LRRK2 Antibody - BSA Free

Western Blot: LRRK2 Antibody [NB110-55289] - Detection of murine LRRK2 in mouse brain lysate.

Immunohistochemistry: LRRK2 Antibody [NB110-55289] - Staining of neurons and glia. Mouse Brain, 40X.

• Reactivity: Mu, Hu(-)
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.09 mg/ml

LRRK2 Antibody - BSA Free Summary

• Immunogen: A synthetic peptide made to an internal region of the mouse LRRK2 protein sequence (between residues 900-1000). [Swiss-Prot: Q5S006]
• Localization: Mitochondrial outer membrane
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: LRRK2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 2.5 ug/ml
  • Immunohistochemistry-Paraffin 2.5 ug/ml
  • Western Blot 2 ug/ml
• Application Notes: In Western blot, a band is seen at ~284 kDa. There is a band at ~171 and 117 kDa, as well as several lower molecular weight, non-specific bands.
• Theoretical MW: 285 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Mouse Brain Whole Tissue Lysate (Adult Whole Normal)

• Control Peptide:

  LRRK2 Peptide (NB110-55289PEP)

Reactivity Notes

Does not react with Human.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.1% Sodium Azide
• Concentration: 1.09 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for LRRK2 Antibody - BSA Free

• augmented in rheumatoid arthritis 17
• AURA17
• Dardarin
• DKFZp434H2111
• EC 2.7.11.1
• FLJ45829
• leucine-rich repeat kinase 2
• leucine-rich repeat serine/threonine-protein kinase 2
• LRRK2
• PARK8
• PARK8DARDARIN
• Parkinson disease (autosomal dominant) 8
• RIPK7
• ROCO2

Background

LRRK2 (leucine-rich repeat serine/threonine-protein kinase 2, also called PARK8 or Dardarin) is a homodimeric multifunctional protein that belongs to TKL serine threonine protein kinases family and LRRK2 immunoreactivity has been documented in neurofibrillary tangles in Alzheimer's disease as well as in PDCG (parkinsonism-dementia complex of Guam). Highly expressed in tissues such as liver, heart, brain etc., LRRK2 localizes to cellular cytoplasm and also associates with membrane structures including mitochondrial outer membrane. LRRK interacts with PARK2, PRDX3 and TPCN2, and phosphorylate proteins which are implicated in Parkinson disease. LRRK2 is a cytoplasmic kinase with autophosphorylation capability as well as GTPase activity. Its interaction with microtubules suggests that LRRK2-induced neurodegeneration might be partly mediated via inhibition of microtubule dynamics, and LRRK2 -mitochondria interactiosn suggests its implication in pathways eliciting ROS mediated damage. LRRK2 has been shown exert positive regulation in autophagy via calcium-sensitive activation of CaMKK/AMPK signaling pathway through activation of NAADP receptors, increase of lysosomal pH as well as calcium release.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: LRRK2