Monkey M-CSF ELISA Kit (Colorimetric) Summary
| Description |
Assay Length: 1-5 hours |
| Standard Curve Range |
39.07 pg/mL - 2500 pg/mL |
| Sensitivity |
9.77 pg/mL |
| Assay Type |
Sandwich ELISA |
| Inter-Assay |
%CV < 10 |
| Intra-Assay |
%CV < 8 |
| Sample Volume |
50 - 100 uL |
| Kit Type |
ELISA Kit (Colorimetric) |
| Gene |
CSF1 |
Applications/Dilutions
Packaging, Storage & Formulations
| Storage |
Storage of components varies. See protocol for specific instructions. |
Kit Components
|
Components
|
- Adhesive Strip (For 96 wells)
- Assay plate (12 x 8 coated Microwells)
- Biotin-antibody (100x concentrate)
- Biotin-antibody Diluent
- HRP-avidin (100x concentrate)
- HRP-avidin Diluent
- Instruction manual
- Sample Diluent
- Standard (Freeze dried)
- Stop Solution
- TMB Substrate
- Wash Buffer (25x concentrate)
|
Alternate Names for Monkey M-CSF ELISA Kit (Colorimetric)
Background
M-CSF (macrophage colony stimulating factor, colony stimulating factor 1, CSF-1) is a hemtopoietic growth factor produced by monocytes, granulocytes, endothelial cells, fibroblasts and activated B-cells and T-cells. M-CSF stimulates macrophage colony formation and regulates proliferation and differentiation of hematopoeitic stem cells into macrophages and regulates survival and differentiation of monocytes. It enhances antibody-dependent cell-mediated cytotoxity, enhances marophage killing of tumor cells and microorganisms, regulates the release of cytokines and inhibits bone resorption by osteoclasts. Human M-CSF shows biological activity on murine and rat cells and although murine M-CSF is active in rat cells it is inactive in human cells.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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Product General Protocols
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FAQs for M-CSF ELISA Kit (NBP3-27874). (Showing 1 - 1 of 1 FAQ).
-
What is the difference between the Quantikine and Duoset ELISA kits?
- Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.