LRP-6 [p Ser1490] Antibody [Unconjugated]

Western blot shows lysates of 293T human embryonic kidney cell line transfected with human LRP-6 untreated (-) or treated (+) with 100 ng/mL Recombinant Human Wnt-3a (High Purity) (Catalog # 5036-WNP) for 15 minutes. PVDF Membrane was probed with 1 µg/mL of Rabbit Anti-Human Phospho-LRP-6 (S1490) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6649) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-LRP-6 (S1490) at approximately 200 kDa (as indicated). The phospho-specificity of this antibody was supported by decreased labeling following treatment with 300 U/mL CIP for 1 hour. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: LYOPH

LRP-6 [p Ser1490] Antibody [Unconjugated] Summary

• Immunogen: Phosphopeptide containing the human LRP-6 S1490 site.
• Modification: p Ser1490
• Specificity: Detects human LRP-6 when phosphorylated at S1490 in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: LRP6
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS and Sodium Azide with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: Sodium Azide
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for LRP-6 [p Ser1490] Antibody [Unconjugated]

• ADCAD2
• FLJ90062
• FLJ90421
• low density lipoprotein receptor-related protein 6
• low-density lipoprotein receptor-related protein 6
• LRP6
• LRP-6

Background

LRP6 (Low-density lipoprotein receptor-related protein 6) is a 200-210 kDa member of the LDLR family of proteins. It is widely expressed, and serves as a coreceptor for both Wnt and parathyroid hormone. In the Wnt system, LRP6 associates with select Fzd multipass receptors; in the PTH system, LRP6 complexes with PTHR1. Mature human LRP6 is a 1594 amino acid (aa) type I transmembrane glycoprotein. It contains a 1351 aa extracellular region (aa 20-1370) plus a 220 aa cytoplasmic domain (aa 1394-1613). The cytoplasmic domain contains two palmitoylation sites, one ubiquitination residue, and multiple phosphorylation motifs. Ser1490 is a key residue that impacts multiple activities. It undergoes both constitutive and receptor activation-induced phosphorylation. At least three enzymes likely act at this site, including PKA (associated with PTHR1), GSK-3 (associated with Fzd) and Cyclin Y/PFTK1 (associated with G2/M of the cell cycle). In general, Ser1490 phosphorylation results in beta -catenin stabilization, followed by either gene transcription or beta -catenin binding to centrosomes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for LRP-6 Antibody (AF6649)(2)

Publications using AF6649

• G Riitano, A Capozzi, S Recalchi, D Caissutti, A Longo, V Mattei, F Conti, R Misasi, T Garofalo, M Sorice, V Manganelli Anti-beta2-GPI Antibodies Induce Endothelial Cell Expression of Tissue Factor by LRP6 Signal Transduction Pathway Involving Lipid Rafts Cells, 2022-04-11;11(8):. 2022-04-11 [PMID: 35455968] (Western Blot, Human)
• Thrasivoulou C, Millar M, Ahmed A Activation of intracellular calcium by multiple Wnt ligands and translocation of beta-catenin into the nucleus: a convergent model of Wnt/Ca2+ and Wnt/beta-catenin pathways. J Biol Chem, 2013-10-24;288(50):35651-9. 2013-10-24 [PMID: 24158438] (Western Blot, Human)

Bioinformatics

• Gene Symbol: LRP6