Ly-6G/Ly-6C Antibody (RB6-8C5)

Images

 
Immunohistochemistry-Paraffin: Ly-6G/Ly-6C (Gr-1) Antibody [NBP2-00441] - IHC analysis of a formalin fixed paraffin embedded tissue section of mouse bone marrow using 1:200 dilution of Lot A-1 of Ly-6G antibody (clone ...read more
Flow Cytometry: Ly-6G/Ly-6C (Gr-1) Antibody [NBP2-00441] - Analysis using the Allophycocyanin conjugate of NBP2-00441. Staining of C57BL/6 bone marrow cells with 0.125 ug of Rat IgG2b kappa Isotype Control APC (blue ...read more
Immunohistochemistry-Paraffin: Ly-6G/Ly-6C (Gr-1) Antibody [NBP2-00441] - IHC analysis of a formalin fixed paraffin embedded tissue section of mouse bone marrow using 1:100 dilution of Lot E04586-1632 of Ly-6G antibody ...read more
Flow Cytometry: Ly-6G/Ly-6C (Gr-1) Antibody [NBP2-00441] - Staining of mouse bone marrow with Anti-Mouse Ly-6G (Gr-1) FITC and Anti-Human/Mouse CD45R (B220) PE. Total viable cells were used for analysis.
Flow Cytometry: Ly-6G/Ly-6C (Gr-1) Antibody [NBP2-00441] - 1 ug/1^6 BALB/c bone marrow cells were stained with either rat IgG2b: FITC (as an isotype control) or rat anti-mouse Ly-6G: FITC. Large cells were then gated ...read more
Flow Cytometry: Ly-6G/Ly-6C (Gr-1) Antibody [NBP2-00441] - Analysis using the PE conjugate of NBP2-00441. Total BALB/c bone marrow cell suspension was stained with Anti-Mouse Ly-6G (Gr-1) PE. Viable cells were gated on ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, Flow, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready
Clone
RB6-8C5
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Ly-6G/Ly-6C Antibody (RB6-8C5) Summary

Immunogen
Normal mouse bone marrow.
Specificity
Studies indicate that in the bone marrow and lysed whole blood, the antibody clone RB6-8C5 also reacts with an additional subpopulation of Ly-6C high cells.
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Rat
Gene
LY6G
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Flow Cytometry 1:10-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • CyTOF-ready
Application Notes
The RB6-8C5 antibody has been tested by flow cytometric analysis of mouse bone marrow cells and splenocyte suspensions. This can be used at less than or equal to 0.5 ug per test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 uL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. The RB6-8C5 antibody has also been reported for use immunoprecipitation, immunoblotting (WB) and immunohistochemical staining. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. In vivo assay was reported in scientific literature.
Reviewed Applications
Read 1 Review rated 4
using
NBP2-00441 in the following applications:

Publications
Read Publications using
NBP2-00441 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A or G purified

Background

The RB6-8C5 monoclonal antibody reacts with mouse Ly-6G, a 21-25 kDa protein also known as the myeloid differentiation antigen Gr-1. A GPIlinked protein, Gr-1 is expressed by the myeloid lineage in a developmentally regulated manner in the bone marrow. While monocytes only express Gr-1 transiently during their bone marrow development, the expression of Gr-1 on bone marrow granulocytes as well as on peripheral neutrophils is a good marker for these populations.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Ly-6G/Ly-6C Antibody (NBP2-00441)(10)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 5 applications: FLOW, IHC, IHC-Fr, IHC-P, In vivo.


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FLOW
(1)
IHC
(1)
IHC-Fr
(2)
IHC-P
(1)
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(2)
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Showing Publications 1 - 10 of 10.
Publications using NBP2-00441 Applications Species
Pickens SR, Chamberlain ND, Volin MV et al. Local expression of interleukin-27 ameliorates collagen-induced arthritis Arthritis Rheum 2011 Aug [PMID: 21384333] (IHC, Mouse) IHC Mouse
Huang B, Zhao J, Shen S, Li H, He KL, Shen GX, Mayer L, Unkeless J, Li D, Yuan Y, Zhang GM, Xiong H, Feng ZH. Listeria monocytogenes promotes tumor growth via tumor cell toll-like receptor 2 signaling. Cancer Res;67(9):4346-52. 2007 May 1. [PMID: 17483348] (In vivo) In vivo
Sasmono RT, Ehrnsperger A, Cronau SL, Ravasi T, Kandane R, Hickey MJ, Cook AD, Himes SR, Hamilton JA, Hume DA. Mouse neutrophilic granulocytes express mRNA encoding the macrophage colony-stimulating factor receptor (CSF-1R) as well as many other macrophage-specific transcripts and can transdifferentiate into macrophages in vitro in response to CSF-1. J Leukoc Biol;82(1):111-23. 2007 Jul. [PMID: 17438263]
Fan J, Li Y, Vodovotz Y, Billiar TR, Wilson MA. Hemorrhagic shock-activated neutrophils augment TLR4 signaling-induced TLR2 upregulation in alveolar macrophages: role in hemorrhage-primed lung inflammation. Am J Physiol Lung Cell Mol Physiol;290(4):L738-L746. 2006 Apr. [PMID: 16272176] (In vivo) In vivo
Alvarez MJ, Prada F, Salvatierra E, Bravo AI, Lutzky VP, Carbone C, Pitossi FJ, Chuluyan HE, Podhajcer OL. Secreted protein acidic and rich in cysteine produced by human melanoma cells modulates polymorphonuclear leukocyte recruitment and antitumor cytotoxic capacity. Cancer Res;65(12):5123-32. 2005 Jun 15. [PMID: 15958556]
Brown CR, Blaho VA, Loiacono CM. Susceptibility to experimental Lyme arthritis correlates with KC and monocyte chemoattractant protein-1 production in joints and requires neutrophil recruitment via CXCR2. J Immunol;171(2):893-901. 2003 Jul 15. [PMID: 12847259] (IHC-P) IHC-P
McLellan AD, Kapp M, Eggert A et al. Anatomic location and T-cell stimulatory functions of mouse dendritic cell subsets defined by CD4 and CD8 expression. Blood. [PMID: 11877283] (FLOW, Mouse) FLOW Mouse
Miura M, Fu X, Zhang QW, Remick DG, Fairchild RL. Neutralization of Gro alpha and macrophage inflammatory protein-2 attenuates renal ischemia/reperfusion injury. Am J Pathol;159(6):2137-45. 2001 Dec. [PMID: 11733364] (IHC-Fr) IHC-Fr
Dunussi-Joannopoulos K, Runyon K, Erickson J, Schaub RG, Hawley RG, Leonard JP. Vaccines with interleukin-12-transduced acute myeloid leukemia cells elicit very potent therapeutic and long-lasting protective immunity. Blood;94(12):4263-73. 1999 Dec 15. [PMID: 10590071] (IHC-Fr) IHC-Fr
Fleming TJ, Fleming ML, Malek TR. Selective expression of Ly-6G on myeloid lineage cells in mouse bone marrow.RB6-8C5 mAb to granulocytedifferentiation antigen (Gr-1) detects members of the Ly-6 family. J Immunol;151(5):2399-408. 1993 Sep 1. [PMID: 8360469]

Review for Ly-6G/Ly-6C Antibody (NBP2-00441) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.
We have 1 review tested in 1 application: IHC-P.

Reviews using NBP2-00441:
Filter by Applications
IHC-P
(1)
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Filter by Species
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin Ly-6G/Ly-6C NBP2-00441
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reviewed by:
Anonymous
IHC-P Mouse 02/24/2017
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Summary

ApplicationImmunohistochemistry-Paraffin
Sample Testedmouse skin
SpeciesMouse

Comments

Commentsdilution 1:100
Overnight incubation

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Ly-6G/Ly-6C Antibody (NBP2-00441). (Showing 1 - 2 of 2 FAQs).

  1. On the Datasheet of Ly-6G Antibody (RB6-8C5), Western Blot is listed as one of the applications. Do you have any suggestions on the concentration to use for this Ab in WB?
    • We recommend dilutions between 1:1000 and 1:2000 as a starting point in Western blot.
  2. Can you please provide me with any publication reference the uses clone RB6-8C5 in Western?
    • Unfortunately we do not have any references for use with this antibody in Western blot at the time.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP2-00441AF405
Alexa Fluor 488 Labeled NBP2-00441AF488
Alexa Fluor 647 Labeled NBP2-00441AF647
Alexa Fluor 700 Labeled NBP2-00441AF700
Allophycocyanin Labeled NBP2-00372
Biotin Labeled NBP1-28168
DyLight 350 Labeled NBP2-00441UV
DyLight 405 Labeled NBP2-00441V
DyLight 488 Labeled NBP2-00441G
DyLight 550 Labeled NBP2-00441R
DyLight 650 Labeled NBP2-00441C
DyLight 680 Labeled NBP2-00441FR
DyLight 755 Labeled NBP2-00441IR
HRP Labeled NBP2-00441H
PE Labeled NBP2-00440
PerCP Labeled NBP2-00441PCP

Additional Ly-6G/Ly-6C Products

Ly-6G/Ly-6C NBP2-00441

Bioinformatics Tool for Ly-6G/Ly-6C Antibody (NBP2-00441)

Discover related pathways, diseases and genes to Ly-6G/Ly-6C Antibody (NBP2-00441). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Recent Reviews

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1
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Anonymous
02/24/2017
Application: IHC-P
Species: Mouse

Bioinformatics

Gene Symbol LY6G