LOX-1/OLR1 Antibody [Unconjugated]

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LOX‑1/OLR1 was detected in immersion fixed paraffin-embedded sections of human placenta using Goat Anti-Human LOX‑1/OLR1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1798) at 1 µg/mL overnight at 4 ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, B/N
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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LOX-1/OLR1 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human LOX-1 (R&D Systems, Catalog # 1798-LX)
Ser61-Gln273
Accession # P78380
Specificity
Selected for its ability to block receptor-ligand interaction in a functional ELISA assay. In direct ELISAs, less than 5% cross‑reactivity with recombinant mouse LOX-1 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
OLR1
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Blockade of Receptor-ligand Interaction
Control
LOX-1/OLR1 Knockout HeLa Cell Lysate
Reviewed Applications
Read 1 Review rated 3
using
AF1798 in the following applications:

Publications
Read Publications using
AF1798 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for LOX-1/OLR1 Antibody [Unconjugated]

  • CLEC8A
  • CLEC8ASLOX1
  • C-type lectin domain family 8 member A
  • hLOX-1
  • Lectin-like oxidized LDL receptor 1
  • Lectin-like oxLDL receptor 1
  • Lectin-type oxidized LDL receptor 1
  • LOX1
  • LOX-1
  • LOX1ox LDL receptor 1
  • LOXIN
  • OLR1
  • oxidised low density lipoprotein (lectin-like) receptor 1
  • oxidized low density lipoprotein (lectin-like) receptor 1
  • oxidized low-density lipoprotein receptor 1
  • oxidized low-density lipoprotein receptor 1, soluble form
  • Ox-LDL receptor 1
  • SCARE1
  • scavenger receptor class E, member 1
  • SR-E1

Background

Lectin-like oxidized low-density-lipoprotein receptor-1 (LOX-1), also known as oxidized low-density-lipoprotein receptor-1 (OLR-1), is a type II transmembrane receptor belonging to the C-type lectin family (1). It also belongs to the functionally defined scavenger receptor (SR) superfamily, whose members share the common ability to bind and internalize modified forms of Low Density Lipoproteins (LDL) (2 - 4). LOX-1 is the first member of the class E scavenger receptor subfamily (SR-E). It binds and supports the internalization of multiple structurally unrelated macromolecules including oxidized LDL, advanced glycation end products (AGE), activated platelets, bacteria, apoptotic or aged cells, and heat shock proteins (5 - 7). LOX-1 has also been implicated as an intestinal receptor involved in the transcytosis of pancreatic bile salt-dependent lipase (8). The human LOX-1 gene encodes a 273 amino acid (aa) residue protein with a short N-terminal intracellular domain, a transmembrane domain, an extracellular stalk/neck region followed by a C-type lectin-like domain (CTLD) (1, 6). The CTLD, which is required for ligand recognition, contains the six conserved cysteine residues present in all C-type lectins, but lacks the Ca2+-binding residues found in classical C-type lectins. LOX-1 can be detected on activated endothelial cells, vascular smooth muscle cells, macrophages, intestinal cells and dendritic cells (6 - 8). The expression of LOX-1 is induced by proinflammatory or proatherogenic stimuli, as well as by oxidized LDL itself and hemodynamic or oxidative stress. Human LOX-1 exists on the cell surface as covalent homodimers, which can further associate into non-covalent-linked oligomers (9). Cell surface LOX-1 can also be cleaved by yet unidentified proteases to release the soluble LOX-1 extracellular domain (6). Binding and endocytosis of oxidized LDL by LOX-1 induces oxidative stress, activates NF kappa B, and upregulates the expression of monocyte chemoattractant protein-1 and matrix metalloproteases (5 - 9). LOX-1-dependent oxidized LDL uptake also induces apoptosis by inducing the expression of the pro-apoptotic Bax and downregulation of the anti-apoptotic Bcl-2 (10). Oxidized LDL plays a key role in the pathogenesis of atherosclerosis and endothelial dysfunction. Blockade of LOX-1 functions may turn out to be a suitable target for the therapeutic intervention of atherosclerosis.

  1. Sawamura, T. et al. (1997) Nature 386:73. 
  2. Daugherty, A. et al. (2000) Curr. Opin. Cardiovasc. Pulm. Ren. Invest. Drugs. 2:223. 
  3. Platt, N. and S. Gordon (2001) J. Clin. Invest. 108:649. 
  4. Platt, N. and S. Gordon (1998) Chem. Biol. 5:R193. 
  5. Jono, T. et al. (2002) FEBS Lett. 511:170. 
  6. Kume, N. et al. (2001) Curr. Opin. Lipidol. 12:419.
  7. Delneste, Y. et al. (2002) Immunity 17:353.
  8. Bruneau, N. et al. (2003) Mol. Biol. Cell 14:2861.
  9. Xie, Q. et al. (2004) DNA and Cell Biol. 23:111.
  10. Chen, J. et al. (2003) Circ. Res. 94:370.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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AF1798
Species: Hu
Applications: WB, IHC, B/N

Publications for LOX-1/OLR1 Antibody (AF1798)(3)

Review for LOX-1/OLR1 Antibody (AF1798) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Other.

Reviews using AF1798:
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(1)
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reviewed by:
Anonymous
WB Other 01/05/2015
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Summary

ApplicationWestern Blot
Sample TestedSee PMID 23950953
SpeciesOther

Blocking

Blocking DetailsSee PMID 23950953

Details

Detection NotesSee PMID 23950953

Comments

CommentsPublished: See PMID 23950953

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Control Lysate(s)

Secondary Antibodies

 

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Other Available Formats

Biotin BAF1798

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Anonymous
01/05/2015
Application: WB
Species: Other

Bioinformatics

Gene Symbol OLR1
Uniprot