LRG1 Antibody

Images

 
Western blot shows human plasma. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Human LRG1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7890) followed by HRP-conjugated Anti-Sheep IgG Secondary ...read more
LRG1 was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Sheep Anti-Human LRG1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7890) at 3 µg/mL overnight at 4 °C. Tissue ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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LRG1 Antibody Summary

Immunogen
Human embryonic kidney cell line HEK293-derived recombinant human LRG1
Val36-Gln347
Accession # P02750
Specificity
Detects human LRG1 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
LRG1
Purity
Immunogen affinity purified
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 2 ug/mL
Publications
Read Publication using
AF7890 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for LRG1 Antibody

  • 1300008B03Rik
  • 2310031E04Rik
  • HMFT1766
  • leucine-rich alpha-2-glycoprotein 1
  • leucine-rich alpha-2-glycoprotein
  • LRG1
  • LRGleucine rich alpha 2 glycoprotein

Background

LRG-1 (Leucine-Rich alpha-2-Glycoprotein 1; also LRHG [in rodent]) is a 45-55 kDa, monomeric, variably glycosylated, secreted protein member of the leucine-rich repeat family of molecules. It should not be confused with either LRIG-1/ Leucine-Rich repeats and Ig-like domains-1, or LRG1/LIM-RhoGap protein 1. LRG-1 has a limited expression pattern, being associated with neutrophils, neutrophil precursors (myelocytes; metamyelocytes), astrocytes, HEV (in mice), hepatocytes and select tumor cells. LRG-1 is reported to bind multiple molecules, including TGF-beta , extracellular cytochrome C, and fibronectin plus collagen IV of the ECM. Functions associated with this binding include granulocyte maturation, protection from apoptosis, and cell migration. Mature human LRG-1 is 312 amino acids (aa) in length (aa 35-347). Depending upon the source, LRG-1 has been described as having either thirteen 24 aa leucine-rich repeats (LRRs), or eight LRRs spanning aa 93-282 coupled to a C-terminal LRR. Variable O- and N-based glycosylation accounts for the variablility in MW. Mature human LRG-1 shares 66% aa sequence identity with mouse LRG-1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for LRG1 Antibody (AF7890)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: ELISA (Capture).


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ELISA (Capture)
(1)
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Product General Protocols

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Video Protocols

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

PE NBP3-12110PE

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Bioinformatics

Gene Symbol LRG1
Uniprot