LITAF Antibody

Immunohistochemistry-Paraffin: LITAF Antibody [NBP1-00208] - (6.5ug/ml) staining of Thymus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu
• Applications: ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

LITAF Antibody Summary

• Immunogen: Peptide with sequence C-PDGKGMNPPSYYTQ corresponding to internal region according to NP_001129945.1.
• Specificity: This antibody is excpected to crossreact with both reported isoforms (NP_004853.2; NP_001129945.1).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: LITAF
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 6.5 ug/ml
  • Peptide ELISA Detection limit 1:8000
• Application Notes: WB: Preliminary experiments gave an approx. 25 kDa band in human lymph node lysates and in lysates of HeLa and HepG2 after 0.1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 17.1 kDa band according to NP_004853.2. The 25 kDa band was successfully blocked by incubation with the immunizing peptide.
• Control:

  LITAF Overexpression Lysate

Reactivity Notes

xpected from sequence similarity: Mouse, Rat

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for LITAF Antibody

• CMT1C
• FLJ38636
• lipopolysaccharide-induced TNF factor
• lipopolysaccharide-induced TNF-alpha factor
• lipopolysaccharide-induced tumor necrosis factor-alpha factor
• LITAF
• LPS-induced TNF-alpha factor
• MGC116698
• MGC116700
• MGC125275
• MGC125276
• p53-induced gene 7 protein
• PIG7
• PIG7MGC116701
• SIMPLE
• SIMPLEMGC125274
• Small integral membrane protein of lysosome/late endosome
• TP53I7
• tumor protein p53 inducible protein 7

Background

Lipopolysaccharide is a potent stimulator of monocytes and macrophages, causing secretion of tumor necrosis factor-alpha (TNF-alpha) and other inflammatory mediators. This gene encodes lipopolysaccharide-induced TNF-alpha factor, which is a DNA-binding protein and can mediate the TNF-alpha expression by direct binding to the promoter region of the TNF-alpha gene. The transcription of this gene is induced by tumor suppresor p53 and has been implicated in the p53-induced apoptotic pathway. Mutations in this gene cause Charcot-Marie-Tooth disease type 1C (CMT1C) and may be involved in the carcinogenesis of extramammary Paget's disease (EMPD). Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for LITAF Antibody (NBP1-00208)(1)

Publication using NBP1-00208

• Tang X, Metzger D, Leeman S, Amar S. LPS-induced TNF-alpha factor (LITAF)-deficient mice express reduced LPS-induced cytokine: Evidence for LITAF-dependent LPS signaling pathways. Proc Natl Acad Sci U S A 2006-09-12 [PMID: 16954198]

Bioinformatics

• Gene Symbol: LITAF
• Entrez:
  • 9516(Human)
  • 56722(Mouse)
  • 65161(Rat)
• Uniprot:
  • NP_004853.2(Human)