LOK Antibody

Western Blot: LOK Antibody [NBP2-19368] - Wild-type (WT) and LOK knockout (KO) HeLa cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with LOK antibody [N1N2], N-term. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: LOK Antibody [NBP2-19368] - Sample (30 ug of whole cell lysate) A: A431 5% SDS PAGE gel, diluted at 1:2000.

Immunocytochemistry/Immunofluorescence: LOK Antibody [NBP2-19368] - Analysis of methanol-fixed A431, using antibody at 1:500 dilution.

Immunohistochemistry-Paraffin: LOK Antibody [NBP2-19368] - Human breast cancer, using LOK antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC, KO
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

LOK Antibody Summary

• Description: Novus Biologicals Knockout (KO) Validated Rabbit LOK Antibody (NBP2-19368) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human LOK. The exact sequence is proprietary.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: STK10
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Knockout Validated
  • Western Blot 1:500-1:3000
• Theoretical MW: 112 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Xenopus laevis (84%), Zebrafish (82%), Xenopus tropicalis (84%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol, 1% BSA
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for LOK Antibody

• EC 2.7.11
• EC 2.7.11.1
• LOKserine/threonine-protein kinase 10
• Lymphocyte-oriented kinase
• PRO2729
• serine/threonine kinase 10

Background

This gene encodes a member of the Ste20 family of serine/threonine protein kinases, and is similar to several known polo-like kinase kinases. The protein can associate with and phosphorylate polo-like kinase 1, and overexpression of a kinase-dead version of the protein interferes with normal cell cycle progression. The kinase can also negatively regulate interleukin 2 expression in T-cells via the mitogen activated protein kinase kinase 1 pathway. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: STK10
• Uniprot:
  • O94804(Human)