LMW-PTP/ACP1 Antibody (475417) [Unconjugated]

Western blot shows lysates of CHP-100 human neuroblastoma cell line and HepG2 human hepatocellular carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat LMW-PTP/ACP1 Monoclonal Antibody (Catalog # MAB5075) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for LMW-PTP/ACP1 at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: 475417
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

LMW-PTP/ACP1 Antibody (475417) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human LMW-PTP/ACP1
  Ala2-His158
  Accession # P24666
• Specificity: Detects human, mouse, and rat LMW-PTP/ACP1 in Western blots.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: ACP1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for LMW-PTP/ACP1 Antibody (475417) [Unconjugated]

• acid phosphatase 1, soluble
• acid phosphatase of erythrocyte
• ACP1
• Adipocyte acid phosphatase
• cytoplasmic phosphotyrosyl protein phosphatase
• EC 3.1.3.2
• EC 3.1.3.48
• HAAP
• LMWPTP
• LMW-PTP
• LMW-PTPase
• Low molecular weight cytosolic acid phosphatase
• low molecular weight phosphotyrosine protein phosphatase
• MGC111030
• MGC3499
• protein tyrosine phosphatase
• Red cell acid phosphatase 1

Background

Low molecular weight protein tyrosine phosphatase (LMW-PTP), also known as Acid Phosphatase 1 (ACP1), is an 18 kDa cytosolic phosphatase that is unrelated to other tyrosine phosphatases, such as PTP1B. Tyrosine phosphorylation of LMW-PTP increases its activity 20-fold and affects its ability to dephosphorylate targets such as the PDGF receptor. Cancer cells overexpressing LMW-PTP have a higher proliferative rate and generate tumors that are larger than untransfected controls. Colon adenocarcinomas induced by dimethylhydrazine also have elevated levels of LMW-PTP, suggesting that this phosphatase may be a marker for oncogenic transformation. Human LMW-PTP shares 86% and 87% amino acid sequence identity with mouse and rat LMW-PTP, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ACP1
• Uniprot:
  • P24666()