LRH-1/NR5A2 Antibody (H2325) [Unconjugated]

Effects of loss of LRH-1 on murine organoids. a Expression of mLRH-1 transcripts in intestinal organoids is widespread and higher in crypt regions (dashed black line). Scale bar = 100 μm. b Loss of mLRH-1 after Cre-recombination induced by 4-hydroxytamoxifen (4OHT) added to Lrh-1fl/fl;Vil-CreERT2 organoids for 48 h (Lrh1IEC-KO) compared to similarly treated wild-type (Lrh1fl/fl) organoids, as detected by anti-LRH-1 antibody. c Cell death in Lrh1fl/fl and Lrh1IEC-KO organoids following acute loss of Lrh-1. Values normalized to five independent wells of untreated Lrh1fl/fl organoids taken to be 0%. d Most significant gene changes up (blue) or down (ochre) after loss of mLRH-1 by RNA expression (q = < 0.05 and p = < 0.005). Gene names in bold refer to markers of differentiation while red refer to known EEC markers. e Top five altered gene networks as identified by IPA Ingenuity analysis. fLrh1fl/fl and Lrh1IEC-KO organoids exposed to TNF alpha (10 ng/ml) for 40 h stained for active Casp3 (red) and CD44 (green). Cells expressing active Casp3 undergoing cell death in crypt regions are indicated (white arrowheads and dashed white line). Scale bar = 50 μm. g Expression of active Casp3 in Lrh1fl/fl and Lrh1IEC-KO organoids as described in panel f, detected by western blotting (17 and19 kDa, black arrowheads). Shorter (top panel) and longer exposures (middle panel). h Uptake of fluorescent dextran after 30 min in Lrh1fl/fl and Lrh1IEC-KO organoids. Scale bar = 100 μm. Bar graph of percentage dye-positive enteroids (≥30 total organoids counted per condition). Data represent average of minimum three biological triplicates. For c and g, error bars are SEM using Student’s t test (unpaired, two tailed) with p values of ****p = < 0.0001 Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/30305617), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Hu
• Applications: WB, IP, ELISA
• Clone: H2325
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Suitable Sample Type: 23015

LRH-1/NR5A2 Antibody (H2325) [Unconjugated] Summary

• Immunogen: Recombinant human LRH-1/NR5A2
  aa 161-280
• Specificity: This antibody specifically recognizes human LRH-1 alpha and LRH-1 beta and cross-reacts with mouse and rat LRH-1. Not yet tested in other species.
• Source: N/A
• Isotype: IgG3
• Clonality: Monoclonal
• Host: Mouse
• Gene: NR5A2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Direct ELISA
  • Immunoprecipitation
  • Western Blot

Packaging, Storage & Formulations

• Storage: This antibody is stable for greater than six months when held at -20 °C in a manual defrost freezer or at -70 °C. Upon thawing, the antibody can be stored at 2-8 °C for at least 1 month without detectable loss of activity. Avoid repeated freeze-thaw cycles.
• Buffer: A liquid formulation in physiologic saline with 0.1% NaN₃.
• Preservative: 0.1% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for LRH-1/NR5A2 Antibody (H2325) [Unconjugated]

• Alpha-1-fetoprotein transcription factor
• B1-binding factor
• b1-binding factor, hepatocyte transcription factor which activates enhancer IIof hepatitis B virus
• B1F
• B1F2
• fetoprotein-alpha 1 (AFP) transcription factor
• FTFLRH1
• FTZ-F1 beta
• FTZ-F1
• FTZ-F1beta
• HB1F
• hB1F-2
• hB1FCYP7A promoter-binding factor
• Hepatocytic transcription factor
• Liver receptor homolog 1
• liver receptor homolog-1
• LRH1
• LRH-1
• LRH-1CPF
• NR5A2
• nuclear receptor NR5A2
• nuclear receptor subfamily 5 group A member 2
• nuclear receptor subfamily 5, group A, member 2

Background

Liver Receptor Homologous Protein (LRH-1, FTF, CPF, hB1F, FTZ-F1b; NR5A2) is a member of the Orphan Nuclear Receptor family. LRH-1 is a mammalian homolog of the Drosophila Fushi Tarazu F1 Gene product. LRH-1 is expressed in the liver and intestine. LRH-1 is involved in the regulation of genes which participate in steroid, bile acid and cholesterol homeostasis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for LRH-1/NR5A2 Antibody (PP-H2325-00)(16)

Publications using PP-H2325-00

• Nguyen, NMP;Chang, EM;Chauvin, M;Sicher, N;Kashiwagi, A;Nagykery, N;Chow, C;May, P;Mermin-Bunnel, A;Cleverdon, J;Duong, T;Meinsohn, MC;Gao, D;Donahoe, PK;Pepin, D; AMH protects the ovary from doxorubicin by regulating cell fate and the response to DNA damage bioRxiv : the preprint server for biology 2024-05-23 [PMID: 38826466] (Immunocytochemistry, Mouse)
• Birgersson, M;Indukuri, R;Lindquist, L;Stepanauskaite, L;Luo, Q;Deng, Q;Archer, A;Williams, C; Ovarian ER? cistrome and transcriptome reveal chromatin interaction with LRH-1 BMC biology 2023-11-29 [PMID: 38031019] (ChIP, Mouse)
• J Son, W Du, M Esposito, K Shariati, H Ding, Y Kang, D Accili Genetic and pharmacologic inhibition of ALDH1A3 as a treatment of beta-cell failure Nature Communications, 2023-02-02;14(1):558. 2023-02-02 [PMID: 36732513] (IHC, Mouse)
• Yu Sun, Hadrien Demagny, Adrien Faure, Francesca Pontanari, Antoine Jalil, Nadia Bresciani et al. Asparagine protects pericentral hepatocytes during acute liver injury Journal of Clinical Investigation 2023-04-03 [PMID: 36719750]
• KP Kim, Y Wu, J Yoon, K Adachi, G Wu, S Velychko, CM MacCarthy, B Shin, A Röpke, MJ Arauzo-Bra, M Stehling, DW Han, Y Gao, J Kim, S Gao, HR Schöler Reprogramming competence of OCT factors is determined by transactivation domains Science Advances, 2020-09-02;6(36):. 2020-09-02 [PMID: 32917606] (Western Blot, Mouse)
• Corey D. Seacrist, Georg Kuenze, Reece M. Hoffmann, Brandon E. Moeller, John E. Burke, Jens Meiler et al. Integrated Structural Modeling of Full-Length LRH-1 Reveals Inter-domain Interactions Contribute to Receptor Structure and Function Structure 2020-07-01 [PMID: 32433991]
• Corey D. Seacrist, Georg Kuenze, Reece M. Hoffmann, Brandon E. Moeller, John E. Burke, Jens Meiler et al. Integrated Structural Modeling of Full-Length LRH-1 Reveals Inter-domain Interactions Contribute to Receptor Structure and Function Structure 2020-07-01 [PMID: 32433991] (Western Blot, Human)
• A Milona, V Massafra, H Vos, J Naik, N Artigas, HAB Paterson, ITGW Bijsmans, ECL Willemsen, JM Ramos Pitt, I Miguel-Ali, P Bosma, BMT Burgering, C Williamson, S Vernia, WS Dhillo, SWC van Mil, BM Owen Steroidogenic control of liver metabolism through a nuclear receptor-network Mol Metab, 2019-09-30;30(0):221-229. 2019-09-30 [PMID: 31767173] (ChIP, Mouse)
• JR Bayrer, H Wang, R Nattiv, M Suzawa, HS Escusa, RJ Fletterick, OD Klein, DD Moore, HA Ingraham LRH-1 mitigates intestinal inflammatory disease by maintaining epithelial homeostasis and cell survival Nat Commun, 2018-10-10;9(1):4055. 2018-10-10 [PMID: 30305617] (Western Blot, Human)
• A Zou, N Magee, F Deng, S Lehn, C Zhong, Y Zhang Hepatocyte nuclear receptor SHP suppresses inflammation and fibrosis in a mouse model of nonalcoholic steatohepatitis J. Biol. Chem., 2018-04-17;0(0):. 2018-04-17 [PMID: 29666185] (Immunoprecipitation, Human)
• DA Miranda, WC Krause, A Cazenave-G, M Suzawa, H Escusa, JC Foo, DS Shihadih, A Stahl, M Fitch, E Nyangau, M Hellerstei, MR Wenk, DL Silver, HA Ingraham LRH-1 regulates hepatic lipid homeostasis and maintains arachidonoyl phospholipid pools critical for phospholipid diversity JCI Insight, 2018-03-08;3(5):. 2018-03-08 [PMID: 29515023] (Western Blot, Mouse)
• Martin Wagner, Sungwoo Choi, Katrin Panzitt, Jennifer L. Mamrosh, Jae Man Lee, Alex Zaufel et al. Liver receptor homolog‐1 is a critical determinant of methyl‐pool metabolism Hepatology 2016-01-01 [PMID: 26267291]
• Bayrer J, Mukkamala S, Sablin E, Webb P, Fletterick R Silencing LRH-1 in colon cancer cell lines impairs proliferation and alters gene expression programs. Proc Natl Acad Sci U S A, 2015-02-09;112(8):2467-72. 2015-02-09 [PMID: 25675535] (Western Blot, Human)
• Hansook Kim Chong, Jacob Biesinger, Young-Kyo Seo, Xiaohui Xie, Timothy F Osborne Genome-wide analysis of hepatic LRH-1 reveals a promoter binding preference and suggests a role in regulating genes of lipid metabolism in concert with FXR BMC Genomics 2012-12-01 [PMID: 22296850]
• Holmstrom SR, Deering T, Swift GH LRH-1 and PTF1-L coregulate an exocrine pancreas-specific transcriptional network for digestive function. Genes Dev., 2011-08-15;25(16):1674-9. 2011-08-15 [PMID: 21852532] (ChIP, Mouse)
• Guo G, Smith A A genome-wide screen in EpiSCs identifies Nr5a nuclear receptors as potent inducers of ground state pluripotency. Development, 2010-10-01;137(19):3185-92. 2010-10-01 [PMID: 20823062] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: NR5A2