LMO2 Antibody (4D8) - BSA Free

Western Blot: LMO2 Antibody (4D8) [NBP2-37442] - Western blot analysis using LMO2 mAb against HEK293 (1) and LMO2 (AA: 1-158)-hIgGFc transfected HEK293 (2) cell lysate.

Flow Cytometry: LMO2 Antibody (4D8) [NBP2-37442] - An intracellular stain was performed on U-937 cells with LMO2 Antibody (4D8) NBP2-37442F (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to FITC.

Western Blot: LMO2 Antibody (4D8) [NBP2-37442] - Western blot analysis using LMO2 mAb against human LMO2 recombinant protein. (Expected MW is 43.9 kDa)

ELISA: LMO2 Antibody (4D8) [NBP2-37442] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

• Reactivity: Hu
• Applications: WB, ELISA, Flow, CyTOF-ready
• Clone: 4D8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

LMO2 Antibody (4D8) - BSA Free Summary

• Description: Novus Biologicals Mouse LMO2 Antibody (4D8) - BSA Free (NBP2-37442) is a monoclonal antibody validated for use in WB, ELISA and Flow. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Purified recombinant fragment of human LMO2 (AA: 1-158) expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: LMO2
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Western Blot 1:500 - 1:2000
• Application Notes: This antibody is Cytof ready.
• Theoretical MW: 18.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for LMO2 Antibody (4D8) - BSA Free

• Cysteine-rich protein TTG-2
• LIM domain only 2 (rhombotin-like 1)
• LIM domain only protein 2
• LMO2
• LMO-2
• RBTN2
• RBTN2rhombotin-2
• RBTNL1
• RBTNL1rhombotin-like 1
• RHOM2
• RHOM2T-cell translocation protein 2
• T-cell translocation gene 2
• TTG2
• TTG2rhombotin 2

Background

LMO2 is a highly conserved oncoprotein that is expressed in human normal germinal-center B cells and in B-cell lymphomas. LMO2 also has a crucial role in hematopoietic development by being necessary for yolk sac erythropoiesis. LMO2 encodes a cysteine-rich, two LIM-domain protein that is required for yolk sac erythropoiesis. The LMO2 transcription start site is located approximately 25 kb downstream from the 11p13 T-cell translocation cluster (11p13 ttc), where a number of T-cell acute lymphoblastic leukemia-specific translocations occur.

LMO2 antibodies are useful tools for erythropoiesis research and certian cancer studies.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: LMO2