LYVE-1 Antibody [DyLight 488]

Images

 
Immunohistochemistry-Paraffin: LYVE1 Antibody [DyLight 488] [NB100-725G] - IHC analysis of LYVE1 in paraffin embedded MDA-MB-231 breast cancer orthotopic transplantation tissue. Image courtesy of product review ...read more
Flow Cytometry: LYVE1 Antibody [DyLight 488] [NB100-725G] - Unlabeled control with labeled samples of cells dissociated mechanically and enzymatically from human skin. Image provided via product review by Patricia ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
DyLight 488

LYVE-1 Antibody [DyLight 488] Summary

Immunogen
A synthetic peptide made to a C-terminal portion of the mouse LYVE1 protein sequence (between residues 250-318). [UniProt# Q8BHC0]
Localization
Plasma membrane
Marker
Lymphatic Vessel Marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
LYVE1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
Application Notes
This LYVE1 antibody is useful for Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin and Western Blot where a band at ~45 kDa is seen. For Immunohistochemistry citrate buffer antigen retrieval is recommended.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 2 Reviews rated 5
using
NB100-725G in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for LYVE-1 Antibody [DyLight 488]

  • cell surface retention sequence binding protein-1
  • Cell surface retention sequence-binding protein 1
  • CRSBP1
  • CRSBP-1
  • extracellular link domain containing 1
  • extracellular link domain-containing 1
  • Extracellular link domain-containing protein 1
  • HAR
  • Hyaluronic acid receptor
  • lymphatic vessel endothelial hyaluronan receptor 1
  • lymphatic vessel endothelial hyaluronic acid receptor 1
  • LYVE1
  • LYVE-1
  • LYVE-1XLKD1
  • XLKD1

Background

The lymphatic vasculature forms a second circulatory system that drains extracellular fluid from the tissues and provides an exclusive environment in which immune cells can encounter and respond to foreign antigen. Recently a number of interesting molecules have been identified that may be exploited as markers for lymphatic endothelium, including the hyaluronan receptor LYVE1, PALE, VEGFR3, and podoplanin. LYVE1 has been identified as a major receptor for HA (extracellular matrix glycosaminoglycan hyaluronan) on the lymph vessel wall. The deduced amino acid sequence of LYVE1 predicts a 322-residue type I integral membrane polypeptide 41% similar to the CD44 HA receptor with a 212-residue extracellular domain containing a single Link module, the prototypic HA binding domain of the Link protein superfamily. Like CD44, the LYVE1 molecule binds both soluble and immobilized HA. However, unlike CD44, the LYVE1 molecule colocalizes with HA on the luminal face of the lymph vessel wall and is completely absent from blood vessels. Hence, LYVE1 is the first lymph-specific HA receptor to be characterized and is a uniquely powerful marker for lymph vessels themselves.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Reviews for LYVE-1 Antibody (NB100-725G) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.
We have 2 reviews tested in 2 application: Flow, IHC-P.

Reviews using NB100-725G:
Filter by Applications
Flow
(1)
IHC-P
(1)
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Filter by Species
Human
(2)
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Images Ratings Applications Species Date Details
Flow Cytometry LYVE-1 NB100-725G
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reviewed by:
Anonymous
Flow Human 06/18/2013
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Summary

ApplicationFlow Cytometry
Sample TestedStromal vascular fraction from skin
SpeciesHuman
CommentsProduct Review courtesy of Patricia Redondo.

Blocking

Blocking DetailsFc Block from Myltenyi 20 minutes on ice

Primary Anitbody

Dilution Ratio1uL of antibody to 80uL of sample containing 1.000.000 cells 30 minutes on ice

Details

Detection NotesFACSCallibur and Cyan
Permeabilization DetailsSample pellet was immediately labelled with the antibody after Fc blocking
Wash Descriptionno washing after Fc blocking, 2x washing after antibody staining with PBS

Comments

CommentsProduct Review courtesy of Patricia Redondo.
Immunohistochemistry-Paraffin LYVE-1 NB100-725G
Enlarge
reviewed by:
Anonymous
IHC-P Human 11/16/2010
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedMDA-MB-231 breast cancer orthotopic transplantation
SpeciesHuman
LotA-A
CommentsSpecific staining with no background. Detection Method: DAB, Positive Control: mouse lymphnodes and human tonsils, Negative Control: human breast cancer tissue, Deparaffinization:.xylene and graded series of alcohols, Antigen Retrieval: citrate buffer pH6, 30 minutes at 100 degrees Celsius

Blocking

Blocking DetailsBlocking Buffer: Protein Block Serum-Free (from Dako), Blocking Time: 10 minutes, Blocking Temp: RT

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:100, Primary Ab Incubation Time: 1 hour, Primary Ab Incubation Temp: room temperature

Secondary Antibody

Secondary DescriptionSecondary Ab: Labeled Streptavidin Biotin+System-HRP kit
Secondary Manufacturer Cat#DAKO, cat#: K0679

Details

Detection NotesPlease see Comments
Fixation DetailsSample Preparation: buffered 10% formalin and paraffin embedded, Deparaffinization:.xylene and graded series of alcohols

Comments

CommentsSpecific staining with no background. Detection Method: DAB, Positive Control: mouse lymphnodes and human tonsils, Negative Control: human breast cancer tissue, Deparaffinization:.xylene and graded series of alcohols, Antigen Retrieval: citrate buffer pH6, 30 minutes at 100 degrees Celsius

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for LYVE-1 Antibody (NB100-725G) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

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Recent Reviews

5
5
2
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0
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0
2
0
1
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Anonymous
06/18/2013
Application: Flow
Species: Human

Anonymous
11/16/2010
Application: IHC-P
Species: Human

Bioinformatics

Gene Symbol LYVE1
Entrez