead to neural degeneration.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MAP2 Antibody (MAB8304)(5)

Publications using MAB8304

• Kuroda, T;Yasuda, S;Matsuyama, S;Miura, T;Sawada, R;Matsuyama, A;Yamamoto, Y;Morioka, MS;Kawaji, H;Kasukawa, T;Itoh, M;Akutsu, H;Kawai, J;Sato, Y; ROR2 expression predicts human induced pluripotent stem cell differentiation into neural stem/progenitor cells and GABAergic neurons Scientific reports 2024-01-06 [PMID: 38184695] (Immunocytochemistry, Human)
• Rallapalli, H;Bayin, NS;Goldman, H;Maric, D;Nieman, BJ;Koretsky, AP;Joyner, AL;Turnbull, DH; Cell Specificity of Manganese-enhanced MRI Signal in the Cerebellum NeuroImage 2023-05-26 [PMID: 37245561] (IHC, Mouse)
• Andrew K. Ressler, Gabriela L.A. Sampaio, Sarah A. Dugger, Tamar Sapir, Daniel Krizay, Michael J. Boland et al. Evidence of shared transcriptomic dysregulation of HNRNPU-related disorder between human organoids and embryonic mice iScience 1/20/2023 [PMID: 36594023]
• Mouhamed Alsaqati, Brittany A. Davis, Jamie Wood, Megan M. Jones, Lora Jones, Aishah Westwood et al. NRSF/REST lies at the intersection between epigenetic regulation, miRNA-mediated gene control and neurodevelopmental pathways associated with Intellectual disability (ID) and Schizophrenia Translational Psychiatry 10/10/2022 [PMID: 36216811]
• Aditya Rayasam, Julie A. Kijak, Lee Kissel, Yun Hwa Choi, Taehee Kim, Martin Hsu et al. CXCL13 expressed on inflamed cerebral blood vessels recruit IL-21 producing TFH cells to damage neurons following stroke Journal of Neuroinflammation 12/1/2022 [PMID: 35624463]

Bioinformatics

• Gene Symbol: MAP2
• Uniprot:
  • P11137()