LYVE-1 Antibody [Biotin]

2089-LY) was serially diluted and captured by Mouse Anti-Human LYVE‑1 Monoclonal Antibody (Catalog # MAB20891) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human LYVE‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2089) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" > 2089-LY) was serially diluted and captured by Mouse Anti-Human LYVE‑1 Monoclonal Antibody (Catalog # MAB20891) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human LYVE‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2089) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" title="Recombinant Human LYVE‑1 (Catalog # 2089-LY) was serially diluted and captured by Mouse Anti-Human LYVE‑1 Monoclonal Antibody (Catalog # MAB20891) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human LYVE‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2089) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" />

Recombinant Human LYVE‑1 (Catalog # <a class=

• Applications: WB, IHC, ELISA(Det)
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

LYVE-1 Antibody [Biotin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human LYVE-1
  Ser24-Thr238
  Accession # Q9Y5Y7
• Specificity: Detects LYVE‑1 in ELISAs and Western blots. In sandwich immunoassays, less than 0.1% cross-reactivity with recombinant mouse LYVE-1 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: LYVE1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL
• Application Notes: ELISA Capture: Human LYVE-1 Antibody (Catalog # MAB20891)
  ELISA Detection: Human LYVE-1 Biotinylated Antibody (Catalog # BAF2089)
  Standard: Recombinant Human LYVE-1 (Catalog # 2089-LY)
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for LYVE-1 Antibody [Biotin]

• cell surface retention sequence binding protein-1
• Cell surface retention sequence-binding protein 1
• CRSBP1
• CRSBP-1
• extracellular link domain containing 1
• extracellular link domain-containing 1
• Extracellular link domain-containing protein 1
• HAR
• Hyaluronic acid receptor
• lymphatic vessel endothelial hyaluronan receptor 1
• lymphatic vessel endothelial hyaluronic acid receptor 1
• LYVE1
• LYVE-1
• UNQ230/PRO263
• XLKD1

Background

Lymphatic vessel endothelial hyaluronan (HA) receptor-1 (LYVE-1) is a receptor of HA, a linear high molecular weight polymer composed of alternating units of D‑glucuronic acid and N-acetyl-D-glucosamine. HA is found in the extracellular matrix of most animal tissues and in body fluids. It modulates cell behavior and functions during tissue remodeling, development, homeostasis, and disease (1). The turnover of HA (several grams/day in humans) occurs primarily in the lymphatics and liver, the two major clearance systems that catabolize approximately 85% and 15% of HA, respectively (1-3). LYVE-1 shares 41% homology with the other known HA receptor, CD44 (4). The homology between the two proteins increases to 61% within the HA binding domain. The HA binding domain, known as the link module, is a common structural motif found in other HA binding proteins such as link protein, aggrecan and versican (1, 5). Human and mouse LYVE-1 share 69% amino acid sequence identity.

LYVE-1 is primarily expressed on both the luminal and abluminal surfaces of lymphatic vessels (4, 5). In addition, LYVE-1 is also present in normal hepatic blood sinusoidal endothelial cells (6). LYVE-1 mediates the endocytosis of HA and may transport HA from tissue to lymph by transcytosis, delivering HA to lymphatic capillaries for removal and degradation in the regional lymph nodes (5, 7, 8). Because of its restricted expression patterns, LYVE-1, along with other lymphatic proteins such as VEGF R3, podoplanin and the homeobox protein propero-related (Prox-1), constitute a set of markers useful for distinguishing between lymphatic and blood microvasculature (4, 5, 9-11).

1. Knudson, C.B. and W. Knudson (1993) FASEB J. 7:1233.
2. Evered, D and J. Whelan (1989) Ciba Found. Symp. 143:1.
3. Laurent, T.C. and J.R.F. Fraser (1992) FASEB J. 6:2397.
4. Banerji, S. et al. (1999) J. Cell Biol. 144:789.
5. Prevo, R. et al. (2001) J. Biol. Chem. 276:19420.
6. Jackson, D.J. et al. (2001)Trends Immunol. 22:317.
7. Zhou, B. et al. (2000) J. Biol. Chem. 275:37733.
8. Achen, M. et al. (1998) Proc. Natl. Acad. Sci. USA 95:548.
9. Breiteneder-Gellef, S. et al. (1999) Am. J. Pathol. 154:385.
10. Wiggle, J.T. and G. Oliver (1999) Cell 98:769.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for LYVE-1 Antibody (BAF2089)(2)

Publications using BAF2089

• TA Stanly, M Fritzsche, S Banerji, D Shrestha, F Schneider, C Eggeling, DG Jackson The cortical actin network regulates avidity-dependent binding of hyaluronan by the Lymphatic Vessel Endothelial receptor LYVE-1 J. Biol. Chem., 2020-02-07;0(0):. 2020-02-07 [PMID: 32034091] (Immunoprecipitation, Human)
• Huber S, Bruns CJ, Schmid G, Hermann PC, Conrad C, Niess H, Huss R, Graeb C, Jauch KW, Heeschen C, Guba M Inhibition of the mammalian target of rapamycin impedes lymphangiogenesis. Kidney Int., 2007-02-14;71(8):771-7. 2007-02-14 [PMID: 17299523] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: LYVE1
• Uniprot:
  • Q9Y5Y7()