LOX-1/OLR1 Antibody [Unconjugated]

LOX-1/OLR1 was detected in immersion fixed paraffin-embedded sections of human placenta using Goat Anti-Human LOX-1/OLR1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1798) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytotrophoblasts. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Generation of stable prostate cancer cell lines with LOX-1 over-expression and shRNA against olr1.A) Western blot for LOX-1 (40 kDa) expression in human CaP clones with overexpression of LOX-1. B) Western blot for LOX-1 (40 kDa) expression in human prostate cancer cell clones with LOX-1 knockdown C) Real-time PCR for LOX-1 expression in three clones with overexpression of LOX-1. D) Real-time PCR for LOX-1 expression was determined in three clones that express shRNA/LOX-1(A), and three clones that express shRNA/LOX-1(B). The data represent the means ± S.D. of three independent experiments performed in triplicate, and statistically analyzed using one-way analysis of variance and Dunnett’s post-test; (***p≤0.001, **p≤0.01, *p≤0.05). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25170920), licensed under a CC-BY license. Not internally tested by R&D Systems.

Generation of stable prostate cancer cell lines with LOX-1 over-expression and shRNA against olr1.A) Western blot for LOX-1 (40 kDa) expression in human CaP clones with overexpression of LOX-1. B) Western blot for LOX-1 (40 kDa) expression in human prostate cancer cell clones with LOX-1 knockdown C) Real-time PCR for LOX-1 expression in three clones with overexpression of LOX-1. D) Real-time PCR for LOX-1 expression was determined in three clones that express shRNA/LOX-1(A), and three clones that express shRNA/LOX-1(B). The data represent the means ± S.D. of three independent experiments performed in triplicate, and statistically analyzed using one-way analysis of variance and Dunnett’s post-test; (***p≤0.001, **p≤0.01, *p≤0.05). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25170920), licensed under a CC-BY license. Not internally tested by R&D Systems.

Generation of stable prostate cancer cell lines with LOX-1 over-expression and shRNA against olr1.A) Western blot for LOX-1 (40 kDa) expression in human CaP clones with overexpression of LOX-1. B) Western blot for LOX-1 (40 kDa) expression in human prostate cancer cell clones with LOX-1 knockdown C) Real-time PCR for LOX-1 expression in three clones with overexpression of LOX-1. D) Real-time PCR for LOX-1 expression was determined in three clones that express shRNA/LOX-1(A), and three clones that express shRNA/LOX-1(B). The data represent the means ± S.D. of three independent experiments performed in triplicate, and statistically analyzed using one-way analysis of variance and Dunnett’s post-test; (***p≤0.001, **p≤0.01, *p≤0.05). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25170920), licensed under a CC-BY license. Not internally tested by R&D Systems.

Generation of stable prostate cancer cell lines with LOX-1 over-expression and shRNA against olr1.A) Western blot for LOX-1 (40 kDa) expression in human CaP clones with overexpression of LOX-1. B) Western blot for LOX-1 (40 kDa) expression in human prostate cancer cell clones with LOX-1 knockdown C) Real-time PCR for LOX-1 expression in three clones with overexpression of LOX-1. D) Real-time PCR for LOX-1 expression was determined in three clones that express shRNA/LOX-1(A), and three clones that express shRNA/LOX-1(B). The data represent the means ± S.D. of three independent experiments performed in triplicate, and statistically analyzed using one-way analysis of variance and Dunnett’s post-test; (***p≤0.001, **p≤0.01, *p≤0.05). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25170920), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Hu
• Applications: WB, IHC, B/N
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

LOX-1/OLR1 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human LOX-1 (R&D Systems, Catalog # 1798-LX)
  Ser61-Gln273
  Accession # P78380
• Specificity: Selected for its ability to block receptor-ligand interaction in a functional ELISA assay. In direct ELISAs, less than 5% cross‑reactivity with recombinant mouse LOX-1 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: OLR1
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Blockade of Receptor-ligand Interaction
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for LOX-1/OLR1 Antibody [Unconjugated]

• CLEC8A
• CLEC8ASLOX1
• C-type lectin domain family 8 member A
• hLOX-1
• Lectin-like oxidized LDL receptor 1
• Lectin-like oxLDL receptor 1
• Lectin-type oxidized LDL receptor 1
• LOX1
• LOX-1
• LOX1ox LDL receptor 1
• LOXIN
• OLR1
• oxidised low density lipoprotein (lectin-like) receptor 1
• oxidized low density lipoprotein (lectin-like) receptor 1
• oxidized low-density lipoprotein receptor 1
• oxidized low-density lipoprotein receptor 1, soluble form
• Ox-LDL receptor 1
• SCARE1
• scavenger receptor class E, member 1
• SR-E1

Background

Lectin-like oxidized low-density-lipoprotein receptor-1 (LOX-1), also known as oxidized low-density-lipoprotein receptor-1 (OLR-1), is a type II transmembrane receptor belonging to the C-type lectin family (1). It also belongs to the functionally defined scavenger receptor (SR) superfamily, whose members share the common ability to bind and internalize modified forms of Low Density Lipoproteins (LDL) (2 - 4). LOX-1 is the first member of the class E scavenger receptor subfamily (SR-E). It binds and supports the internalization of multiple structurally unrelated macromolecules including oxidized LDL, advanced glycation end products (AGE), activated platelets, bacteria, apoptotic or aged cells, and heat shock proteins (5 - 7). LOX-1 has also been implicated as an intestinal receptor involved in the transcytosis of pancreatic bile salt-dependent lipase (8). The human LOX-1 gene encodes a 273 amino acid (aa) residue protein with a short N-terminal intracellular domain, a transmembrane domain, an extracellular stalk/neck region followed by a C-type lectin-like domain (CTLD) (1, 6). The CTLD, which is required for ligand recognition, contains the six conserved cysteine residues present in all C-type lectins, but lacks the Ca²⁺-binding residues found in classical C-type lectins. LOX-1 can be detected on activated endothelial cells, vascular smooth muscle cells, macrophages, intestinal cells and dendritic cells (6 - 8). The expression of LOX-1 is induced by proinflammatory or proatherogenic stimuli, as well as by oxidized LDL itself and hemodynamic or oxidative stress. Human LOX-1 exists on the cell surface as covalent homodimers, which can further associate into non-covalent-linked oligomers (9). Cell surface LOX-1 can also be cleaved by yet unidentified proteases to release the soluble LOX-1 extracellular domain (6). Binding and endocytosis of oxidized LDL by LOX-1 induces oxidative stress, activates NF kappa B, and upregulates the expression of monocyte chemoattractant protein-1 and matrix metalloproteases (5 - 9). LOX-1-dependent oxidized LDL uptake also induces apoptosis by inducing the expression of the pro-apoptotic Bax and downregulation of the anti-apoptotic Bcl-2 (10). Oxidized LDL plays a key role in the pathogenesis of atherosclerosis and endothelial dysfunction. Blockade of LOX-1 functions may turn out to be a suitable target for the therapeutic intervention of atherosclerosis.

1. Sawamura, T. et al. (1997) Nature 386:73. 
2. Daugherty, A. et al. (2000) Curr. Opin. Cardiovasc. Pulm. Ren. Invest. Drugs. 2:223. 
3. Platt, N. and S. Gordon (2001) J. Clin. Invest. 108:649. 
4. Platt, N. and S. Gordon (1998) Chem. Biol. 5:R193. 
5. Jono, T. et al. (2002) FEBS Lett. 511:170. 
6. Kume, N. et al. (2001) Curr. Opin. Lipidol. 12:419.
7. Delneste, Y. et al. (2002) Immunity 17:353.
8. Bruneau, N. et al. (2003) Mol. Biol. Cell 14:2861.
9. Xie, Q. et al. (2004) DNA and Cell Biol. 23:111.
10. Chen, J. et al. (2003) Circ. Res. 94:370.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for LOX-1/OLR1 Antibody (AF1798)(8)

Publications using AF1798

• Florida, EM;Li, H;Hong, CG;Ongstad, EL;Gaddipati, R;Sitaula, S;Varma, V;Parel, PM;O'Hagan, R;Chen, MY;Teague, HL;Playford, MP;Karathanasis, SK;Collén, A;Mehta, NN;Remaley, AT;Sorokin, AV; Relationship of Soluble Lectin-Like Low-Density Lipoprotein Receptor-1 (sLOX-1) With Inflammation and Coronary Plaque Progression in Psoriasis Journal of the American Heart Association 2023-11-20 [PMID: 37982276] (ELISA Detection, Human)
• Yoshinori Aoki, Hongmei Dai, Fumika Furuta, Tomohisa Akamatsu, Takuya Oshima, Naoto Takahashi et al. LOX-1 mediates inflammatory activation of microglial cells through the p38-MAPK/NF-kappa B pathways under hypoxic-ischemic conditions Cell Communication and Signaling 2023-06-02 [PMID: 37268943]
• D Sagar, R Gaddipati, EL Ongstad, N Bhagroo, LL An, J Wang, M Belkhodja, S Rahman, Z Manna, MA Davis, S Hasni, R Siegel, M Sanjuan, J Grimsby, R Kolbeck, S Karathanas, GP Sims, R Gupta LOX-1: A potential driver of cardiovascular risk in SLE patients PLoS ONE, 2020-03-17;15(3):e0229184. 2020-03-17 [PMID: 32182251] (ELISA Detection, Human)
• G Zhao, M Hu, C Li, J Lee, K Yuan, G Zhu, C Che Osteopontin contributes to effective neutrophils recruitment, IL-1? production, and apoptosis in Aspergillus fumigatus keratitis Immunol. Cell Biol., 2018-02-26;0(0):. 2018-02-26 [PMID: 29359350] (Neutralization, Human)
• Gonzalez-Chavarria I, Cerro R, Parra N, Sandoval F, Zuniga F, Omazabal V, Lamperti L, Jimenez S, Fernandez E, Gutierrez N, Rodriguez F, Onate S, Sanchez O, Vera J, Toledo J Lectin-like oxidized LDL receptor-1 is an enhancer of tumor angiogenesis in human prostate cancer cells. PLoS ONE, 2014-08-29;9(8):e106219. 2014-08-29 [PMID: 25170920] (ICC, Western Blot, Human)
• Valente AJ, Irimpen AM, Siebenlist U et al. OxLDL induces endothelial dysfunction and death via TRAF3IP2: inhibition by HDL3 and AMPK activators Free Radic Biol Med 2/25/2014 [PMID: 24561578]
• Stephen J. White, Graciela B. Sala-Newby, Andrew C. Newby Overexpression of scavenger receptor LOX-1 in endothelial cells promotes atherogenesis in the ApoE(-/-) mouse model Cardiovascular Pathology 2011-11-01 [PMID: 20943418]
• Goette A, Bukowska A, Dobrev D, Pfeiffenberger J, Morawietz H, Strugala D, Wiswedel I, Rohl FW, Wolke C, Bergmann S, Bramlage P, Ravens U, Lendeckel U Acute atrial tachyarrhythmia induces angiotensin II type 1 receptor-mediated oxidative stress and microvascular flow abnormalities in the ventricles. Eur. Heart J., 2009-03-05;30(11):1411-20. 2009-03-05 [PMID: 19269986] (Western Blot, Porcine)

Review for LOX-1/OLR1 Antibody (AF1798) (1)

Average Rating: 3

(Based on 1 review)

We have 1 review tested in 1 species: Other.

reviewed by: Verified Customer WB Other 01/05/2015

Summary

• Application: Western Blot
• Sample Tested: See PMID 23950953
• Species: Other

Bioinformatics

• Gene Symbol: OLR1
• Uniprot:
  • P78380()