Lymphotoxin beta R/TNFRSF3 Antibody (4H8 WH2)

Product Discontinued

Lymphotoxin beta R/TNFRSF3 Antibody (4H8 WH2) Summary

• Immunogen: Recombinant mouse Lymphotoxin-beta Receptor cysteine-rich region (aa 31-221).
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: LTBR
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10-1:1000
  • Functional
• Application Notes: This antibody is useful in Flow Cytometry, Functional Application (agonist inducing BAFF, chemokines and integrins in vitro and in vivo).

Reactivity Notes

Mouse

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Liquid. In PBS containing 10% glycerol.
• Preservative: No Preservative
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Notes

The monoclonal antibody to mouse LTBR is an agonist that can be used for the investigation of the regulation of BAFF(BlyS), chemokines and integrins using in vivo and tissue culture models, the development of NK cells, and NK T cells, to study the regulation of NF-?B family of transcription factors in regulation of inflammation and homeostasis, particularly RelB NF-?B2 pathway. For use as an agonist the MAb to LTBR is added to cell cultures at 2ug/ml. For in vivo use, mice are injected intraperitoneally with 50ug of agonistic MAb to LTBR in sterile phosphate saline.

Alternate Names for Lymphotoxin beta R/TNFRSF3 Antibody (4H8 WH2)

• CD18
• D12S370
• ltbetar
• LT-BETA-R
• LTBR
• lymphotoxin B receptor
• Lymphotoxin beta R
• lymphotoxin beta receptor (TNFR superfamily, member 3)
• Lymphotoxin-beta receptor
• LymphotoxinbR
• TNF RIII
• TNF Rrp
• TNFCRTNF-RIII
• TNFR superfamily, member 3
• TNFR2-RP
• TNFR3
• TNF-R-III
• TNFR-RP
• TNFRSF3
• TNFRSF3TNFR-III
• Tumor necrosis factor C receptor
• Tumor necrosis factor receptor 2-related protein
• tumor necrosis factor receptor superfamily member 3
• tumor necrosis factor receptor superfamily, member 3
• Tumor necrosis factor receptor type III

Background

The LTBR activates two different NF-?B pathways that lead to distinct patterns of gene induction, including selected chemokines, and the cytokine BAFF, which is essential for the survival of mature B lymphocytes. LTBR activates the classical NF-?B (relA/p50) pathway, like the type 1 TNF receptor (TNFR1), that regulates proinflammatory genes, like the chemokine MIP1B. However, LTBR, unlike TNFR1, also activates the processing of p100 to form RelB/p52 complexes, which activate genes involved in lymphoid organ formation and lymphocyte survival.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Lymphotoxin beta R/TNFRSF3 Antibody (NBP1-97658)(3)

Publications using NBP1-97658

• Enwere EK, Holbrook J, Lejmi-Mrad R et al. TWEAK and cIAP1 Regulate Myoblast Fusion Through the Noncanonical NF-?B Signaling Pathway Sci Signal 2012-10-16 [PMID: 23074266]
• Silva-Santos B, Pennington DJ, Hayday AC et al. Lymphotoxin-mediated regulation of gammadelta cell differentiation by alphabeta T cell progenitors. Science. 2005-02-01 [PMID: 15591166]
• Dejardin E, Droin NM, Delhase M et al. The lymphotoxin-beta receptor induces different patterns of gene expression via two NF-kappaB pathways. Immunity. 2002-10-01 [PMID: 12387745]

Bioinformatics

• Gene Symbol: LTBR