Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody

Western blot shows lysates of 293T human embryonic kidney cell line and MOLT-4 human acute lymphoblastic leukemia cell line. Gels were loaded with 30 µg of whole cell lysate (WCL), 20 µg of cytoplasmic (Cyto), and 10 µg of nuclear extracts (Nuc). PVDF Membrane was probed with 2 µg/mL of Sheep Anti-Human KDM3A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6746) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for KDM3A at approximately 150 kDa (as indicated), the band at approximately 70kDa represents the product of degradation or processing. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody Summary

• Immunogen: E. coli-derived recombinant human KDM3A
  Pro844-Glu968
  Accession # Q9Y4C1
• Specificity: Detects human KDM3A in direct ELISAs and Western blots. In direct ELISAs, approximately 3% cross-reactivity with recombinant human (rh) KDM4A is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: KDM3A
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody

• EC 1.14.11
• EC 1.14.11.-
• JHDM2A
• JHDM2AJMJD1AJHMD2A
• JmjC domain-containing histone demethylation protein 2A
• JMJD1
• JMJD1A
• jumonji C domain-containing histone demethylase 2A
• jumonji domain containing 1
• jumonji domain containing 1A
• Jumonji domain-containing protein 1A
• KDM3A
• KIAA0742
• KIAA0742DKFZp686A24246
• Lysine (K)specific Demethylase 3A
• Lysine (K)-specific Demethylase 3A
• lysine-specific demethylase 3A
• testis-specific protein A
• TSGA
• TSGADKFZp686P07111

Background

JMJD1A (Jumonji [Japanese for "cruciform/cross"]/ARID domain-containing protein 1A; also KDM3A, JHDM2A, and TSGA) is a 140-150 kDa member of the JHDM2 histone demethylase family of proteins. It is induced by hypoxia, and expressed in spermatids, smooth muscle cells, mammary epithelium, hepatocytes, and renal tubular epithelium. JMJD1A is reported to demethylate di- and mono-methylated Lys9 on histone H3. It has no activity on trimethylated Lys9. JMJD1A is both cytoplasmuc and nuclear, and serves as a coactivator for the AR. Human JMJD1A is 1321 amino acids (aa) in length. It contains one C6-type zinc-finger motif (aa 662-687) and a jumonji C type catalytic domain (aa 1151-1264). There is one phosphorylation site at Ser445. There is one potential splice variants that shows a 13 aa substitution for aa 699-1321. Over aa 810-968, human JMJD1A shares 94% aa identity with mouse JMJD1A.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KDM3A
• Uniprot:
  • Q9Y4C1()