LRP-6 Antibodies

MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B)." class="big_lightbox"> MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B)." alt="MDA-MB-231 human breast cancer cell line was stained with Mouse Anti-Human LRP-6 Monoclonal Antibody (Catalog # MAB1505, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B)." class="big_thumb" />

NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox"> NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

HAF109). A specific band was detected for LRP-6 at approximately 180 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF109). A specific band was detected for LRP-6 at approximately 180 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of 293T human embryonic kidney cell line either mock transfected or transfected with human LRP-6. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human LRP-6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1505) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for LRP-6 at approximately 180 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

5036-WNP) for 15 minutes. PVDF Membrane was probed with 1 µg/mL of Rabbit Anti-Human Phospho-LRP-6 (S1490) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6649) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-LRP-6 (S1490) at approximately 200 kDa (as indicated). The phospho-specificity of this antibody was supported by decreased labeling following treatment with 300 U/mL CIP for 1 hour. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> 5036-WNP) for 15 minutes. PVDF Membrane was probed with 1 µg/mL of Rabbit Anti-Human Phospho-LRP-6 (S1490) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6649) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-LRP-6 (S1490) at approximately 200 kDa (as indicated). The phospho-specificity of this antibody was supported by decreased labeling following treatment with 300 U/mL CIP for 1 hour. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of 293T human embryonic kidney cell line transfected with human LRP-6 untreated (-) or treated (+) with 100 ng/mL Recombinant Human Wnt-3a (High Purity) (Catalog # 5036-WNP) for 15 minutes. PVDF Membrane was probed with 1 µg/mL of Rabbit Anti-Human Phospho-LRP-6 (S1490) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6649) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-LRP-6 (S1490) at approximately 200 kDa (as indicated). The phospho-specificity of this antibody was supported by decreased labeling following treatment with 300 U/mL CIP for 1 hour. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />