LRP-6 Overexpression Lysate (Native)

Images

 
Western Blot: LRP6 Overexpression Lysate (Adult Normal) [NBL1-12672] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for LRP6.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

LRP-6 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC218918 and based on accession number NM_002336. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens low density lipoprotein receptor-related protein 6 (LRP6), mRNA.
Gene
LRP6

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
178.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NBL1-12672 in the following application:

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for LRP-6

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for LRP-6 Overexpression Lysate (Native)

  • ADCAD2
  • FLJ90062
  • FLJ90421
  • low density lipoprotein receptor-related protein 6
  • low-density lipoprotein receptor-related protein 6
  • LRP6
  • LRP-6

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for LRP-6 Lysate (NBL1-12672) (0)

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Review for LRP-6 Lysate (NBL1-12672) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NBL1-12672:
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Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
WB Human 02/11/2015
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Summary

ApplicationWestern Blot
Sample TestedHEK293 whole cell lysate
SpeciesHuman

Blocking

Blocking DetailsSuperBlock (PBS) Blocking Buffer, 10ml per blot, room tempreture for 10mins

Details

Detection NotesChemi-luminescent,Bio rad imager, Exposure- 5min, positive cnt: Novus LRP6 overexpression lysate, Negetive cnt-HEK293cell lysate

Comments

Comments5ul of Over expression lysate is more than enough as a positive control

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for LRP-6 Lysate (NBL1-12672) (0)

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Recent Reviews

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Anonymous
02/11/2015
Application: WB
Species: Human

Bioinformatics

Gene Symbol LRP6
Entrez