Recombinant Human MAGP-1/MFAP2 His Protein

SDS-Page: Recombinant Human MAGP-1/MFAP2 Protein [NBP2-23234]

• Reactivity: Hu
• Applications: PAGE
• Concentration: 0.5 mg/ml

Recombinant Human MAGP-1/MFAP2 His Protein Summary

• Description: A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 18-183 of Human MAGP-1/MFAP2
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMQGQYDL DPLPPFPDHV QYTHYSDQID NPDYYDYQEV TPRPSEEQFQ FQSQQQVQQE VIPAPTPEPG NAELEPTEPG PLDCREEQYP CTRLYSIHRP CKQCLNEVCF YSLRRVYVIN KEICVRTVCA HEELLRADLC RDKFSKCGVM ASSGLCQSVA ASCARSCGSC

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: MFAP2
• Purity: >85%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Application Notes: Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays.
• Theoretical MW: 21.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 1 M UREA, 20% glycerol
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: >85%, by SDS-PAGE

Alternate Names for Recombinant Human MAGP-1/MFAP2 His Protein

• MAGP1
• MAGP-1
• MAGP-1FLJ50901
• MAGPMFAP-2
• MFAP2
• Microfibril-associated glycoprotein 1
• microfibrillar-associated protein 2

Background

Microfibrillar-associated protein 2, also known as MFAP2, is an O-glycosylated protein secreted to the extracellular space and the extracellular matrix. MFAP2 associates with biglycan and elastin in a ternary complex. It is shown to play a significant role in the support and distensibility of the juxtacanalicular region of these collector channels. It is also reported to inhibit LTB-1 binding to fibrillin-1, stimulate the phosphorylation of Smad2, and thereby mediate the subsequent extracellular deposition of latent TGFbeta. Recombinant human MFAP2, fused to His-tag at N-terminus, was expressed in E.coli.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: MFAP2