Lymphotoxin beta R/TNFRSF3 Antibody (157108) [Unconjugated]

NIH-3T3 mouse embryonic fibroblast cell line was stained with Rat Anti-Mouse Lymphotoxin beta R/TNFRSF3 Monoclonal Antibody (Catalog # MAB10081, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Mu
• Applications: WB, Flow, CyTOF-ready
• Clone: 157108
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

Lymphotoxin beta R/TNFRSF3 Antibody (157108) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Lymphotoxin beta R/TNFRSF3
  Ser28-Pro218
  Accession # P50284
• Specificity: Detects mouse Lymphotoxin beta R in direct ELISAs and Western blots. In direct ELISAs, does not cross-react with recombinant mouse (rm) 4‑1BB, rmBAFF R, rmCD27, rmCD30, rmCD40, recombinant human (rh) DR3, rhDR6, rmEDAR, rmFas, rmGITR, rmHVEM, rhLymphotoxin beta R, rmNGF R, rmOPG, rmOX40, rmRANK, rhRELT, rmTAJ, rmTNF RI, rmTNF RII, rhTRADD, rmTRAIL R2, rhTRAIL R3, rhTRAIL R4, or rmTWEAK R.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: LTBR
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Lymphotoxin beta R/TNFRSF3 Antibody (157108) [Unconjugated]

• CD18
• D12S370
• ltbetar
• LT-BETA-R
• LTBR
• lymphotoxin B receptor
• Lymphotoxin beta R
• lymphotoxin beta receptor (TNFR superfamily, member 3)
• Lymphotoxin-beta receptor
• LymphotoxinbR
• TNF RIII
• TNF Rrp
• TNFCRTNF-RIII
• TNFR superfamily, member 3
• TNFR2-RP
• TNFR3
• TNF-R-III
• TNFR-RP
• TNFRSF3
• TNFRSF3TNFR-III
• Tumor necrosis factor C receptor
• Tumor necrosis factor receptor 2-related protein
• tumor necrosis factor receptor superfamily member 3
• tumor necrosis factor receptor superfamily, member 3
• Tumor necrosis factor receptor type III

Background

Lymphotoxin beta R, now designated TNFRSF3, is a cell surface protein expressed in a variety of tissues. Lymphotoxin beta R transduces signals following binding of LIGHT or the heterotrimeric lymphotoxin LT alpha 1/ beta 2 or LT alpha 2/ beta 1. It plays a critical role in controlling cellular immune functions and lymphoid organogenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Lymphotoxin beta R/TNFRSF3 Antibody (MAB10081)(1)

Publication using MAB10081

• Brightbill H, Jackman J, Suto E, Kennedy H, Jones C, Chalasani S, Lin Z, Tam L, Roose-Girma M, Balazs M, Austin C, Lee W, Wu L Conditional Deletion of NF-kappaB-Inducing Kinase (NIK) in Adult Mice Disrupts Mature B Cell Survival and Activation. J Immunol, 2015-06-26;195(3):953-64. 2015-06-26 [PMID: 26116508] (Bioassay, Mouse)

Bioinformatics

• Gene Symbol: LTBR
• Uniprot:
  • P50284()