LRP-6 Antibody

Immunohistochemistry-Paraffin: LRP-6 Antibody [NB100-1475] - Analysis of paraffin embedded Human Kidney using this antibody at 4 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

Flow Cytometry: LRP-6 Antibody [NB100-1475] - Flow cytometric analysis of paraformaldehyde fixed K562 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

• Reactivity: Hu, Mu, Rt, Ca
• Applications: WB, ELISA, Flow, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

LRP-6 Antibody Summary

• Immunogen: Peptide with sequence CMTSVATAKGYTSDL corresponding to internal region according to NP_002327.2.
• Specificity: This antibody is expected to recognise an epitope corresponding to aa 1546-1560 of human LRP6 protein.
• Predicted Species: Rat (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: LRP6
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 ug/mL
  • Immunohistochemistry 4 - 6 ug/mL
  • Immunohistochemistry-Paraffin 4 - 6 ug/mL
  • Peptide ELISA Detection limit 1:64000
  • Western Blot
• Application Notes: Use in Western blot reported in scientific literature (PMID: 20303298).
• Control:

  LRP-6 Overexpression Lysate

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 20303298).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for LRP-6 Antibody

• ADCAD2
• FLJ90062
• FLJ90421
• low density lipoprotein receptor-related protein 6
• low-density lipoprotein receptor-related protein 6
• LRP6
• LRP-6

Background

LRP6 is essential for the Wnt/beta catenin signaling pathway, probably by acting as a coreceptor together with Frizzled for Wnt. It is a specific, high-affinity receptor for DKK1 and DKK2, but not DKK3. The interaction with DKK1 blocks LRP6-mediated Wnt/beta catenin signaling via LRP6 removal via Kremen proteins-mediated endocytosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for LRP-6 Antibody (NB100-1475)(3)

Publications using NB100-1475

• Nava Porfirio, Koch Stefan, Laukoetter Mike G et al. Interferon-gamma regulates intestinal epithelial homeostasis through converging beta-catenin signaling pathways. Immunity 2010-01-01 [PMID: 20303298] (WB, Mouse)
• Liu W, Mani S, Davis NR et al. Mutation in EGFP Domain of LDL Receptor-Related Protein 6 Impairs Cellular LDL Clearance. Circ Res;103(11):1280-1288. 2008-01-01 [PMID: 18948618]
• Tamai K, Semenov M, Kato Y et al. LDL-receptor-related proteins in Wnt signal transduction. Nature 2000-09-28 [PMID: 11029007]

Bioinformatics

• Gene Symbol: LRP6
• Entrez:
  • 4040(Human)
  • 16974(Mouse)
• Uniprot:
  • O75581()