LRP-6 Antibody

Images

 
Western blot shows lysates of 293T human embryonic kidney cell line either mock transfected or transfected with human LRP‑6. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human LRP‑6 Antigen Affinity-purified ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, CyTOF-ready
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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LRP-6 Antibody Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human LRP-6
Ala20-Pro1368
Accession # O75581
Specificity
Detects human LRP-6 in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross-reactivity with recombinant mouse LRP-6 is observed, and less than 1% cross-reactivity with recombinant human (rh) LRP-1, rhLRP-4, and rhLRP-5 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
LRP6
Purity
Immunogen affinity purified
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Western Blot 0.1 ug/mL
Publications
Read Publications using
AF1505 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for LRP-6 Antibody

  • ADCAD2
  • FLJ90062
  • FLJ90421
  • low density lipoprotein receptor-related protein 6
  • low-density lipoprotein receptor-related protein 6
  • LRP6
  • LRP-6

Background

The low-density lipoprotein (LDL) receptor-related protein 5 (LRP-5) and LRP-6 constitute a distinct subgroup of the LDL receptor family. Both LRP-5 and LRP-6 are type I transmembrane proteins that function as co-receptors with Frizzled (FZD) in the canonical Wnt signaling pathway (1, 2). LRP-6 cDNA encodes a 1613 amino acid residue (aa) precursor with a 19 aa signal sequence, a 1353 aa extracellular region, a 23 aa transmembrane (TM) segment and a 218 aa cytoplasmic domain (3). The extracellular region contains four N-terminal cysteine-rich EGF-like repeats, followed by three cysteine-rich LDLR repeats. This pattern of the EGF and LDLR repeat arrangement is different than that typically found in other LDL receptor family proteins. The intracellular region of LRP6 contains protein-protein interaction motifs and is required for canonical Wnt signal transduction (4). Human LRP-6 shares 98% and 74% aa sequence identity with mouse LRP-6 and human LRP-5, respectively. Based on the current model, canonical Wnt signaling requires the interaction of Wnt with FZD and LRP to form a trimeric complex which signals downstream to stabilize cytoplasmic  beta -catenin. The stabilized beta -catenin is then translocated to the nucleus where it complexes with the transcription factor LEF/TCF to regulate the transcription of target genes (5). LRP-6 has also been shown to interact with the Dickkopf proteins (DKKs), which are modulators of Wnt signaling (6-8). Binding of DKK-1 to LRP-6 dissociates LRP-6 from FZD, and antagonizes the formation of the functional receptor complex. On cells where the transmembrane proteins Kremens are also present, a ternary complex of LRP-6, DKK-1 and Kremen is formed to trigger the internalization of the complex and removal LRP6 from the cell surface. Thus, DKK-1 and Kremen function synergistically to antagonize LRP-6-mediated Wnt activity. Although DKK-2 also functions as a Wnt antagonist on cells that express Kremen, DKK-2 binding to LRP-6 in the absence of Kremen activates rather than inhibits LRP mediated beta -catenin signaling (9, 10).

  1. Howell, B.W. and J. Herz (2001) Curr. Op. Neurobiology 11:74.
  2. Pinson, K.I. et al. (2000) Nature 407:535.
  3. Brown, S.D. et al. (1998) Biochem. Biophys. Res. Commun. 248:879.
  4. Tamai, K. et al. (2000) Nature 407:530.
  5. Schweizer, L. and H. Varmus (2003) BMC Cell Biology 4:4.
  6. Mao, B. et al. (2001) Nature 411:321.
  7. Semenov, M.V. et al. (2001) Curr. Biol. 11:951.
  8. Bafico, A. et al. (2001) Nature Cell Biol. 3:683.
  9. Zorn, A.M. (2001) Curr. Biol. 11:R592.
  10. Mao, B. et al. (2002) Nature 417:664.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for LRP-6 Antibody (AF1505)(5)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: ICC, Immunoprecipitation, Western Blot.


Filter By Application
ICC
(1)
Immunoprecipitation
(1)
Western Blot
(3)
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Human
(3)
Mouse
(2)
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Showing Publications 1 - 5 of 5.
Publications using AF1505 Applications Species
G Riitano, A Capozzi, S Recalchi, D Caissutti, A Longo, V Mattei, F Conti, R Misasi, T Garofalo, M Sorice, V Manganelli Anti-beta2-GPI Antibodies Induce Endothelial Cell Expression of Tissue Factor by LRP6 Signal Transduction Pathway Involving Lipid Rafts Cells, 2022-04-11;11(8):. 2022-04-11 [PMID: 35455968] (Immunoprecipitation, Human) Immunoprecipitation Human
RS Priestley, J Cheung, EJ Murphy, MT Ehebauer, JB Davis, E Di Daniel A novel high-content imaging-based technique for measuring binding of Dickkopf-1 to low-density lipoprotein receptor-related protein 6 J Pharmacol Toxicol Methods, 2018-11-23;0(0):. 2018-11-23 [PMID: 30476623] (ICC, Human) ICC Human
Grunberg J, Hammarstedt A, Hedjazifar S, Smith U The Novel Secreted Adipokine WNT1-inducible Signaling Pathway Protein 2 (WISP2) Is a Mesenchymal Cell Activator of Canonical WNT. J Biol Chem, 2014-01-22;289(10):6899-907. 2014-01-22 [PMID: 24451367] (Western Blot, Mouse) Western Blot Mouse
Gustafson B, Smith U Activation of canonical wingless-type MMTV integration site family (Wnt) signaling in mature adipocytes increases beta-catenin levels and leads to cell dedifferentiation and insulin resistance. J. Biol. Chem., 2010-02-23;285(18):14031-41. 2010-02-23 [PMID: 20179324] (Western Blot, Mouse) Western Blot Mouse
Liu CC, Pearson C, Bu G Cooperative folding and ligand-binding properties of LRP6 beta-propeller domains. J. Biol. Chem., 2009-03-31;284(22):15299-307. 2009-03-31 [PMID: 19339249] (Western Blot, Human) Western Blot Human

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Bioinformatics

Gene Symbol LRP6
Uniprot