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Livin Antibody (88C570) [DyLight 650]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clone
88C570
Clonality
Monoclonal
Host
Mouse
Conjugate
DyLight 650

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 405
NB100-56548AF405
Alexa Fluor 488
NB100-56548AF488
Alexa Fluor 647
NB100-56548AF647
Alexa Fluor 700
NB100-56548AF700
Biotin
NB100-56548B
DyLight 350
NB100-56548UV
DyLight 405
NB100-56548V
DyLight 488
NB100-56548G
DyLight 550
NB100-56548R
DyLight 680
NB100-56548FR
DyLight 755
NB100-56548IR
FITC
NB100-56548F
HRP
NB100-56548H
Unconjugated
NB100-56548
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NB100-56548
Azide Free
NBP2-33225
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Livin Antibody (88C570) [DyLight 650] Summary

Immunogen
Recombinant human livin protein was used as immunogen.
Specificity
The Livin antibody, clone 88C570 has been shown to recognize both Livin alpha (~39 kDa) and Livin beta (~37 kDa) isoforms (Nachmias et al, 2003). 2. The antibody also recognizes the 30 kDa (p30-Livin alpha) and 28 kDa (p28-Livin beta) apoptotic cleavage fragments of Livin alpha and beta, respectively (Nachmias et al, 2003). Apoptotic cleavage fragments of Livin have also been referred to as truncated livin (tLivin) (Nachmias et al, 2007). 3. The specificity of the antibody has been validated using Livin shRNA (Quiping et al, 2004).
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
BIRC7
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • Western Blot
  • Immunocytochemistry/Immunofluorescence
Application Notes
Immunocytochemistry/Immunofluorescence see Nachmias et al, 2007

Reactivity Notes

Cross reacts with Human.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Livin Antibody (88C570) [DyLight 650]

  • baculoviral IAP repeat containing 7
  • baculoviral IAP repeat-containing 7
  • BIRC7
  • KIAP
  • KIAPRING finger protein 50
  • Kidney inhibitor of apoptosis protein
  • livin inhibitor-of-apoptosis
  • Livin
  • Melanoma inhibitor of apoptosis protein
  • ML-IAP
  • MLIAPlivin inhibitor of apoptosis
  • ML-IAPmliap
  • RNF50LIVINbaculoviral IAP repeat-containing protein 7

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NAIP Antibody
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MAB829
Western blot shows lysates of HEK293 human embryonic kidney cell line either mock transfected or transfected with human NAIP and human brain (cerebellum) tissue. PVDF Membrane was probed with 1 µg/mL of Human NAIP Monoclonal Antibody (Catalog # MAB829) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for NAIP at approximately 160 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
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Bcl-2 Antibody
NB100-56098
Western Blot: Bcl-2 Antibody [NB100-56098] - Analysis of Bcl-2 in whole cell lysate from Daoy cells. Cells were transfected with scrambled control siRNA (lane 1) or Bcl-2 siRNA (lane2). Image from verified customer review.
Western Blot: Bcl-2 Antibody [NB100-56098] - WB analysis of whole cell lysate (WCL) of Jurkat cells using anti-Bcl-2 antibody (NB100-56098) at 1:1000 dilution. HRP conjugated goat anti-rabbit IgG (H+L) cross adsorbed secondary antibody was used with ECL substrate for the detection of Bcl2 antibody bound to the blotted protein. This Bcl2 antibody detected a specific band at expected molecular weight marker position of Bcl2 protein (26kDa).
Immunohistochemistry-Paraffin: Bcl-2 Antibody [NB100-56098] - IHC analysis of a formalin-fixed paraffin-embedded (FFPE) human breast carcinoma tissue section using 1:1000 dilution of Bcl-2 antibody (NB100-56098) on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) with 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching using peroxide block. The sections were incubated with primary antibody for 30 minutes. Bond Polymer Refine Detection (Leica Biosystems) and DAB were used for signal detection which followed counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (20X) were performed using Aperio AT2 (Leica Biosystems). This antibody generated a diffused cytoplasmic staining of BCL2 in the cancer cells with relatively intense signal in their nuclear membranes. Staining was performed by Histowiz.
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Bcl-xL Antibody
NB100-56104
Western Blot: Bcl-xL Antibody [NB100-56104] - Analysis of Bcl-xL in Daoy whole cell lysate using anti-Bcl-xL antibody. Image from verified customer review.
Immunohistochemistry-Paraffin: Bcl-Xl Antibody [NB100-56104] - Formalin-fixed paraffin-embedded sections of human epidermis stained for Bcl-X expression using this antibody at the bottom of the images were negative for Bcl-X expression. The adjacent stratum spinosum and middle stratum granulosum layers were both very positive for Bcl-X expression. The top stratum corneum consisting mainly of dead or peeling cells were both negative for Bcl-X expression.
Western Blot: Bcl-Xl Antibody [NB100-56104] - Analysis of Bcl-X in lysates from isolated rat liver mitochondria using this antibody. The antibody detected Bcl-XL at ~30 kDa.
(1 Review)
(1 Publication)
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Livin Antibody
(88C570) [DyLight 650
NB100-56548C
Livin Antibody (88C570) [DyLight 650]
Species: Hu
Applications: WB, ICC/IF
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Product General Protocols

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin Labeled NB100-56548B
DyLight 350 Labeled NB100-56548UV
DyLight 405 Labeled NB100-56548V
DyLight 488 Labeled NB100-56548G
DyLight 550 Labeled NB100-56548R
DyLight 680 Labeled NB100-56548FR
DyLight 755 Labeled NB100-56548IR
HRP Labeled NB100-56548H
Learn about Custom Antibody Labeling

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Bioinformatics Tool for Livin Antibody (NB100-56548C)

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Blogs on Livin.

Check out the latest blog posts on Livin.
Survivin - an inhibitor of apoptosis protein
Survivin is an anti-apoptotic protein which is the smallest protein within a large family of proteins including X-linked IAP, c-IAP1 and 2, IAP-like protein-2, melanoma IAP, NAIP, and Livin. Survivin is responsible for a wide range of basic cellula...  Read full blog post.
Survivin is thrivin'
The survivin anti-apoptotic protein is the smallest member of a large family of proteins such as X-linked IAP, c-IAP1 and 2, IAP-like protein-2, melanoma IAP, Livin, and NAIP. Survivin regulates basic physiological events such as the cell cycle, tumor...  Read full blog post.
Livin: On a Prayer
Livin is a member of the inhibitor of apoptosis proteins (IAP) family that regulates programmed cell death. The Livin protein contains a single baculovirus IAP repeat (BIR) essential for function, along with a COOH-terminal RING-type zinc finger domai...  Read full blog post.

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Bioinformatics

Gene Symbol BIRC7
Entrez