MAD4 Antibody

Western Blot: MAD4 Antibody [NB100-1117] - (0.5 ug/ml) of human kidney lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence

• Reactivity: Hu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

MAD4 Antibody Summary

• Description: Novus Biologicals Goat MAD4 Antibody (NB100-1117) is a polyclonal antibody validated for use in WB and ELISA. Anti-MAD4 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence GPHCRRLGRPALS corresponding to C-Terminus according to NP_006445.1.
• Localization: Nuclear
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: MXD4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 0.5 - 2 ug/ml
• Application Notes: WB: Approx. 22 kDa band observed in human kidney lysates (predicted MW of 24 kDa band according to NP_006445). Please note the appearance of this band was blocked by incubation with the immunizing peptide.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for MAD4 Antibody

• BHLHC12
• bHLHc12Max-interacting transcriptional repressor MAD4
• Class C basic helix-loop-helix protein 12
• Mad4 homolog
• MAD4Max-associated protein 4
• MAX dimerization protein 4
• Max dimerizer 4
• MST149
• MSTP149
• MXD4 MAX dimerization protein 4

Background

This gene is a member of the MAD gene family . The MAD genes encode basic helix loop helix leucine zipper proteins that heterodimerize with MAX protein, forming a transcriptional repression complex. The MAD proteins compete for MAX binding with MYC, which heterodimerizes with MAX forming a transcriptional activation complex. Studies in rodents suggest that the MAD genes are tumor suppressors and contribute to the regulation of cell growth in differentiating tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MAD4 Antibody (NB100-1117)(1)

Publication using NB100-1117

• Hurlin PJ, Queva C, Koskinen PJ et al. Mad3 and Mad4: novel Max-interacting transcriptional repressors that suppress c-myc dependent transformation and are expressed during neural and epidermal differentiation. EMBO J 1995-11-15 [PMID: 8521822]

Bioinformatics

• Gene Symbol: MXD4
• Entrez:
  • 10608(Human)
• Uniprot:
  • NP_006445.(Human)