MAG/Siglec-4a Antibody (EP971Y)

Product Discontinued

MAG/Siglec-4a Antibody (EP971Y) Summary

• Immunogen: A synthetic peptide corresponding to residues near the N-terminus of human MAG was used as an Immunogen.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: MAG
• Purity: Tissue culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:20
  • Immunoprecipitation 1:50
  • Western Blot 1:500-5000
• Application Notes: In Western blot this antibody detects a band at approximately 100 kDa.

Reactivity Notes

Cross reactivity determined by Western Blot only.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 49% PBS, 0.05% BSA and 50% Glycerol
• Preservative: 0.01% Sodium Azide
• Purity: Tissue culture supernatant

Notes

Produced using Abcam's RabMab® technology. RabMab® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Alternate Names for MAG/Siglec-4a Antibody (EP971Y)

• GMAsialic acid binding Ig-like lectin 4A
• MAG
• myelin associated glycoprotein
• myelin-associated glycoprotein
• sialic acid-binding immunoglobulin-like lectin 4A
• Siglec4a
• Siglec-4a
• S-MAG

Background

Myelin-associated glycoprotein (MAG), a membrane glycoprotein of 100 kDa, is thought to be involved in the process of myelination (1). Axonal regeneration in the adult central nervous system (CNS) is limited by two proteins in myelin, Nogo and MAG (2). MAG has been implicated in inhibition of nerve regeneration in the CNS. This results from interactions between MAG and the Nogo receptor and gangliosides on the apposing axon, which generates intracellular inhibitory signals in the neuron (3). Results also suggest that MAG binds to a specific receptor and initiates a signal transduction cascade to effect inhibition. These results indicate that soluble dMAG detected in vivo could contribute to the lack of regeneration in the mammalian CNS after injury (4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for MAG/Siglec-4a Antibody (NB110-57177) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Sandra Canosa WB Mouse 06/15/2010

Summary

• Application: Western Blot
• Sample Tested: Mouse neural stem cells lysate and whole embryo (E8.5) homogenate, Sample Amount: 15 ug
• Species: Mouse
• Lot: YD070601

Bioinformatics

• Gene Symbol: MAG
• Entrez:
  • 4099(Human)
  • 17136(Mouse)
  • 29409(Rat)
• Uniprot:
  • P20916()