M-CSF Antibody (131614)

Recombinant Mouse M-CSF (Catalog # 416-ML) stimulates proliferation in the M-NFS-60 mouse myelogenous luekemia lymphoblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse M-CSF (10 ng/mL) is neutralized (green line) by increasing concentrations of Mouse M-CSF Monoclonal Antibody (Catalog # MAB4161). The ND50 is typically 3-12 µg/mL.

Injury-induced SSEA-1 positive cells were regulated by topical application of M-CSF or M-CSF neutralizing antibody.Skin wounds in mice were treated by either PBS control (top panels) or topical application of recombinant M-CSF protein (middle panels) or topical application of M-CSF neutralizing antibody for 6 days. This figure showed a representative skin section from 6 wounds in each group, stained with SSEA-1 (red color). DAPI (blue) was used for cell nucleus staining. Scale bars, 100 μm. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep28979), licensed under a CC-BY license. Not internally tested by R&D Systems.

SSEA-1 positive cells are also SSEA-3 positive and express M-CSF receptor.(A) Injured skin sections were stained with SSEA-1 (red color) and SSEA-3 (green color) antibodies. IgG was used for a negative staining control. DAPI (blue) was used for cell nucleus staining. Scale bars, 50 μm. (B) Injured skin sections were stained with SSEA-1 (green color) and M-CSF receptor (red color) antibodies. DAPI (blue) was used for cell nucleus staining. Scale bars, 50 μm. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep28979), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Mu
• Applications: WB, B/N
• Clone: 131614
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated

M-CSF Antibody (131614) Summary

• Immunogen: E. coli-derived recombinant mouse M-CSF
  Lys33-Glu262 (predicted)
  Accession # P07141
• Specificity: Detects mouse M-CSF in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human M-CSF or recombinant mouse SCF is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Gene: CSF1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Neutralization 3-12 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for M-CSF Antibody (131614)

• colony stimulating factor 1 (macrophage)
• CSF1
• CSF-1
• Lanimostim
• macrophage colony stimulating factor
• macrophage colony-stimulating factor 1
• MCSF
• M-CSF
• MCSFlanimostim
• MGC31930

Background

M-CSF, also known as CSF-1, is a four-alpha -helical-bundle cytokine that is the primary regulator of macrophage survival, proliferation and differentiation (1-3). M-CSF is also essential for the survival and proliferation of osteoclast progenitors (1, 4). M-CSF also primes and enhances macrophage killing of tumor cells and microorganisms, regulates the release of cytokines and other inflammatory modulators from macrophages, and stimulates pinocytosis (2, 3). M-CSF increases during pregnancy to support implantation and growth of the decidua and placenta (5). Sources of M-CSF include fibroblasts, activated macrophages, endometrial secretory epithelium, bone marrow stromal cells, and activated endothelial cells (1-5). The M-CSF receptor (c-fms) transduces its pleotropic effects and mediates its endocytosis. M-CSF mRNAs of various sizes occur (3-9). Full length mouse M-CSF transcripts encode a 520 amino acid (aa) type I transmembrane (TM) protein with a 462 aa extracellular region, a 21 aa TM domain, and a 37 aa cytoplasmic tail that forms a 140 kDa covalent dimer. Differential processing produces two proteolytically cleaved, secreted dimers. One is an N- and O-glycosylated 86 kDa dimer, while the other is modified by both glycosylation and chondroitin-sulfate proteoglycan (PG) to generate a 200 kDa subunit. Although PG-modified M-CSF can circulate, it may be immobilized by attachment to type V collagen (8). Shorter transcripts encode M-CSF that lacks cleavage and PG sites and produces an N-glycosylated 68 kDa TM dimer and a slowly produced 44 kDa secreted dimer (7). Although forms may vary in activity and half-life, all contain the N-terminal 150 aa portion that is necessary and sufficient for interaction with the M-CSF receptor (10, 11). The first 229 aa of mature mouse M-CSF shares 87%, 83%, 82%, and 81% aa identity with corresponding regions of rat, dog, cow, and human M-CSF, respectively (12, 13). Human M-CSF is active in the mouse, but mouse M-CSF is reported to be species-specific.

1. Pixley, F.J. and E.R. Stanley (2004) Trends Cell Biol. 14:628.
2. Chitu, V. and E.R. Stanley (2006) Curr. Opin. Immunol. 18:39.
3. Fixe, P. and V. Praloran (1997) Eur. Cytokine Netw. 8:125.
4. Ryan, G.R. et al. (2001) Blood 98:74.
5. Makrigiannakis, A. et al. (2006) Trends Endocrinol. Metab. 17:178.
6. Nandi, S. et al. (2006) Blood 107:786.
7. Rettenmier, C.W. and M.F. Roussel (1988) Mol. Cell Biol. 8:5026.
8. Suzu, S. et al. (1992) J. Biol. Chem. 267:16812.
9. Manos, M.M. (1988) Mol. Cell. Biol. 8:5035.
10. Koths, K. (1997) Mol. Reprod. Dev. 46:31.
11. Jang, M-H. et al. (2006) J. Immunol. 177:4055.
12. DeLamarter, J.F. et al. (1987) Nucleic Acids Res. 15:2389.
13. Ladner, M.B. et al. (1988) Proc. Natl. Acad. Sci. USA 85:6706.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for M-CSF Antibody (MAB4161)(7)

Publications using MAB4161

• V Grubiši?, JL McClain, DE Fried, I Grants, P Rajasekhar, E Csizmadia, OA Ajijola, RE Watson, DP Poole, SC Robson, FL Christofi, BD Gulbransen Enteric Glia Modulate Macrophage Phenotype and Visceral Sensitivity following Inflammation Cell Rep, 2020-09-08;32(10):108100. 2020-09-08 [PMID: 32905782] (Activation, Mouse)
• D Lu, Y Liao, SH Zhu, QC Chen, DM Xie, JJ Liao, X Feng, MH Jiang, W He Bone-derived Nestin-positive mesenchymal stem cells improve cardiac function via recruiting cardiac endothelial cells after myocardial infarction Stem Cell Res Ther, 2019-04-27;10(1):127. 2019-04-27 [PMID: 31029167] (Western Blot, N/A)
• T Kobori, S Hamasaki, A Kitaura, Y Yamazaki, T Nishinaka, A Niwa, S Nakao, H Wake, S Mori, T Yoshino, M Nishibori, H Takahashi Interleukin-18 Amplifies Macrophage Polarization and Morphological Alteration, Leading to Excessive Angiogenesis Front Immunol, 2018-03-06;9(0):334. 2018-03-06 [PMID: 29559970] (Neutralization, Western Blot, Mouse)
• K Choi, K Komurov, JS Fletcher, E Jousma, JA Cancelas, J Wu, N Ratner An inflammatory gene signature distinguishes neurofibroma Schwann cells and macrophages from cells in the normal peripheral nervous system Sci Rep, 2017-03-03;7(0):43315. 2017-03-03 [PMID: 28256556] (Neutralization, Mouse)
• Yamane F, Nishikawa Y, Matsui K, Asakura M, Iwasaki E, Watanabe K, Tanimoto H, Sano H, Fujiwara Y, Stanley E, Kanayama N, Mabbott N, Magari M, Ohmori H CSF-1 receptor-mediated differentiation of a new type of monocytic cell with B cell-stimulating activity: its selective dependence on IL-34. J Leukoc Biol, 2013-09-19;95(1):19-31. 2013-09-19 [PMID: 24052571] (Western Blot, Mouse)
• Luo , Jian, Elwood , Fiona, Britschgi , Markus, Villeda , Saul, Zhang , Hui, Ding , Zhaoqing, Zhu , Liyin, Alabsi , Haitham, Getachew , Ruth, Narasimhan , Ramya, Wabl , Rafael, Fainberg , Nina, James , Michelle, Wong , Gordon, Relton , Jane, Gambhir , Sanjiv S, Pollard , Jeffrey, Wyss-Coray , Tony Colony-stimulating factor 1 receptor (CSF1R) signaling in injured neurons facilitates protection and survival. J Exp Med, 2013-01-07;210(1):157-72. 2013-01-07 [PMID: 23296467] (IHC-Fr, Mouse)
• Zhu J, Garrett R, Jung Y, Zhang Y, Kim N, Wang J, Joe GJ, Hexner E, Choi Y, Taichman RS, Emerson SG Osteoblasts support B-lymphocyte commitment and differentiation from hematopoietic stem cells. Blood, 2007-01-16;109(9):3706-12. 2007-01-16 [PMID: 17227831] (Neutralization, Mouse)

Bioinformatics

• Gene Symbol: CSF1
• Uniprot:
  • P07141()